##gff-version 3
##sequence-region BBa_J119015 1 3585
BBa_J119015	.	engineered_region	2814	3585	.	+	0	ID=BBa_K199176;Name=BBa_K199176
BBa_J119015	.	ribosome_entry_site	2845	2859	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K199176
BBa_J119015	.	ribosome_entry_site	2845	2859	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J119015	.	ribosome_entry_site	2852	2856	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J119015	.	engineered_region	2845	2859	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J119015	.	CDS	2866	3585	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K199176
BBa_J119015	.	CDS	2866	3585	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J119015	.	promoter	2814	2836	.	+	0	ID=BBa_R0085;Name=BBa_R0085;Parent=BBa_K199176
BBa_J119015	.	sequence_feature	2825	2836	.	+	0	ID=annotation1721522;Name=Initiation Region;Parent=BBa_R0085
BBa_J119015	.	non_covalent_binding_site	2814	2824	.	+	0	ID=annotation1721521;Name=Polymerase Binding Region;Parent=BBa_R0085
BBa_J119015	.	sequence_feature	2831	2831	.	+	0	ID=annotation1721520;Name=Transcription Start Site;Parent=BBa_R0085
BBa_J119015	.	CDS	1	2805	.	+	0	ID=BBa_J119009;Name=BBa_J119009
BBa_J119015	.	sequence_feature	2800	2805	.	+	0	ID=annotation2169631;Name=BsmBI;Parent=BBa_J119009
BBa_J119015	.	CDS	89	2788	.	+	0	ID=annotation2169633;Name=T7 RNA Polymerase;Parent=BBa_J119009
BBa_J119015	.	sequence_feature	126	131	.	+	0	ID=annotation2169629;Name=BsaI;Parent=BBa_J119009
BBa_J119015	.	sequence_feature	99	104	.	+	0	ID=annotation2169626;Name=BsaI;Parent=BBa_J119009
BBa_J119015	.	stop_codon	2789	2791	.	+	0	ID=annotation2169632;Name=Stop RNAP;Parent=BBa_J119009
BBa_J119015	.	sequence_feature	107	114	.	+	0	ID=annotation2169627;Name=AsisI;Parent=BBa_J119009
BBa_J119015	.	ribosome_entry_site	68	82	.	+	0	ID=annotation2169624;Name=B0030;Parent=BBa_J119009
BBa_J119015	.	start_codon	89	91	.	+	0	ID=annotation2169625;Name=Start RNAP;Parent=BBa_J119009
BBa_J119015	.	sequence_feature	57	62	.	+	0	ID=annotation2169623;Name=BsmBI;Parent=BBa_J119009
BBa_J119015	.	sequence_feature	137	142	.	+	0	ID=annotation2169630;Name=BbsI;Parent=BBa_J119009
BBa_J119015	.	promoter	1	56	.	+	0	ID=annotation2169622;Name=pLacI;Parent=BBa_J119009
BBa_J119015	.	sequence_feature	117	124	.	+	0	ID=annotation2169628;Name=Asc1;Parent=BBa_J119009
>BBa_J119015
cgttgacaccatcgaatggcgcaaaacctttcgcggtatggcatgatagcgcccggcgtctcggcgaattaaagaggag
aaagtaggcatgaacacgagagaccacgcgatcgcgaggcgcgccaggtctctgctggaagacgtcacgattaacatcg
ctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctagctgaccattacggtgagcgtttagc
tcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaactt
aaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatca
acgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccgga
agccgtagcgtacatcaccattaagaccactctagcttgcctaaccagtgctgacaatacaaccgttcaggctgtagca
agcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaa
acgtcgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgct
ctctaagggtctactcggtggcgaggcgtggtcctcgtggcataaggaggactctattcatgtaggagtacgctgcatc
gagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagacta
tcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggggcgctggctggcatctctccgatgttccaacc
ttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctagcgctg
gtgcgtactcacagtaagaaagcactgatgcgctacgaggacgtttacatgcctgaggtgtacaaagcgattaacattg
cgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggt
cgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaggacatcgacatgaatcctgaggctctcacc
gcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgc
ttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgc
tgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaag
gaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagt
tcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattc
tccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccg
ctggcgtttgacgggtcttgctccggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggtta
acttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgc
aatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggc
actaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggctt
acgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaagggtct
gatgttcactcagccgaatcaggctgctggatacatggctaagctgatttgggaatctgtgagcgtgacggtggtagct
gcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgctgaggtcaaagataagaaaactggagaga
ttcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttccctgtgtggcaggaatacaagaagcctattca
gacgcgcttgaacctgatgttcctcggtcagttccgcttacagcctaccattaacaccaacaaagatagcgagattgat
gcacacaaacaggagtctggtatcgctcctaactttgtacacagccaagacggtagccaccttcgtaagactgtagtgt
gggcacacgagaagtacggaatcgaatcttttgcactgattcacgactccttcggtaccattccggctgacgctgcgaa
cctgttcaaagcagtgcgcgaaactatggttgacacatatgagtcttgtgatgtactggctgatttctacgaccagttc
gctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggctaaaggtaacttgaacctccgtgacatct
tagagtcggacttcgcgttcgcgtaataacgcatgagacgtactagagtaatacgactcactatagggagatactagag
attaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatg
gtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatt
tatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcg
agatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatat
ttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagtt
aaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatac
atcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaac
tagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacaca
atctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattaca
catggcatggatgaactatacaaataataa