##gff-version 3
##sequence-region BBa_J119136 1 1632
BBa_J119136	.	engineered_region	1	741	.	+	0	ID=BBa_J119135;Name=BBa_J119135
BBa_J119136	.	ribosome_entry_site	727	741	.	+	0	ID=annotation2213818;Name=RBS reverse;Parent=BBa_J119135
BBa_J119136	.	CDS	1	719	.	+	0	ID=annotation2213819;Name=GFP reverse;Parent=BBa_J119135
BBa_J119136	.	engineered_region	750	1632	.	+	0	ID=BBa_J119127;Name=BBa_J119127
BBa_J119136	.	stop_codon	950	952	.	+	0	ID=annotation2214066;Name=Stop for BD18 leader;Parent=BBa_J119127
BBa_J119136	.	sequence_feature	750	753	.	+	0	ID=annotation2214058;Name=BsmBI sticky end left;Parent=BBa_J119127
BBa_J119136	.	sequence_feature	755	760	.	+	0	ID=annotation2214059;Name=BsmBI cuts left;Parent=BBa_J119127
BBa_J119136	.	CDS	952	1632	.	+	0	ID=annotation2214067;Name=RFP E1010;Parent=BBa_J119127
BBa_J119136	.	sequence_feature	867	954	.	+	0	ID=annotation2214063;Name=BD18 biscistron;Parent=BBa_J119127
BBa_J119136	.	start_codon	952	954	.	+	0	ID=annotation2214068;Name=Start for RFP;Parent=BBa_J119127
BBa_J119136	.	stop_codon	1627	1632	.	+	0	ID=annotation2214069;Name=Double stop for E1010;Parent=BBa_J119127
BBa_J119136	.	ribosome_entry_site	884	892	.	+	0	ID=annotation2214064;Name=leader RBS;Parent=BBa_J119127
BBa_J119136	.	ribosome_entry_site	937	945	.	+	0	ID=annotation2214065;Name=RBS for RFP;Parent=BBa_J119127
BBa_J119136	.	stem_loop	761	855	.	+	0	ID=annotation2214060;Name=TT B0014;Parent=BBa_J119127
BBa_J119136	.	sequence_feature	856	861	.	+	0	ID=annotation2214061;Name=BsmBI cuts right;Parent=BBa_J119127
BBa_J119136	.	sequence_feature	863	866	.	+	0	ID=annotation2214062;Name=BsmBI sticky end right;Parent=BBa_J119127
>BBa_J119136
ttattatttgtatagttcatccatgccatgtgtaatcccagcagctgttacaaactcaagaaggaccatgtggtctctc
ttttcgttgggatctttcgaaagggcagattgtgtggacaggtaatggttgtctggtaaaaggacagggccatcgccaa
ttggagtattttgttgataatggtctgctagttgaacgcttccatcttcaatgttgtgtctaattttgaagttaacttt
gattccattcttttgtttgtctgccatgatgtatacattgtgtgagttatagttgtattccaatttgtgtccaagaatg
tttccatcttctttaaaatcaataccttttaactcgattctattaacaagggtatcaccttcaaacttgacttcagcac
gtgtcttgtagttcccgtcatctttgaaaaatatagttctttcctgtacataaccttcgggcatggcactcttgaaaaa
gtcatgctgtttcatatgatctgggtatctcgcaaagcattgaacaccataaccgaaagtagtgacaagtgttggccat
ggaacaggtagttttccagtagtgcaaataaatttaagggtaagttttccgtatgttgcatcaccttcaccctctccac
tgacagaaaatttgtgcccattaacatcaccatctaattcaacaagaattgggacaactccagtgaaaagttcttctcc
tttacgcatctagtatttctcctctttaattactagagcgactgagacgtcacactggctcaccttcgggtgggccttt
ctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattatttcgtctcagcggggg
cccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgttt
ctaatggcttcctccgaagatgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacg
agttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtgg
tccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgac
atcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttg
ttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtc
cgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctctg
aaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggcta
aaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacaccat
cgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa