##gff-version 3
##sequence-region BBa_J202019 1 1014
BBa_J202019	.	engineered_region	139	1014	.	+	0	ID=BBa_E0240;Name=BBa_E0240
BBa_J202019	.	CDS	158	877	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0240
BBa_J202019	.	CDS	158	877	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J202019	.	terminator	974	1014	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0240
BBa_J202019	.	engineered_region	974	1014	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J202019	.	stop_codon	1007	1007	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J202019	.	polyA_site	1001	1014	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J202019	.	stem_loop	981	1000	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J202019	.	terminator	886	965	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0240
BBa_J202019	.	engineered_region	886	965	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J202019	.	stem_loop	897	940	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J202019	.	ribosome_entry_site	139	151	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_E0240
BBa_J202019	.	ribosome_entry_site	145	148	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J202019	.	ribosome_entry_site	139	151	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J202019	.	engineered_region	139	151	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J202019	.	promoter	1	130	.	+	0	ID=BBa_J202003;Name=BBa_J202003
BBa_J202019	.	sequence_alteration	120	120	.	+	0	ID=annotation2178431;Name=Mutation 3;Parent=BBa_J202003
BBa_J202019	.	non_covalent_binding_site	61	77	.	+	0	ID=annotation2178428;Name=araI2;Parent=BBa_J202003
BBa_J202019	.	sequence_alteration	70	70	.	+	0	ID=annotation2178430;Name=Mutation 2;Parent=BBa_J202003
BBa_J202019	.	non_covalent_binding_site	40	56	.	+	0	ID=annotation2178427;Name=araI1;Parent=BBa_J202003
BBa_J202019	.	start_codon	112	112	.	+	0	ID=annotation2178426;Name=Transcription Start;Parent=BBa_J202003
BBa_J202019	.	sequence_alteration	49	49	.	+	0	ID=annotation2178429;Name=Mutation 1;Parent=BBa_J202003
>BBa_J202019
acattgattatttgcacggcgtcacactttgctatgccatagcattttcatccataagattagcggatcatacctgacg
ctttttatcgcaactctctactgtttctccataccgttttattgggctagctactagagtcacacaggaaagtactaga
tgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaatt
ttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaacta
cctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaac
agcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaacta
caagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaa
gatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaaga
atggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaa
tactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatccc
aacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactataca
aataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt
tgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata