##gff-version 3
##sequence-region BBa_J100253 1 1663
BBa_J100253	.	ribosome_entry_site	727	738	.	+	0	ID=annotation2478615;Name=B0030 RBS reverse
BBa_J100253	.	CDS	896	1663	.	+	0	ID=annotation2478619;Name=mCherry 
BBa_J100253	.	sequence_feature	755	758	.	+	0	ID=annotation2478620;Name=Sticky end
BBa_J100253	.	sequence_feature	795	798	.	+	0	ID=annotation2478617;Name=Sticky end
BBa_J100253	.	promoter	759	794	.	+	0	ID=annotation2478616;Name=P5
BBa_J100253	.	CDS	1	720	.	+	0	ID=annotation2478614;Name=GFP reverse
BBa_J100253	.	ribosome_entry_site	811	895	.	+	0	ID=annotation2478618;Name=BD18 bicistroin RBS
>BBa_J100253
ttattatttgtatagttcatccatgccatgtgtaatcccagcagctgttacaaactcaagaaggaccatgtgatctctc
ttttcgttgggatctttcgaaagggcagattgtgtggacaggtaatggttgtctggtaaaaggacagggccatcgccaa
ttggagtattttgttgataatggtctgctagttgaacgcttccatcttcaatgttgtgtctaattttgaagttaacttt
gattccattcttttgtttgtctgccatgatgtatacattgtgtgagttatagttgtattccaatttgtgtccaagaatg
tttccatcttctttaaaatcaataccttttaactcgattctattaacaagggtatcaccttcaaacttgacttcagcac
gtgtcttgtagttcccgtcatctttgaaaaatatagttctttcctgtacataaccttcgggcatggcactcttgaaaaa
gtcatgctgtttcatatgatctgggtatctcgcaaagcattgaacaccataaccgaaagtagtgacaagtgttggccat
ggaacaggtagttttccagtagtgcaaataaatttaagggtaagttttccgtatgttgcatcaccttcaccctctccac
tgacagaaaatttgtgcccattaacatcaccatctaattcaacaagaattgggacaactccagtgaaaagttcttctcc
tttacgcatctagtatttctcctctttaatactagaggtcttccgacttgacaattaatcatccggctcgtaatttatg
tggagcgggaagacaactaggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacat
cttaatcatgcgacggagcgtttctaatgggatcctcgcatcatcatcaccaccattcctcaggtttagtccctcgcgg
gagccatatggtatcgaaaggggaagaggacaatatggcaattatcaaggagttcatgcgttttaaggttcatatggag
gggagtgtaaacggccacgaattcgagatcgaaggtgaaggggaagggcgtccatatgagggtacacagactgctaagt
taaaggtcacgaagggcggccctttacctttcgcatgggatattctgtcgccccagtttatgtatggctcgaaggctta
tgtaaaacacccggcggatattcctgactatttaaaactgtcattcccagaaggttttaagtgggagcgcgtcatgaac
ttcgaggatggaggcgtcgtaacagttacccaggattcatcgttgcaagacggggagttcatttacaaagttaaacttc
gtggcacgaactttcctagtgacggacctgtaatgcaaaagaagaccatggggtgggaggcttcctcggagcgcatgta
ccccgaggacggcgctttgaaaggggagatcaaacaacgcttgaaattgaaagatggagggcattatgatgcagaggtg
aaaaccacatacaaggctaagaagccagtgcaactgccgggtgcatacaatgttaatatcaaactggatattacctctc
acaatgaggactatacgatcgtcgaacagtatgagcgcgccgaaggacgccattccacaggcggtatggatgaacttta
caagtaataa