##gff-version 3
##sequence-region BBa_J176149 1 5696
BBa_J176149	.	engineered_region	1	2253	.	+	0	ID=BBa_J176098;Name=BBa_J176098
BBa_J176149	.	engineered_region	573	2253	.	+	0	ID=BBa_S04739;Name=BBa_S04739;Parent=BBa_J176098
BBa_J176149	.	engineered_region	573	2022	.	+	0	ID=BBa_S04738;Name=BBa_S04738;Parent=BBa_S04739
BBa_J176149	.	engineered_region	597	2022	.	+	0	ID=BBa_S04684;Name=BBa_S04684;Parent=BBa_S04738
BBa_J176149	.	sequence_feature	1977	1982	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04684
BBa_J176149	.	sequence_feature	2004	2009	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04684
BBa_J176149	.	tag	1983	2003	.	+	0	ID=BBa_J176008;Name=BBa_J176008;Parent=BBa_S04684
BBa_J176149	.	polypeptide_domain	597	1283	.	+	0	ID=BBa_J176022;Name=BBa_J176022;Parent=BBa_S04684
BBa_J176149	.	sequence_feature	1284	1289	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04684
BBa_J176149	.	polypeptide_domain	1290	1976	.	+	0	ID=BBa_J176022;Name=BBa_J176022;Parent=BBa_S04684
BBa_J176149	.	terminator	2010	2022	.	+	0	ID=BBa_J176016;Name=BBa_J176016;Parent=BBa_S04684
BBa_J176149	.	sequence_feature	591	596	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04738
BBa_J176149	.	ribosome_entry_site	573	590	.	+	0	ID=BBa_J176012;Name=BBa_J176012;Parent=BBa_S04738
BBa_J176149	.	sequence_feature	2023	2028	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04739
BBa_J176149	.	tag	2029	2253	.	+	0	ID=BBa_J176009;Name=BBa_J176009;Parent=BBa_S04739
BBa_J176149	.	sequence_feature	567	572	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_J176098
BBa_J176149	.	engineered_region	1	566	.	+	0	ID=BBa_S04740;Name=BBa_S04740;Parent=BBa_J176098
BBa_J176149	.	sequence_feature	468	473	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04740
BBa_J176149	.	sequence_feature	474	566	.	+	0	ID=BBa_J176019;Name=BBa_J176019;Parent=BBa_S04740
BBa_J176149	.	non_covalent_binding_site	512	528	.	+	0	ID=annotation2159561;Name=Gal4;Parent=BBa_J176019
BBa_J176149	.	non_covalent_binding_site	550	566	.	+	0	ID=annotation2159563;Name=Gal4;Parent=BBa_J176019
BBa_J176149	.	non_covalent_binding_site	493	509	.	+	0	ID=annotation2159560;Name=Gal4;Parent=BBa_J176019
BBa_J176149	.	non_covalent_binding_site	531	547	.	+	0	ID=annotation2159562;Name=Gal4;Parent=BBa_J176019
BBa_J176149	.	non_covalent_binding_site	474	490	.	+	0	ID=annotation2159559;Name=Gal4;Parent=BBa_J176019
BBa_J176149	.	engineered_region	1	467	.	+	0	ID=BBa_S04642;Name=BBa_S04642;Parent=BBa_S04740
BBa_J176149	.	sequence_feature	369	374	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04642
BBa_J176149	.	promoter	1	368	.	+	0	ID=BBa_J176003;Name=BBa_J176003;Parent=BBa_S04642
BBa_J176149	.	sequence_feature	375	467	.	+	0	ID=BBa_J176019;Name=BBa_J176019;Parent=BBa_S04642
BBa_J176149	.	non_covalent_binding_site	413	429	.	+	0	ID=annotation2159561;Name=Gal4;Parent=BBa_J176019
BBa_J176149	.	non_covalent_binding_site	451	467	.	+	0	ID=annotation2159563;Name=Gal4;Parent=BBa_J176019
BBa_J176149	.	non_covalent_binding_site	394	410	.	+	0	ID=annotation2159560;Name=Gal4;Parent=BBa_J176019
BBa_J176149	.	non_covalent_binding_site	432	448	.	+	0	ID=annotation2159562;Name=Gal4;Parent=BBa_J176019
BBa_J176149	.	non_covalent_binding_site	375	391	.	+	0	ID=annotation2159559;Name=Gal4;Parent=BBa_J176019
BBa_J176149	.	sequence_feature	2254	2259	.	+	0	ID=BBa_J176041;Name=BBa_J176041
BBa_J176149	.	engineered_region	2260	5696	.	+	0	ID=BBa_S04736;Name=BBa_S04736
BBa_J176149	.	engineered_region	5279	5696	.	+	0	ID=BBa_S04730;Name=BBa_S04730;Parent=BBa_S04736
BBa_J176149	.	tag	5472	5696	.	+	0	ID=BBa_J176009;Name=BBa_J176009;Parent=BBa_S04730
BBa_J176149	.	mature_transcript_region	5279	5465	.	+	0	ID=BBa_J176025;Name=BBa_J176025;Parent=BBa_S04730
BBa_J176149	.	sequence_feature	5333	5351	.	+	0	ID=annotation2166808;Name=repeat 2;Parent=BBa_J176025
BBa_J176149	.	sequence_feature	5413	5431	.	+	0	ID=annotation2166811;Name=repeat 5;Parent=BBa_J176025
BBa_J176149	.	sequence_feature	5306	5324	.	+	0	ID=annotation2166807;Name=repeat 1;Parent=BBa_J176025
BBa_J176149	.	sequence_feature	5360	5378	.	+	0	ID=annotation2166809;Name=repeat 3;Parent=BBa_J176025
BBa_J176149	.	sequence_feature	5387	5405	.	+	0	ID=annotation2166810;Name=repeat 4;Parent=BBa_J176025
BBa_J176149	.	sequence_feature	5466	5471	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04730
BBa_J176149	.	sequence_feature	5273	5278	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04736
BBa_J176149	.	engineered_region	2260	5272	.	+	0	ID=BBa_S04732;Name=BBa_S04732;Parent=BBa_S04736
BBa_J176149	.	sequence_feature	3265	3270	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04732
BBa_J176149	.	sequence_feature	5254	5259	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04732
BBa_J176149	.	sequence_feature	2800	2805	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04732
BBa_J176149	.	tag	3271	3804	.	+	0	ID=BBa_J176018;Name=BBa_J176018;Parent=BBa_S04732
BBa_J176149	.	polypeptide_domain	2806	3264	.	+	0	ID=BBa_J176020;Name=BBa_J176020;Parent=BBa_S04732
BBa_J176149	.	sequence_feature	5227	5232	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04732
BBa_J176149	.	tag	5233	5253	.	+	0	ID=BBa_J176008;Name=BBa_J176008;Parent=BBa_S04732
BBa_J176149	.	terminator	5260	5272	.	+	0	ID=BBa_J176016;Name=BBa_J176016;Parent=BBa_S04732
BBa_J176149	.	polypeptide_domain	3811	4515	.	+	0	ID=BBa_J176005;Name=BBa_J176005;Parent=BBa_S04732
BBa_J176149	.	sequence_feature	4516	4521	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04732
BBa_J176149	.	sequence_feature	2776	2781	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04732
BBa_J176149	.	ribosome_entry_site	2782	2799	.	+	0	ID=BBa_J176012;Name=BBa_J176012;Parent=BBa_S04732
BBa_J176149	.	promoter	2260	2775	.	+	0	ID=BBa_J176028;Name=BBa_J176028;Parent=BBa_S04732
BBa_J176149	.	sequence_alteration	2770	2770	.	+	0	ID=annotation2166554;Name=mutated PstI;Parent=BBa_J176028
BBa_J176149	.	sequence_alteration	2512	2512	.	+	0	ID=annotation2166553;Name=mutated SpeI;Parent=BBa_J176028
BBa_J176149	.	polypeptide_domain	4522	5226	.	+	0	ID=BBa_J176005;Name=BBa_J176005;Parent=BBa_S04732
BBa_J176149	.	sequence_feature	3805	3810	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04732
>BBa_J176149
aaactggcctccgcgccgggcttttggcgcctcccgcgggcgcccccctcctcacggcgagcgctgccacgtcagacga
agggcgcagcgagcgtcctgatccttccgcccggacgctcaggacagcggcccgctgctcataagactcggccttagaa
ccccagtatcagcagaaggacattttaggacgggacttgggtgactctagggcactggttttctttccagagagcggaa
caggcgaggaaaagtagtcccttctcggcgattctgcggagggatctccgtggggcggtgaacgccgatgattatataa
ggacgcgccgggtgtggcacagctagttactagacccgccgccaccatggagactagacggagtactgtcctccgagcg
gagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgactagac
ggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcgga
gtactgtcctccgactagacccgccgccaccatggagactagaatggccctgtccaacaagttcatcggcgacgacatg
aagatgacctaccacatggacggctgcgtgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacg
agggcacccagacctccaccttcaaggtgaccatggccaacggcggccccctggccttctccttcgacatcctgtccac
cgtgttcatgtacggcaaccgctgcttcaccgcctaccccaccagcatgcccgactacttcaagcaggccttccccgac
ggcatgtcctacgagagaaccttcacctacgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggca
actgcttcgagcacaagtccaccttccacggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccgg
ctgggacccctccttcgagaagatgaccgtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcaa
ggcggcggcaactacagatgccagttccacacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtgg
tggagcaccgcatcgccagaaccgacctggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacat
cacctccgtggtgcccttcactagaatggccctgtccaacaagttcatcggcgacgacatgaagatgacctaccacatg
gacggctgcgtgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacgagggcacccagacctcca
ccttcaaggtgaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatgtacggcaa
ccgctgcttcaccgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacggcatgtcctacgagaga
accttcacctacgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaactgcttcgagcacaagt
ccaccttccacggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggctgggacccctccttcga
gaagatgaccgtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcaaggcggcggcaactacaga
tgccagttccacacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccgcatcgcca
gaaccgacctggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtggtgccctt
cactagacccaagaaaaagcgcaaggtaactagagcgtaaagctgagactagactgtgccttctagttgccagccatct
gttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaa
ttgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattggga
agacaatagcaggcatgctggggatgcggtgggctctatggactagactaccgggtaggggaggcgcttttcccaaggc
agtctggagcatgcgctttagcagccccgctgggcacttggcgctacacaagtggcctctggcctcgcacacattccac
atccaccggtaggcgccaaccggctccgttctttggtggccccttcgcgccaccttctactcctcccctagtcaggaag
ttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatggaagtagcacgtctcacaagtctcgtgcagatgg
acagcaccgctgagcaatggaagcgggtaggcctttggggcagcggccaatagcagctttgctccttcgctttctgggc
tcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggctcaggggcggggcgggcgcccgaaggtcctcc
ggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgctgttctcctcttcctcatctccgggcctttcga
cctggagcccactagacccgccgccaccatggagactagaatgaagctactgtcttctatcgaacaagcatgcgatatt
tgccgacttaaaaagctcaagtgctccaaagaaaaaccgaagtgcgccaagtgtctgaagaacaactgggagtgtcgct
actctcccaaaaccaaaaggtctccgctgactagggcacatctgacagaagtggaatcaaggctagaaagactggaaca
gctatttctactgatttttcctcgagaagaccttgacatgattttgaaaatggattctttacaggatataaaagcattg
ttaacaggattatttgtacaagataatgtgaataaagatgccgtcacagatagattggcttcagtggagactgatatgc
ctctaacattgagacagcatagaataagtgcgacatcatcatcggaagagagtagtaacaaaggtcaaagacagttgac
tgtatcgccggaatttccggggatcactagagcatccaacttgaacatttcgagaaagcttaccatatcaaccccatca
tgctctttcgaaaattcaaatagcacatccattccttcgcccgcttcctcatctcaaagccacactccaatgagaaaca
tgagctcactctctgataacagcgttttcagccggaatatggaacaatcatcaccaatcactccaagtatgtaccaatt
tggtcagcagcagtcaaacagtatatgtggtagcaccgttagtgtgaatagtctggtgaatacaaataacaaacaaagg
atctacgaacaaatcacgggtcctaacagcaataacgcaaccaatgattatattgatttgctaaacctaaatgagtcta
acaaggaaaaccaaaatcccgcaacggcgcattacctcaatgggggcccacccaagacaagcttcattaaccatggaat
gttcccctcgccaactgggaccataaatagcggtaaatctagcagtgcctcatctttaatttcttttggtatgggcaat
acccaagtaataactagagtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgc
acatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagac
cgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctcc
aaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcg
tgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggt
gaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgag
cggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacg
ctgaggtcaagaccacctacaaggccaagaagcccgtgcagctacccggcgcctacaacgtcaacatcaagttggacat
cacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggac
gagctgtacaagactagagtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgc
acatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagac
cgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctcc
aaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcg
tgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggt
gaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgag
cggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacg
ctgaggtcaagaccacctacaaggccaagaagcccgtgcagctacccggcgcctacaacgtcaacatcaagttggacat
cacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggac
gagctgtacaagactagacccaagaaaaagcgcaaggtaactagagcgtaaagctgagactagaatagttgtttaaacg
agctcgctagcaaatcaagagtcctcataaaggaattaaaatcaagagtcctcataaagtctagcaaatcaagagtcct
cataaagctagacaaatcaagagtcctcataaataattccaatcaagagtcctcataaagtcgacctgcaagcatgcaa
gctgatccggctgcactagactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgacc
ctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattcta
ttctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtggg
ctctatgg