##gff-version 3 ##sequence-region BBa_J11022 1 5441 BBa_J11022 . promoter 1 1210 . + 0 ID=BBa_I0500;Name=BBa_I0500 BBa_J11022 . promoter 1043 1114 . + 0 ID=annotation1498842;Name=PC;Parent=BBa_I0500 BBa_J11022 . promoter 1080 1210 . + 0 ID=annotation1498841;Name=Pbad;Parent=BBa_I0500 BBa_J11022 . sequence_feature 1 1079 . + 0 ID=annotation1498843;Name=AraC;Parent=BBa_I0500 BBa_J11022 . terminator 5401 5441 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_J11022 . polyA_site 5428 5441 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_J11022 . stem_loop 5408 5427 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_J11022 . engineered_region 5401 5441 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_J11022 . stop_codon 5434 5434 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_J11022 . CDS 3625 4305 . + 0 ID=BBa_E1010;Name=BBa_E1010 BBa_J11022 . CDS 3625 4299 . + 0 ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010 BBa_J11022 . engineered_tag 4306 4330 . + 0 ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010 BBa_J11022 . CDS 2618 3253 . + 0 ID=BBa_C0056;Name=BBa_C0056 BBa_J11022 . CDS 2618 3253 . + 0 ID=annotation1063931;Name=cI 434;Parent=BBa_C0056 BBa_J11022 . stop_codon 3248 3253 . + 0 ID=annotation1063930;Name=2;Parent=BBa_C0056 BBa_J11022 . terminator 3262 3341 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_J11022 . stem_loop 3273 3316 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_J11022 . engineered_region 3262 3341 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_J11022 . terminator 4427 4467 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_J11022 . polyA_site 4454 4467 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_J11022 . stem_loop 4434 4453 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_J11022 . engineered_region 4427 4467 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_J11022 . stop_codon 4460 4460 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_J11022 . promoter 3399 3598 . + 0 ID=BBa_R0010;Name=BBa_R0010 BBa_J11022 . start_codon 3571 3571 . + 0 ID=annotation1961227;Name=start;Parent=BBa_R0010 BBa_J11022 . non_covalent_binding_site 3487 3524 . + 0 ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010 BBa_J11022 . engineered_region 3399 3598 . + 0 ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010 BBa_J11022 . promoter 3559 3564 . + 0 ID=annotation1961225;Name=-10;Parent=BBa_R0010 BBa_J11022 . non_covalent_binding_site 3564 3598 . + 0 ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010 BBa_J11022 . promoter 3535 3540 . + 0 ID=annotation1961224;Name=-35;Parent=BBa_R0010 BBa_J11022 . CDS 3399 3486 . + 0 ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010 BBa_J11022 . terminator 3350 3390 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_J11022 . polyA_site 3377 3390 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_J11022 . stem_loop 3357 3376 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_J11022 . engineered_region 3350 3390 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_J11022 . stop_codon 3383 3383 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_J11022 . terminator 2486 2526 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_J11022 . polyA_site 2513 2526 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_J11022 . stem_loop 2493 2512 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_J11022 . engineered_region 2486 2526 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_J11022 . stop_codon 2519 2519 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_J11022 . terminator 5313 5392 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_J11022 . stem_loop 5324 5367 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_J11022 . engineered_region 5313 5392 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_J11022 . CDS 4585 5304 . + 0 ID=BBa_E0040;Name=BBa_E0040 BBa_J11022 . CDS 4585 5304 . + 0 ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040 BBa_J11022 . CDS 1237 2389 . + 0 ID=BBa_J06501;Name=BBa_J06501 BBa_J11022 . sequence_alteration 2039 2039 . + 0 ID=annotation1530900;Name=TS mutation (G->A);Parent=BBa_J06501 BBa_J11022 . tag 2326 2364 . + 0 ID=annotation1530907;Name=LVA;Parent=BBa_J06501 BBa_J11022 . engineered_tag 2365 2389 . + 0 ID=annotation1530914;Name=barcode from LacI (BBa_C0012);Parent=BBa_J06501 BBa_J11022 . CDS 1237 2364 . + 0 ID=annotation1530899;Name=LacI(ts)-LVA;Parent=BBa_J06501 BBa_J11022 . ribosome_entry_site 3607 3618 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_J11022 . conserved_region 3611 3614 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_J11022 . ribosome_entry_site 2598 2611 . + 0 ID=BBa_B0031;Name=BBa_B0031 BBa_J11022 . conserved_region 2604 2607 . + 0 ID=annotation23316;Name=conserved;Parent=BBa_B0031 BBa_J11022 . ribosome_entry_site 1219 1230 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_J11022 . conserved_region 1223 1226 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_J11022 . promoter 2535 2588 . + 0 ID=BBa_R0011;Name=BBa_R0011 BBa_J11022 . operator 2537 2553 . + 0 ID=annotation1999;Name=lac O1;Parent=BBa_R0011 BBa_J11022 . engineered_region 2535 2588 . + 0 ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011 BBa_J11022 . promoter 2554 2559 . + 0 ID=annotation2000;Name=-35;Parent=BBa_R0011 BBa_J11022 . operator 2560 2576 . + 0 ID=annotation2001;Name=lac O1;Parent=BBa_R0011 BBa_J11022 . promoter 2577 2582 . + 0 ID=annotation2002;Name=-10;Parent=BBa_R0011 BBa_J11022 . promoter 4476 4557 . + 0 ID=BBa_I12006;Name=BBa_I12006 BBa_J11022 . non_covalent_binding_site 4484 4500 . + 0 ID=annotation786500;Name=OR1 lambda;Parent=BBa_I12006 BBa_J11022 . non_covalent_binding_site 4508 4524 . + 0 ID=annotation786518;Name=OR2 lambda;Parent=BBa_I12006 BBa_J11022 . non_covalent_binding_site 4531 4544 . + 0 ID=annotation786365;Name=OR1 434;Parent=BBa_I12006 BBa_J11022 . promoter 4523 4528 . + 0 ID=annotation837284;Name=-35;Parent=BBa_I12006 BBa_J11022 . promoter 4546 4551 . + 0 ID=annotation837228;Name=-10;Parent=BBa_I12006 BBa_J11022 . terminator 2398 2477 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_J11022 . stem_loop 2409 2452 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_J11022 . engineered_region 2398 2477 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_J11022 . terminator 4339 4418 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_J11022 . stem_loop 4350 4393 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_J11022 . engineered_region 4339 4418 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_J11022 . ribosome_entry_site 4566 4578 . + 0 ID=BBa_B0032;Name=BBa_B0032 BBa_J11022 . engineered_region 4566 4578 . + 0 ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032 BBa_J11022 . ribosome_entry_site 4566 4578 . + 0 ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032 BBa_J11022 . ribosome_entry_site 4572 4575 . + 0 ID=annotation1710;Name=RBS;Parent=BBa_B0032 >BBa_J11022 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac tttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttc tccatacccgtttttttgggctagctactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttat acgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaa aacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaa cagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctc gcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgca caatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagct gcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaag acggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttc tgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaa ggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctgg ttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttgatgcggatatctcggt agtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctg gggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcac tggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagct ggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctga tagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttt tatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatata ctagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagtcacacagga aacctactagatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaa aaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttg cgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttga gcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaag gacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatga cctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagcct tgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaa ggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaa aattcgtataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct gttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactaga gcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaag cgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggc tcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcct ccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcga aggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttc gcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacc tgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttaccca ggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggtt atgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatca aaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttca gctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtac gaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggca tcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactag agtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcaaccattatcaccgccagaggtaaaa tagtcaacacgcacggtgttagatattacaaactttcttgtatagatttaacgttactagagtcacacaggaaagtact agatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaa attttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaa ctacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatga aacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaa ctacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaa gaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaa agaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaaca aaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagat cccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactat acaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata