##gff-version 3
##sequence-region BBa_J107026 1 2852
BBa_J107026	.	engineered_region	908	2852	.	+	0	ID=BBa_T9002;Name=BBa_T9002
BBa_J107026	.	ribosome_entry_site	1977	1989	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_T9002
BBa_J107026	.	ribosome_entry_site	1977	1989	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J107026	.	engineered_region	1977	1989	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J107026	.	ribosome_entry_site	1983	1986	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J107026	.	terminator	1777	1856	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_T9002
BBa_J107026	.	engineered_region	1777	1856	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J107026	.	stem_loop	1788	1831	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J107026	.	terminator	2812	2852	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_T9002
BBa_J107026	.	engineered_region	2812	2852	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J107026	.	stem_loop	2819	2838	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J107026	.	polyA_site	2839	2852	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J107026	.	stop_codon	2845	2845	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J107026	.	terminator	2724	2803	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_T9002
BBa_J107026	.	engineered_region	2724	2803	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J107026	.	stem_loop	2735	2778	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J107026	.	terminator	1865	1905	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_T9002
BBa_J107026	.	engineered_region	1865	1905	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J107026	.	stem_loop	1872	1891	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J107026	.	polyA_site	1892	1905	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J107026	.	stop_codon	1898	1898	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J107026	.	CDS	988	1743	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_T9002
BBa_J107026	.	engineered_region	988	989	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_J107026	.	engineered_region	988	1743	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_J107026	.	engineered_tag	1744	1768	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_J107026	.	point_mutation	1479	1479	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_J107026	.	point_mutation	1161	1161	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_J107026	.	CDS	988	1737	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_J107026	.	ribosome_entry_site	970	981	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_T9002
BBa_J107026	.	conserved_region	974	977	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J107026	.	promoter	1914	1968	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_T9002
BBa_J107026	.	non_covalent_binding_site	1914	1933	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J107026	.	promoter	1933	1938	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J107026	.	engineered_region	1914	1968	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J107026	.	promoter	1955	1960	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J107026	.	start_codon	1966	1966	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J107026	.	promoter	908	961	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_T9002
BBa_J107026	.	promoter	950	955	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J107026	.	promoter	927	932	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J107026	.	engineered_region	908	961	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J107026	.	non_covalent_binding_site	933	951	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J107026	.	non_covalent_binding_site	908	926	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J107026	.	CDS	1996	2715	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_T9002
BBa_J107026	.	CDS	1996	2715	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J107026	.	engineered_region	1	899	.	+	0	ID=BBa_J107023;Name=BBa_J107023
BBa_J107026	.	engineered_region	39	899	.	+	0	ID=BBa_I13507;Name=BBa_I13507;Parent=BBa_J107023
BBa_J107026	.	CDS	57	737	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_J107026	.	CDS	57	731	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_J107026	.	engineered_tag	738	762	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_J107026	.	terminator	859	899	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_J107026	.	engineered_region	859	899	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J107026	.	stem_loop	866	885	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J107026	.	polyA_site	886	899	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J107026	.	stop_codon	892	892	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J107026	.	ribosome_entry_site	39	50	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_J107026	.	conserved_region	43	46	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J107026	.	terminator	771	850	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_J107026	.	engineered_region	771	850	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J107026	.	stem_loop	782	825	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J107026	.	promoter	1	30	.	+	0	ID=BBa_R0061;Name=BBa_R0061;Parent=BBa_J107023
BBa_J107026	.	promoter	1	6	.	+	0	ID=annotation305863;Name=-35;Parent=BBa_R0061
BBa_J107026	.	promoter	25	30	.	+	0	ID=annotation305864;Name=-10;Parent=BBa_R0061
BBa_J107026	.	non_covalent_binding_site	6	25	.	+	0	ID=annotation305877;Name=Lux Box;Parent=BBa_R0061
>BBa_J107026
ttgacacctgtaggatcgtacaggtataattactagagaaagaggagaaatactagatggcttcctccgaagacgttat
caaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaa
ggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcc
tgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtcctt
cccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctg
caagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaa
ccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaa
actgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgct
tacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaag
gtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatag
agatactgagcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataatta
ataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaata
ttatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatgg
aggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaatta
attggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttat
cactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaac
tatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaa
taaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaa
aagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaa
ctcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaatt
aataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgg
gcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttct
gcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactaga
gtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatgg
tgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaattt
atttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcga
gatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatt
tttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagtta
aaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtataca
tcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaact
agcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaa
tctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacac
atggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttata