##gff-version 3
##sequence-region BBa_J13075 1 1680
BBa_J13075	.	terminator	1552	1631	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J13075	.	engineered_region	1552	1631	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J13075	.	stem_loop	1563	1606	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J13075	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J13075	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J13075	.	CDS	821	1543	.	+	0	ID=BBa_E0030;Name=BBa_E0030
BBa_J13075	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J13075	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J13075	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J13075	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J13075	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J13075	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J13075	.	engineered_region	81	794	.	+	0	ID=BBa_J06504;Name=BBa_J06504
BBa_J13075	.	CDS	81	791	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_J13075	.	sequence_alteration	432	432	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_J13075	.	ribosome_entry_site	803	814	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J13075	.	conserved_region	807	810	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J13075	.	terminator	1640	1680	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J13075	.	engineered_region	1640	1680	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J13075	.	stem_loop	1647	1666	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J13075	.	polyA_site	1667	1680	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J13075	.	stop_codon	1673	1673	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
>BBa_J13075
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctcc
gtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaagg
tgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaa
gcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgag
gacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggca
ccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccga
ggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagacc
acctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacg
aggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagta
ataatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctgg
tcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagct
gaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctg
caatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccagg
agcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaa
ccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagc
cacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacg
gcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccacta
cctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgcc
gccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcag
tcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
gtgggcctttctgcgtttata