##gff-version 3
##sequence-region BBa_J202046 1 1830
BBa_J202046	.	promoter	1	54	.	+	0	ID=BBa_J202038;Name=BBa_J202038
BBa_J202046	.	sequence_alteration	29	29	.	+	0	ID=annotation2178856;Name=Mutation;Parent=BBa_J202038
BBa_J202046	.	sequence_alteration	16	16	.	+	0	ID=annotation2178855;Name=Mutation;Parent=BBa_J202038
BBa_J202046	.	operator	26	44	.	+	0	ID=annotation2178859;Name=O2;Parent=BBa_J202038
BBa_J202046	.	operator	1	19	.	+	0	ID=annotation2178858;Name=O2;Parent=BBa_J202038
BBa_J202046	.	sequence_alteration	4	4	.	+	0	ID=annotation2178854;Name=Mutation;Parent=BBa_J202038
BBa_J202046	.	sequence_alteration	41	41	.	+	0	ID=annotation2178857;Name=Mutation;Parent=BBa_J202038
BBa_J202046	.	ribosome_entry_site	990	1002	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_J202046	.	ribosome_entry_site	996	999	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J202046	.	ribosome_entry_site	990	1002	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J202046	.	engineered_region	990	1002	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J202046	.	engineered_region	63	938	.	+	0	ID=BBa_E0240;Name=BBa_E0240
BBa_J202046	.	CDS	82	801	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0240
BBa_J202046	.	CDS	82	801	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J202046	.	terminator	898	938	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0240
BBa_J202046	.	engineered_region	898	938	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J202046	.	stop_codon	931	931	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J202046	.	polyA_site	925	938	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J202046	.	stem_loop	905	924	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J202046	.	terminator	810	889	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0240
BBa_J202046	.	engineered_region	810	889	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J202046	.	stem_loop	821	864	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J202046	.	ribosome_entry_site	63	75	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_E0240
BBa_J202046	.	ribosome_entry_site	69	72	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J202046	.	ribosome_entry_site	63	75	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J202046	.	engineered_region	63	75	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J202046	.	promoter	947	981	.	+	0	ID=BBa_J23101;Name=BBa_J23101
BBa_J202046	.	CDS	1009	1693	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_J202046	.	tag	1629	1662	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J202046	.	engineered_tag	1669	1693	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J202046	.	CDS	1012	1628	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J202046	.	terminator	1702	1830	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J202046	.	terminator	1790	1830	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J202046	.	engineered_region	1790	1830	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J202046	.	stop_codon	1823	1823	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J202046	.	polyA_site	1817	1830	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J202046	.	stem_loop	1797	1816	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J202046	.	terminator	1702	1781	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J202046	.	engineered_region	1702	1781	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J202046	.	stem_loop	1713	1756	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_J202046
tccgtatcagtgatacagattgacatccgtatcagtgatacagatactgagcactactagagtcacacaggaaagtact
agatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaa
attttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaa
ctacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatga
aacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaa
ctacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaa
gaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaa
agaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaaca
aaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagat
cccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactat
acaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtt
tacagctagctcagtcctaggtattatgctagctactagagtcacacaggaaagtactagatgtccagattagataaaa
gtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaa
gctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatg
ttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagtt
ttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaac
tctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtgggg
cattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgata
gtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttga
attgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagta
gcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaaga
ctgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcct
ttctgcgtttata