##gff-version 3
##sequence-region BBa_J11020 1 3679
BBa_J11020	.	terminator	806	885	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J11020	.	engineered_region	806	885	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J11020	.	stem_loop	817	860	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J11020	.	terminator	3502	3581	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J11020	.	engineered_region	3502	3581	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J11020	.	stem_loop	3513	3556	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J11020	.	ribosome_entry_site	1033	1047	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J11020	.	engineered_region	1033	1047	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J11020	.	ribosome_entry_site	1040	1044	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J11020	.	ribosome_entry_site	1033	1047	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J11020	.	CDS	1054	1803	.	+	0	ID=BBa_C0081;Name=BBa_C0081
BBa_J11020	.	CDS	1054	1764	.	+	0	ID=annotation308222;Name=cI 857;Parent=BBa_C0081
BBa_J11020	.	tag	1765	1797	.	+	0	ID=annotation308236;Name=LVA;Parent=BBa_C0081
BBa_J11020	.	promoter	1	49	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_J11020	.	promoter	38	43	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_J11020	.	engineered_region	1	49	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_J11020	.	non_covalent_binding_site	25	41	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_J11020	.	promoter	15	20	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_J11020	.	non_covalent_binding_site	1	17	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_J11020	.	promoter	943	1024	.	+	0	ID=BBa_I12007;Name=BBa_I12007
BBa_J11020	.	non_covalent_binding_site	975	991	.	+	0	ID=annotation937702;Name=OR2;Parent=BBa_I12007
BBa_J11020	.	non_covalent_binding_site	951	967	.	+	0	ID=annotation937701;Name=OR1;Parent=BBa_I12007
BBa_J11020	.	promoter	1013	1018	.	+	0	ID=annotation937705;Name=-10;Parent=BBa_I12007
BBa_J11020	.	sequence_alteration	1001	1007	.	+	0	ID=annotation937704;Name=mutate to obliterate OR3;Parent=BBa_I12007
BBa_J11020	.	promoter	990	995	.	+	0	ID=annotation937703;Name=-35;Parent=BBa_I12007
BBa_J11020	.	ribosome_entry_site	58	71	.	+	0	ID=BBa_B0031;Name=BBa_B0031
BBa_J11020	.	conserved_region	64	67	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_J11020	.	terminator	3639	3679	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J11020	.	stem_loop	3646	3665	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J11020	.	polyA_site	3666	3679	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J11020	.	engineered_region	3639	3679	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J11020	.	stop_codon	3672	3672	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J11020	.	CDS	2744	3493	.	+	0	ID=BBa_C0081;Name=BBa_C0081
BBa_J11020	.	CDS	2744	3454	.	+	0	ID=annotation308222;Name=cI 857;Parent=BBa_C0081
BBa_J11020	.	tag	3455	3487	.	+	0	ID=annotation308236;Name=LVA;Parent=BBa_C0081
BBa_J11020	.	terminator	894	934	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J11020	.	stem_loop	901	920	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J11020	.	polyA_site	921	934	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J11020	.	engineered_region	894	934	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J11020	.	stop_codon	927	927	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J11020	.	terminator	2612	2652	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J11020	.	stem_loop	2619	2638	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J11020	.	polyA_site	2639	2652	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J11020	.	engineered_region	2612	2652	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J11020	.	stop_codon	2645	2645	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J11020	.	terminator	2524	2603	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J11020	.	engineered_region	2524	2603	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J11020	.	stem_loop	2535	2578	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J11020	.	terminator	3590	3630	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J11020	.	stem_loop	3597	3616	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J11020	.	polyA_site	3617	3630	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J11020	.	engineered_region	3590	3630	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J11020	.	stop_codon	3623	3623	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J11020	.	CDS	1810	2490	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_J11020	.	CDS	1810	2484	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_J11020	.	engineered_tag	2491	2515	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_J11020	.	CDS	78	797	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_J11020	.	CDS	78	797	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J11020	.	ribosome_entry_site	2723	2737	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J11020	.	engineered_region	2723	2737	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J11020	.	ribosome_entry_site	2730	2734	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J11020	.	ribosome_entry_site	2723	2737	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J11020	.	promoter	2661	2714	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J11020	.	non_covalent_binding_site	2661	2679	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J11020	.	engineered_region	2661	2714	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J11020	.	promoter	2680	2685	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J11020	.	promoter	2703	2708	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J11020	.	non_covalent_binding_site	2686	2704	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
>BBa_J11020
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagtcacacaggaaacctactagat
gcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattt
tctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactac
ctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaaca
gcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactac
aagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaag
atggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaa
tggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaat
actccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatccca
acgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaa
ataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt
gtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcaacc
attatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgttac
tagagattaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcg
ccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatgggg
cagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctca
aagttagcgttgaagaatttagcccttcaatcgccacagaaatctacgagatgtatgaagcggttagtatgcagccgtc
acttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctaagcttagaacctttacc
aaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaatt
ccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttga
gccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtg
tttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatggcta
gtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataatactagatggcttc
ctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatc
gaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgt
tcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggacta
cctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacc
caggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccgg
ttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaat
caaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggtt
cagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagt
acgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccagg
catcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctact
agagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacat
ccctatcagtgatagagatactgagcactactagagattaaagaggagaaatactagatgagcacaaaaaagaaaccat
taacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatccca
ggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgct
tataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccacagaaatctacg
agatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagg
gatgttctcacctaagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgat
tctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgt
taattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttacctt
caagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgag
agttgttccgttgtggggaaagttatggctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacg
ctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttta
tctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatact
agagtcacactggctcaccttcgggtgggcctttctgcgtttata