##gff-version 3
##sequence-region BBa_J100307 1 2414
BBa_J100307	.	promoter	728	763	.	+	0	ID=annotation2488208;Name=P5 Promoter
BBa_J100307	.	sequence_feature	1577	1637	.	+	0	ID=annotation2488215;Name=Terminator L3S2P21
BBa_J100307	.	ribosome_entry_site	1490	1501	.	+	0	ID=annotation2488212;Name=B0034
BBa_J100307	.	sequence_alteration	243	243	.	+	0	ID=annotation2488206;Name=T->G
BBa_J100307	.	sequence_alteration	953	953	.	+	0	ID=annotation2488211;Name=T->C
BBa_J100307	.	sequence_feature	1517	1522	.	+	0	ID=annotation2488213;Name=BsaI Recognition Site
BBa_J100307	.	promoter	1523	1568	.	+	0	ID=annotation2488214;Name=P2 Promoter
BBa_J100307	.	sequence_feature	1638	1643	.	+	0	ID=annotation2488216;Name=BsaI Recognition Site
BBa_J100307	.	CDS	1734	2414	.	+	0	ID=annotation2488218;Name=RFP
BBa_J100307	.	CDS	764	1483	.	+	0	ID=annotation2488209;Name=GFP Optimized Sequence
BBa_J100307	.	CDS	1	639	.	+	0	ID=annotation2488205;Name=tetR Repressor C0041
BBa_J100307	.	sequence_alteration	945	945	.	+	0	ID=annotation2488210;Name=T->C
BBa_J100307	.	ribosome_entry_site	640	727	.	+	0	ID=annotation2488207;Name=BD24
BBa_J100307	.	ribosome_entry_site	1649	1736	.	+	0	ID=annotation2488217;Name=BD18
>BBa_J100307
cggccgctactagtattattagctaccgctttcacatttcagttgtttttccagtccgcaaataatcagttctaaacca
aacagaaaggcaggttctgcaccctgatgatcaaacagttcaatggcctgacgcagcagcggaggcatgctatcggtgg
tcggggtttcgcgttcttcttttgcaacctgatgttcttgatcttccagaacacaacccagggtaaaatgaccaactgc
gctcagtgcatacagtgcgttttccagactaaaaccttgctgacacagaaatgccagctgattttccagggtttcatac
tgtttttcggtcggacgggtgcccagatgaacttttgcaccatcacgatggctcagcagggcacaacgaaagctttttg
cattattacgcagaaaatcctgccagctttcaccttccagcggacaaaaatgggtatgatgacgatccagcatttcaat
tgccagtgcatccagcagtgcacgtttgtttttaacatgccaatacagggtcggctgttcaacacccagtttctgtgcc
agtttacgggtggtcagaccttcaataccaacttcattcagcagttccagtgcgctattaatcactttgcttttatcca
gacggctcattagaaaccgtccatcgcatgattaagatgtttcagtacgaaaattgctttcattgttgatctccttttt
aagtgaacttgggccctccacataaattacgagccggatgattaattgtcaattattatttgtatagttcatccatgcc
atgtgtaatcccagcagctgttacaaactcaagaaggaccatgtgatctctctcttcgttgggatctttcgaaagggca
gattgtgtggacaggtaatggttgtctggtaaaaggacagggccatcgccaattggagtattttgttgataatggcctg
ctagctgaacgcttccatcttcaatgttgtgtctaattttgaagttaactttgattccattcttttgtttgtctgccat
gatgtatacattgtgtgagttatagttgtattccaatttgtgtccaagaatatttccatcttctttaaaatcaatacct
tttaactcgattctattaacaagggtatcaccttcaaacttgacttcagcacgtgtcttgtagttcccgtcatctttga
aaaatacagttctttcctgtacataaccttcgggcatggcactcttgaaaaagtcatgctgtttcatatgatctgggta
tctcgcaaagcattgaacaccataaccgaaagtagtgacaagtgttggccatggaacaggtagttttccagtagtgcaa
ataaatttaagggtaagttttccgtatgttgcatcaccttcaccctctccactgacagaaaatttgtgcccattaacat
cgccatctaattcaacaagaattgggacaactccagtgaaaagttcttctcctttacgcacctagtatttctcctcttt
aattactagacgacagagacctccacacattataggtacaaaaagatgcgaagtcaatactctttttggccaattctcg
gtaccaaattccagaaaagaggcctcccgaaaggggggccttttttcgttttggtccggtctctgcgggggcccaagtt
cacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgtttctaatggc
ttcctccgaagatgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaa
atcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgc
cgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccgga
ctacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgtt
acccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtc
cggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctctgaaaggtga
aatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccg
gttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacaccatcgttgaac
agtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa