##gff-version 3
##sequence-region BBa_J13050 1 1690
BBa_J13050	.	CDS	831	1553	.	+	0	ID=BBa_E0030;Name=BBa_E0030
BBa_J13050	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J13050	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J13050	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J13050	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J13050	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J13050	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J13050	.	terminator	1562	1641	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J13050	.	stem_loop	1573	1616	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J13050	.	engineered_region	1562	1641	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J13050	.	terminator	1650	1690	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J13050	.	engineered_region	1650	1690	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J13050	.	stop_codon	1683	1683	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J13050	.	polyA_site	1677	1690	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J13050	.	stem_loop	1657	1676	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J13050	.	ribosome_entry_site	812	824	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_J13050	.	ribosome_entry_site	812	824	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J13050	.	engineered_region	812	824	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J13050	.	ribosome_entry_site	818	821	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J13050	.	CDS	81	803	.	+	0	ID=BBa_E0020;Name=BBa_E0020
BBa_J13050	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J13050	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_J13050
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccac
aagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggca
agctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacat
gaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggc
aactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttca
aggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagca
gaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccag
cagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaag
accccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagct
gtacaagtaataatactagagtcacacaggaaagtactagatggtgagcaagggcgaggagctgttcaccggggtggtg
cccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacct
acggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcgg
ctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggc
tacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgaca
ccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaa
ctacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaac
atcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccg
acaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagtt
cgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttata