##gff-version 3
##sequence-region BBa_J119346 1 5716
BBa_J119346	.	engineered_region	4648	5716	.	+	0	ID=BBa_J04450;Name=BBa_J04450
BBa_J119346	.	ribosome_entry_site	4856	4867	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_J119346	.	conserved_region	4860	4863	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J119346	.	CDS	4874	5554	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_J119346	.	CDS	4874	5548	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_J119346	.	engineered_tag	5555	5579	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_J119346	.	promoter	4648	4847	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_J119346	.	engineered_region	4648	4847	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J119346	.	start_codon	4820	4820	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J119346	.	non_covalent_binding_site	4736	4773	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J119346	.	promoter	4808	4813	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J119346	.	CDS	4648	4735	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J119346	.	non_covalent_binding_site	4813	4847	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J119346	.	promoter	4784	4789	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J119346	.	terminator	5676	5716	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_J119346	.	engineered_region	5676	5716	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J119346	.	stop_codon	5709	5709	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J119346	.	polyA_site	5703	5716	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J119346	.	stem_loop	5683	5702	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J119346	.	terminator	5588	5667	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_J119346	.	engineered_region	5588	5667	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J119346	.	stem_loop	5599	5642	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J119346	.	engineered_region	1	4639	.	+	0	ID=BBa_J119318;Name=BBa_J119318
BBa_J119346	.	engineered_region	3283	4639	.	+	0	ID=BBa_J119140;Name=BBa_J119140;Parent=BBa_J119318
BBa_J119346	.	promoter	3283	3335	.	+	0	ID=annotation2214183;Name=T5 promoter;Parent=BBa_J119140
BBa_J119346	.	operator	3336	3356	.	+	0	ID=annotation2214184;Name=lac operator;Parent=BBa_J119140
BBa_J119346	.	stop_codon	4633	4635	.	+	0	ID=annotation2214188;Name=Stop codon;Parent=BBa_J119140
BBa_J119346	.	start_codon	3442	3444	.	+	0	ID=annotation2214186;Name=Start codon;Parent=BBa_J119140
BBa_J119346	.	CDS	3442	4632	.	+	0	ID=annotation2214187;Name=TetA tetracycline resistance gene;Parent=BBa_J119140
BBa_J119346	.	stem_loop	3405	3464	.	+	0	ID=annotation2214185;Name=riboswitch;Parent=BBa_J119140
BBa_J119346	.	engineered_region	1	3274	.	+	0	ID=BBa_J119303;Name=BBa_J119303;Parent=BBa_J119318
BBa_J119346	.	promoter	1	36	.	+	0	ID=annotation2329360;Name=J119031;Parent=BBa_J119303
BBa_J119346	.	sequence_feature	122	3274	.	+	0	ID=annotation2329362;Name=eCDM8;Parent=BBa_J119303
BBa_J119346	.	ribosome_entry_site	37	124	.	+	0	ID=annotation2329361;Name=J119028;Parent=BBa_J119303
>BBa_J119346
ttgacaattaatcatccggctcgtaatttatgtggagggcccaagttcacttaaaaaggagatcaacaatgaaagcaat
tttcgtactgaaacatcttaatcatgcgatggacggtttctaatgaccatcaaagaaatgccgcagccgaaaacctttg
gtgaactgaaaaatctgccgctgctgaataccgataaaccggttcatgcactgatgaaaattgcagatgaactgggcga
gatctttaaattcgaagcaccgggtagcgttacccgttatctgagcagccagcgtctgattaaagaagcatgcgacgaa
agccgctttgataaaaatctgattcagtggctgaaattcatccgcgattttctgggtgatggtctgagcaccagctgga
cccatgaaaaaaactggaaaaaagcccataatattctgctgccgagctttagccagcaggcaatgaaaggttatcatgc
aatgatggttgatattgccgttcagctggttcagaaatgggaacgtctgaatgcagatgaacatattgaagttccggaa
gatatgacccgtctgaccctggataccattggtctgtgtggttttaactatcgcttcaacagcttttatcgtgatcagc
cgcatccgtttgttaccagcatggttcgtgcactggatgaagcagtgaataaatggcagcgtgcaaatccggatgatcc
ggcatatgatgaaaataaacgtcagttccaagaggatattaaagtgatgaatgacctggtggacaaaattatcgcagat
cgtaaagcaagcggtgaacagagtgatgatctgctgacccatatgctgaatggtaaagatccggaaaccggtgaaccgc
tggatgatgaaaacattcgttatcagattattaccttcctgattgccggtcatgaaaccaccagtggtctgctgagctt
tgcactgtattttctggttaaaaatccgcacgttctgcaaaaagcagccgaagaagcagcacgcgttctggttgatccg
gttccgagctataaacaggttaaacagctgaaatatgtgggcatggttctgaatgaagcactgcgtctgtggccgacct
ttccgtggtttagcctgtatgcaaaagaagataccgttctgggaggtgaatatccgctggaaaaaggtgatgaactgat
ggttctgattccgcagctgcatcgtgataaaaccatttggggtgatgatgtggaagaatttcgtccggaacgttttgaa
aatccgagcgcaattccgcagcatgcctttaaaccgtttggtaatggtcagcgtgcatgtattggtcagcagtttgcac
tgcatgaagccaccctggttctgggtatgatgctgaaacattttgattttgaggaccacaccaactatgagctggatat
taaaggcaccctgaccctgaaaccggaaggttttgttgttaaagccaaaagcaaaaaaatcccgctgggtggtattccg
agcccgagcaccgaacagagcgcaaaaaaagttcgtaaaaaagccgaaaacgcccataatacaccgctgctggttctgt
atggtagcaatatgggtacagccgaaggcaccgcacgtgatctggcagatattgcaatgagcaaaggttttgcaccgca
ggttgcaaccctggatagccatgcaggtaatctgcctcgtgaaggtgcagttctgattgttaccgcaagctataatggt
catccgcctgataatgcaaaacagtttgttgattggctggatcaggcaagcgcagatgaagttaaaggtgttcgttata
gcgtttttggttgcggtgataaaaactgggcaaccacctatcagaaagttccggcatttattgatgaaaccctggcagc
aaaaggtgcagaaaatattgcagatcgtggtgaaaccgatgccagtgatgattttgaaggcacctatgaagaatggcgt
gaacatatgtggtcagatgttgcagcctattttaacctggatatcgaaaacagcgaggacaataaaagcaccctgagcc
tgcaatttgtggatagcgcagcagatatgccgctggcaaaaatgcatggtgcatttagcaccaatgttgttgcaagcaa
agaactgcaacagcctggtagcgcacgtagcacccgtcatctggaaattgaactgccgaaagaagcaagctatcaagag
ggtgatcatctgggtgttattccgcgtaattatgaaggtattgttaatcgtgttaccgcacgttttggtctggatgcaa
gccagcagattcgtctggaagcagaagaagaaaaactggcacatctgccgctggccaaaaccgttagcgttgaagaact
gctgcaatatgttgaactgcaagatccggttacccgtacccagctgcgtgcaatggcagcaaaaaccgtttgtccgcct
cataaagttgaactggaagcactgctggagaaacaggcatataaagaacaggttctggcaaaacgtctgaccatgctgg
aactgctggaaaaatatccggcatgcgaaatgaaattcagcgaatttattgcactgctgccgagtattcgtccgcgtta
ttatagcattagcagcagtccgcgtgttgatgaaaaacaggccagcattaccgttagcgtggttagcggtgaagcatgg
tcaggttatggtgaatataaaggtattgccagcaattatctggccgaactgcaagaaggtgataccattacctgtttta
ttagcacaccgcagagcgaatttaccctgccgaaagatcctgaaacaccgctgattatggttggtccgggtacaggtgt
tgcaccgtttcgtggttttgttcaggcacgtaaacaactgaaagaacagggtcagagcctgggtgaagcacatctgtat
tttggttgtcgtagtccgcatgaggattatctgtatcaagaagaactggaaaacgcacagagcgaaggtattattaccc
tgcataccgcatttagccgtatgccgaatcagccgaaaacatatgttcagcatgttatggaacaggatggcaaaaaact
gattgaactgctggatcagggtgcccatttctatatttgtggtgatggtagccagatggcaccggcagttgaagcaacc
ctgatgaaaagctatgcagatgttcatcaggttagcgaagcagatgcacgtctgtggctgcaacagctggaagaaaaag
gtcgttatgcaaaagatgtttgggcaggttaataatactagagaaatcataaaaaatttatttgctttgtgagcggata
acaattataatagattcaattgtgagcggataacaattactagagatacgactcactataggtaccggtgataccagca
tcgtcttgatgcccttggcagcaccctgctaaggtaacaacaagatgcttaaatctaacaatgcgctcatcgtcatcct
cggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccat
tccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcg
gagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcat
ggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgct
ggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgg
gtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgct
caacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgaga
gccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatca
tgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgat
cggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttc
ggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggct
ggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggca
ggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctg
atcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttg
tctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaagcgttactagagcaatacgcaaaccg
cctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgca
acgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtgg
aattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatca
aagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaagg
tcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctg
tccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcc
cggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgca
agacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaacc
atgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaac
tgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgctta
caaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggt
cgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaa
ggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctca
ccttcgggtgggcctttctgcgtttata