##gff-version 3
##sequence-region BBa_J13000 1 571
BBa_J13000	.	engineered_region	64	571	.	+	0	ID=BBa_E0433;Name=BBa_E0433
BBa_J13000	.	terminator	443	571	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_E0433
BBa_J13000	.	terminator	443	522	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J13000	.	engineered_region	443	522	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J13000	.	stem_loop	454	497	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J13000	.	terminator	531	571	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J13000	.	engineered_region	531	571	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J13000	.	polyA_site	558	571	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J13000	.	stem_loop	538	557	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J13000	.	stop_codon	564	564	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J13000	.	engineered_region	82	434	.	+	0	ID=BBa_E0033;Name=BBa_E0033;Parent=BBa_E0433
BBa_J13000	.	CDS	82	429	.	+	0	ID=annotation308320;Name=lacZ alpha;Parent=BBa_E0033
BBa_J13000	.	promoter	254	272	.	+	0	ID=annotation1938940;Name=T7 promoter;Parent=BBa_E0033
BBa_J13000	.	sequence_alteration	168	168	.	+	0	ID=annotation308381;Name=A;Parent=BBa_E0033
BBa_J13000	.	sequence_feature	82	96	.	+	0	ID=annotation1938942;Name=lacZ gene fragment;Parent=BBa_E0033
BBa_J13000	.	promoter	107	116	.	+	0	ID=annotation1938939;Name=T3 promoter;Parent=BBa_E0033
BBa_J13000	.	sequence_alteration	162	162	.	+	0	ID=annotation308375;Name=T;Parent=BBa_E0033
BBa_J13000	.	sequence_feature	280	429	.	+	0	ID=annotation1938941;Name=lacZ gene fragment;Parent=BBa_E0033
BBa_J13000	.	sequence_alteration	189	189	.	+	0	ID=annotation308383;Name=C;Parent=BBa_E0033
BBa_J13000	.	sequence_alteration	192	192	.	+	0	ID=annotation308388;Name=G;Parent=BBa_E0033
BBa_J13000	.	sequence_alteration	159	159	.	+	0	ID=annotation308387;Name=C;Parent=BBa_E0033
BBa_J13000	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0433
BBa_J13000	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J13000	.	promoter	1	55	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_J13000	.	promoter	20	25	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J13000	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J13000	.	promoter	42	47	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J13000	.	engineered_region	1	55	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J13000	.	start_codon	53	53	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
>BBa_J13000
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatact
agatgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctccaccgcggtggcggc
agcactagagctagtggatcccccgggctgtagaaattcgatatcaagcttatcgataccgtcgacctcgagggggggc
ccggtacccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaa
accctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcac
cgatcgcccttcccaacagttgcgcagcctgaataataatactagagccaggcatcaaataaaacgaaaggctcagtcg
aaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtg
ggcctttctgcgtttata