##gff-version 3
##sequence-region BBa_J107016 1 1845
BBa_J107016	.	engineered_region	927	1845	.	+	0	ID=BBa_K173002;Name=BBa_K173002
BBa_J107016	.	terminator	1805	1845	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K173002
BBa_J107016	.	engineered_region	1805	1845	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J107016	.	stop_codon	1838	1838	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J107016	.	polyA_site	1832	1845	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J107016	.	stem_loop	1812	1831	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J107016	.	ribosome_entry_site	970	982	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K173002
BBa_J107016	.	ribosome_entry_site	970	982	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J107016	.	engineered_region	970	982	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J107016	.	ribosome_entry_site	976	979	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J107016	.	CDS	989	1708	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K173002
BBa_J107016	.	CDS	989	1708	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J107016	.	terminator	1717	1796	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K173002
BBa_J107016	.	engineered_region	1717	1796	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J107016	.	stem_loop	1728	1771	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J107016	.	promoter	927	961	.	+	0	ID=BBa_J23118;Name=BBa_J23118;Parent=BBa_K173002
BBa_J107016	.	engineered_region	1	918	.	+	0	ID=BBa_I763007;Name=BBa_I763007
BBa_J107016	.	promoter	1	49	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_I763007
BBa_J107016	.	engineered_region	1	49	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_J107016	.	promoter	15	20	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_J107016	.	non_covalent_binding_site	1	17	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_J107016	.	promoter	38	43	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_J107016	.	non_covalent_binding_site	25	41	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_J107016	.	engineered_region	58	918	.	+	0	ID=BBa_I13507;Name=BBa_I13507;Parent=BBa_I763007
BBa_J107016	.	ribosome_entry_site	58	69	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_J107016	.	conserved_region	62	65	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J107016	.	terminator	790	869	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_J107016	.	engineered_region	790	869	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J107016	.	stem_loop	801	844	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J107016	.	CDS	76	756	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_J107016	.	CDS	76	750	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_J107016	.	engineered_tag	757	781	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_J107016	.	terminator	878	918	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_J107016	.	engineered_region	878	918	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J107016	.	stop_codon	911	911	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J107016	.	polyA_site	905	918	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J107016	.	stem_loop	885	904	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
>BBa_J107016
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatgg
cttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcga
aatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctg
ccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccgg
actacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgt
tacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggt
ccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtg
aaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaacc
ggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaa
cagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagc
caggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
tactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtcctag
gtattgtgctagctactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtccca
attcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacg
gaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggtta
tggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttat
gtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgataccc
ttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaacta
taactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacatt
gaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagaca
accattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgt
aacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaa
ggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctca
ccttcgggtgggcctttctgcgtttata