##gff-version 3 ##sequence-region BBa_J119095 1 1832 BBa_J119095 . mature_transcript_region 764 1832 . + 0 ID=BBa_K199187;Name=BBa_K199187 BBa_J119095 . mature_transcript_region 764 1473 . + 0 ID=BBa_K199181;Name=BBa_K199181;Parent=BBa_K199187 BBa_J119095 . mature_transcript_region 1331 1473 . + 0 ID=BBa_K199014;Name=BBa_K199014;Parent=BBa_K199181 BBa_J119095 . sequence_alteration 1376 1380 . + 0 ID=annotation2007934;Name=5 nt anticodon;Parent=BBa_K199014 BBa_J119095 . promoter 764 963 . + 0 ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K199181 BBa_J119095 . engineered_region 764 963 . + 0 ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010 BBa_J119095 . CDS 764 851 . + 0 ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010 BBa_J119095 . promoter 924 929 . + 0 ID=annotation1961225;Name=-10;Parent=BBa_R0010 BBa_J119095 . non_covalent_binding_site 852 889 . + 0 ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010 BBa_J119095 . non_covalent_binding_site 929 963 . + 0 ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010 BBa_J119095 . promoter 900 905 . + 0 ID=annotation1961224;Name=-35;Parent=BBa_R0010 BBa_J119095 . start_codon 936 936 . + 0 ID=annotation1961227;Name=start;Parent=BBa_R0010 BBa_J119095 . promoter 1123 1322 . + 0 ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K199181 BBa_J119095 . engineered_region 1123 1322 . + 0 ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010 BBa_J119095 . CDS 1123 1210 . + 0 ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010 BBa_J119095 . promoter 1283 1288 . + 0 ID=annotation1961225;Name=-10;Parent=BBa_R0010 BBa_J119095 . non_covalent_binding_site 1211 1248 . + 0 ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010 BBa_J119095 . non_covalent_binding_site 1288 1322 . + 0 ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010 BBa_J119095 . promoter 1259 1264 . + 0 ID=annotation1961224;Name=-35;Parent=BBa_R0010 BBa_J119095 . start_codon 1295 1295 . + 0 ID=annotation1961227;Name=start;Parent=BBa_R0010 BBa_J119095 . mature_transcript_region 972 1114 . + 0 ID=BBa_K199047;Name=BBa_K199047;Parent=BBa_K199181 BBa_J119095 . sequence_feature 1017 1021 . + 0 ID=annotation2011944;Name=5nt anti codon;Parent=BBa_K199047 BBa_J119095 . mature_transcript_region 1482 1832 . + 0 ID=BBa_K199183;Name=BBa_K199183;Parent=BBa_K199187 BBa_J119095 . mature_transcript_region 1690 1832 . + 0 ID=BBa_K199046;Name=BBa_K199046;Parent=BBa_K199183 BBa_J119095 . sequence_feature 1735 1739 . + 0 ID=annotation2011943;Name=5nt anti codon;Parent=BBa_K199046 BBa_J119095 . promoter 1482 1681 . + 0 ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K199183 BBa_J119095 . engineered_region 1482 1681 . + 0 ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010 BBa_J119095 . CDS 1482 1569 . + 0 ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010 BBa_J119095 . promoter 1642 1647 . + 0 ID=annotation1961225;Name=-10;Parent=BBa_R0010 BBa_J119095 . non_covalent_binding_site 1570 1607 . + 0 ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010 BBa_J119095 . non_covalent_binding_site 1647 1681 . + 0 ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010 BBa_J119095 . promoter 1618 1623 . + 0 ID=annotation1961224;Name=-35;Parent=BBa_R0010 BBa_J119095 . start_codon 1654 1654 . + 0 ID=annotation1961227;Name=start;Parent=BBa_R0010 BBa_J119095 . engineered_region 1 755 . + 0 ID=BBa_K199118;Name=BBa_K199118 BBa_J119095 . CDS 50 755 . + 0 ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K199118 BBa_J119095 . CDS 50 724 . + 0 ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010 BBa_J119095 . engineered_tag 731 755 . + 0 ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010 BBa_J119095 . ribosome_entry_site 32 43 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K199118 BBa_J119095 . conserved_region 36 39 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_J119095 . promoter 1 23 . + 0 ID=BBa_R0085;Name=BBa_R0085;Parent=BBa_K199118 BBa_J119095 . sequence_feature 12 23 . + 0 ID=annotation1721522;Name=Initiation Region;Parent=BBa_R0085 BBa_J119095 . non_covalent_binding_site 1 11 . + 0 ID=annotation1721521;Name=Polymerase Binding Region;Parent=BBa_R0085 BBa_J119095 . sequence_feature 18 18 . + 0 ID=annotation1721520;Name=Transcription Start Site;Parent=BBa_R0085 >BBa_J119095 taatacgactcactatagggagatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagag ttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtc cgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtcccc gcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaa ggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacg gtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatggg ttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaa gacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaa ccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtca ctccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagcaatacgcaaaccgcctctccccgcgc gttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgt gagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgga taacaatttcacacatactagagggatccaattcggagagatgccggagcggctgaacggaccggtctattggacaccg gagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttt tttaagcttactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggt ttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttaca ctttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagggatccaattcgg agagatgccggagcggctgaacggaccggtctgtcctaaaccggagtaggggcaactctaccgggggttcaaatccccc tctctccgccactgcatatccttagcgaaagctaaggattttttttaagcttactagagcaatacgcaaaccgcctctc cccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgt gagcggataacaatttcacacatactagagggatccaattcggagagatgccggagcggctgaacggaccggtttagta gataccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaag gattttttttaagct