LOCUS       BBa_J100204             1744 bp    DNA     linear       28-Jun-2025
DEFINITION  actClone Red
ACCESSION   BBa_J100204
VERSION     BBa_J100204.1  
FEATURES             Location/Qualifiers
     misc_feature    1..6
                     /label=EcoRI
     CDS             7..726
                     /label=superfolding GFP
     RBS             726..811
                     /label=RBS BD24
     misc_feature    817..822
                     /label=BsaI site
     promoter        823..858
                     /label=P5 Promoter
     misc_feature    867..927
                     /label=Terminator L3S2P21
     misc_feature    928..933
                     /label=BsaI site
     promoter        935..972
                     /label=3' half, ompC promoter
     RBS             973..1058
                     /label=RBS BD21
     CDS             1058..1738
                     /label=RFP
     misc_feature    1739..1744
                     /label=PstI site
ORIGIN
        1 gaattctcat catttgtaca gttcatccat accatgcgtg atgcccgctg cggttacgaa
       61 ctccagcaga accatatgat cgcgtttctc gttcggatct ttagacagaa cgctttgcgt
      121 gctcagatag tgattgtctg gcagcagaac aggaccatca ccgattggag tgttttgctg
      181 gtagtgatca gccagctgca cgctgccatc ctccacgttg tggcgaattt taaaattcgc
      241 tttaatgcca tttttttgtt tatcggcggt gatgtaaaca ttgtggctgt taaaattgta
      301 ttccagctta tggcccagga tattgccgtc ttctttaaag tcaatgcctt tcagctcaat
      361 gcggtttacc agggtatcgc cttcaaattt cacttccgca cgcgttttgt acgtgccgtc
      421 atccttaaag gaaatcgtgc gttcctgcac atagccttcc ggcatggcgg acttgaagaa
      481 gtcatgctgc ttcatatggt ccggataacg agcaaagcac tgaacaccat aagtcagcgt
      541 cgttaccaga gtcggccaag gtaccggcag tttaccagta gtacagatga acttcagcgt
      601 cagtttacca ttagttgcgt caccttcacc ctcgccacgc acggaaaact tatgaccgtt
      661 gacatcacca tccagttcca ccagaatagg gacgacacca gtgaacagct cttcgccttt
      721 acgcattaga aaccgtccat cgcatgatta agatgtttca gtacgaaaat tgctttcatt
      781 gttgatctcc tttttaagtg aacttgggcc ccgacagaga cctccacata aattacgagc
      841 cggatgatta attgtcaagg ccaattctcg gtaccaaatt ccagaaaaga ggcctcccga
      901 aaggggggcc ttttttcgtt ttggtccggt ctcacgtgtt ggattattct gcatttttgg
      961 ggagaatgga ctgggcccaa gttcacttaa aaaggagatc aacaatgaaa gcaattttcg
     1021 tactgaaaca tcttaatcat gcgagggatg gtttctaatg gcttcctccg aagacgttat
     1081 caaagagttc atgcgtttca aagttcgtat ggaaggttcc gttaacggtc acgagttcga
     1141 aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt
     1201 taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt tccagtacgg
     1261 ttccaaagct tacgttaaac acccggctga catcccggac tacctgaaac tgtccttccc
     1321 ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac
     1381 ccaggactcc tccctgcaag acggtgagtt catctacaaa gttaaactgc gtggtaccaa
     1441 cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag cttccaccga
     1501 acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa
     1561 agacggtggt cactacgacg ctgaagttaa aaccacctac atggctaaaa aaccggttca
     1621 gctgccgggt gcttacaaaa ccgacatcaa actggacatc acctcccaca acgaagacta
     1681 caccatcgtt gaacagtacg aacgtgctga aggtcgtcac tccaccggtg cttaataact
     1741 gcag
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