LOCUS       BBa_J107016             1845 bp    DNA     linear       10-Dec-2025
DEFINITION  Constitutive generator of RFP (R0051) and GFP (J23118)
ACCESSION   BBa_J107016
VERSION     BBa_J107016.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             76..750
                     /label=mrfp1
     misc_feature    757..781
                     /label=Help:Barcodes
     CDS             76..756
                     /label=BBa_E1010
     stem_loop       801..844
                     /label=stem_loop
     misc_feature    790..869
                     /label=BBa_B0010
     terminator      790..869
                     /label=BBa_B0010
     stem_loop       885..904
                     /label=T7 TE
     misc_feature    911..911
                     /label=stop
     polyA_site      905..918
                     /label=polya
     misc_feature    878..918
                     /label=BBa_B0012
     terminator      878..918
                     /label=BBa_B0012
     misc_feature    58..918
                     /label=BBa_I13507
     misc_feature    1..918
                     /label=BBa_I763007
     promoter        927..961
                     /label=BBa_J23118
     RBS             970..982
                     /label=RBS-3\Weak
     RBS             976..979
                     /label=RBS
     misc_feature    970..982
                     /label=BBa_B0032
     RBS             970..982
                     /label=BBa_B0032
     CDS             989..1708
                     /label=GFP protein
     CDS             989..1708
                     /label=BBa_E0040
     stem_loop       1728..1771
                     /label=stem_loop
     misc_feature    1717..1796
                     /label=BBa_B0010
     terminator      1717..1796
                     /label=BBa_B0010
     stem_loop       1812..1831
                     /label=T7 TE
     misc_feature    1838..1838
                     /label=stop
     polyA_site      1832..1845
                     /label=polya
     misc_feature    1805..1845
                     /label=BBa_B0012
     terminator      1805..1845
                     /label=BBa_B0012
     misc_feature    927..1845
                     /label=BBa_K173002
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa
      121 gttcgtatgg aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt
      181 cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg
      241 ttcgcttggg acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac
      301 ccggctgaca tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt
      361 gttatgaact tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac
      421 ggtgagttca tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt
      481 atgcagaaaa aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt
      541 gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct
      601 gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc
      661 gacatcaaac tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa
      721 cgtgctgaag gtcgtcactc caccggtgct taataacgct gatagtgcta gtgtagatcg
      781 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
      841 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
      901 ggcctttctg cgtttatata ctagagttga cggctagctc agtcctaggt attgtgctag
      961 ctactagagt cacacaggaa agtactagat gcgtaaagga gaagaacttt tcactggagt
     1021 tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg
     1081 agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg
     1141 aaaactacct gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt
     1201 tgcgagatac ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg
     1261 ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga
     1321 agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa
     1381 agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata
     1441 catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat
     1501 tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg
     1561 ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc
     1621 caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca
     1681 tggcatggat gaactataca aataataata ctagagccag gcatcaaata aaacgaaagg
     1741 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     1801 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//