##gff-version 3 ##sequence-region BBa_J100106 1 4182 BBa_J100106 . ribosome_entry_site 146 149 . + 0 ID=annotation2214366;Name=RBS BBa_J100106 . engineered_region 888 4182 . + 0 ID=annotation2214383;Name=BBa_J100105 BBa_J100106 . engineered_region 888 1011 . + 0 ID=annotation2214372;Name=BBa_S05031 BBa_J100106 . engineered_region 4162 4182 . + 0 ID=annotation2214382;Name=Bba_suffix BBa_J100106 . engineered_region 1 879 . + 0 ID=annotation2214369;Name=BBa_J100079 BBa_J100106 . sequence_feature 82 96 . + 0 ID=annotation2214363;Name=constant sequence BBa_J100106 . engineered_region 888 923 . + 0 ID=annotation2214371;Name=BBa_J119030 BBa_J100106 . stop_codon 4156 4161 . + 0 ID=annotation2214381;Name=Double Stop BBa_J100106 . engineered_region 1012 4182 . + 0 ID=annotation2214380;Name=J100100 BBa_J100106 . sequence_feature 102 162 . + 0 ID=annotation2214365;Name=Riboswitch BBa_J100106 . operator 54 74 . + 0 ID=annotation2214361;Name=lac operator BBa_J100106 . start_codon 956 958 . + 0 ID=annotation2214376;Name=Start BBa_J100106 . engineered_region 924 1011 . + 0 ID=annotation2214374;Name=BBa_J119028 BBa_J100106 . ribosome_entry_site 942 950 . + 0 ID=annotation2214375;Name=leader RBS BBa_J100106 . start_codon 1009 1011 . + 0 ID=annotation2214379;Name=Start BBa_J100106 . sequence_feature 97 102 . + 0 ID=annotation2214364;Name=KpnI site BBa_J100106 . stop_codon 1007 1009 . + 0 ID=annotation2214378;Name=Stop BBa_J100106 . promoter 1 53 . + 0 ID=annotation2214360;Name=T5 promoter BBa_J100106 . sequence_feature 888 893 . + 0 ID=annotation2214370;Name=-35 BBa_J100106 . CDS 160 879 . + 0 ID=annotation2214368;Name=superfolder GFP BBa_J100106 . start_codon 160 162 . + 0 ID=annotation2214367;Name=Start BBa_J100106 . sequence_feature 75 80 . + 0 ID=annotation2214362;Name=scar BBa_J100106 . ribosome_entry_site 994 1002 . + 0 ID=annotation2214377;Name=GOI RBS BBa_J100106 . sequence_feature 911 923 . + 0 ID=annotation2214373;Name=-10 >BBa_J100106 aaatcataaaaaatttatttgctttgtgagcggataacaattataatagattcaattgtgagcggataacaattactag agatacgactcactataggtaccggtgataccagcatcgtcttgatgcccttggcagcaccctgctaaggtaacaacaa gatgcgtaaaggcgaagagctgttcaccggtgttgttccgattctggttgaactggatggtgatgttaatggccacaaa ttttcagttcgtggtgaaggcgagggtgatgcaaccaatggtaaactgaccctgaaatttatctgtaccaccggcaaac tgccggttccgtggccgaccctggttaccaccctgacctatggtgttcagtgttttgcacgttatccggatcatatgaa acagcacgattttttcaaaagcgcaatgccggaaggttatgttcaagaacgtaccatctcctttaaagatgatggcacc tataaaacccgtgccgaagttaaatttgaaggtgacaccctggtgaatcgtattgagctgaaaggcatcgatttcaaag aggatggtaatatcctgggccataaactggaatataacttcaatagccacaatgtgtatatcaccgcagacaaacagaa aaacggcattaaagccaactttaagattcgccataatgtggaagatggtagcgtgcagctggcagatcattatcagcag aataccccgattggtgatggtccggttctgctgccggataatcactatctgagcacccagagcgttctgagcaaagatc cgaatgaaaaacgtgatcacatggtgctgctggaatttgttaccgcagcaggtattacccatggtatggatgaactgta caaatgatgatactagagttgacatcaggaaaatttttctgtataatgtgtggagggcccaagttcacttaaaaaggag atcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgatggacggtttctaatgaccatcaaagaaatgc cgcagccgaaaacctttggtgaactgaaaaatctgccgctgctgaataccgataaaccggttcatgcactgatgaaaat tgcagatgaactgggcgagatctttaaattcgaagcaccgggtagcgttacccgttatctgagcagccagcgtctgatt aaagaagcatgcgacgaaagccgctttgataaaaatctgattcagtggctgaaattcatccgcgattttctgggtgatg gtctgagcaccagctggacccatgaaaaaaactggaaaaaagcccataatattctgctgccgagctttagccagcaggc aatgaaaggttatcatgcaatgatggttgatattgccgttcagctggttcagaaatgggaacgtctgaatgcagatgaa catattgaagttccggaagatatgacccgtctgaccctggataccattggtctgtgtggttttaactatcgcttcaaca gcttttatcgtgatcagccgcatccgtttgttaccagcatggttcgtgcactggatgaagcagtgaataaatggcagcg tgcaaatccggatgatccggcatatgatgaaaataaacgtcagttccaagaggatattaaagtgatgaatgacctggtg gacaaaattatcgcagatcgtaaagcaagcggtgaacagagtgatgatctgctgacccatatgctgaatggtaaagatc cggaaaccggtgaaccgctggatgatgaaaacattcgttatcagattattaccttcctgattgccggtcatgaaaccac cagtggtctgctgagctttgcactgtattttctggttaaaaatccgcacgttctgcaaaaagcagccgaagaagcagca cgcgttctggttgatccggttccgagctataaacaggttaaacagctgaaatatgtgggcatggttctgaatgaagcac tgcgtctgtggccgacctttccgtggtttagcctgtatgcaaaagaagataccgttctgggaggtgaatatccgctgga aaaaggtgatgaactgatggttctgattccgcagctgcatcgtgataaaaccatttggggtgatgatgtggaagaattt cgtccggaacgttttgaaaatccgagcgcaattccgcagcatgcctttaaaccgtttggtaatggtcagcgtgcatgta ttggtcagcagtttgcactgcatgaagccaccctggttctgggtatgatgctgaaacattttgattttgaggaccacac caactatgagctggatattaaaggcaccctgaccctgaaaccggaaggttttgttgttaaagccaaaagcaaaaaaatc ccgctgggtggtattccgagcccgagcaccgaacagagcgcaaaaaaagttcgtaaaaaagccgaaaacgcccataata caccgctgctggttctgtatggtagcaatatgggtacagccgaaggcaccgcacgtgatctggcagatattgcaatgag caaaggttttgcaccgcaggttgcaaccctggatagccatgcaggtaatctgcctcgtgaaggtgcagttctgattgtt accgcaagctataatggtcatccgcctgataatgcaaaacagtttgttgattggctggatcaggcaagcgcagatgaag ttaaaggtgttcgttatagcgtttttggttgcggtgataaaaactgggcaaccacctatcagaaagttccggcatttat tgatgaaaccctggcagcaaaaggtgcagaaaatattgcagatcgtggtgaaaccgatgccagtgatgattttgaaggc acctatgaagaatggcgtgaacatatgtggtcagatgttgcagcctattttaacctggatatcgaaaacagcgaggaca ataaaagcaccctgagcctgcaatttgtggatagcgcagcagatatgccgctggcaaaaatgcatggtgcatttagcac caatgttgttgcaagcaaagaactgcaacagcctggtagcgcacgtagcacccgtcatctggaaattgaactgccgaaa gaagcaagctatcaagagggtgatcatctgggtgttattccgcgtaattatgaaggtattgttaatcgtgttaccgcac gttttggtctggatgcaagccagcagattcgtctggaagcagaagaagaaaaactggcacatctgccgctggccaaaac cgttagcgttgaagaactgctgcaatatgttgaactgcaagatccggttacccgtacccagctgcgtgcaatggcagca aaaaccgtttgtccgcctcataaagttgaactggaagcactgctggagaaacaggcatataaagaacaggttctggcaa aacgtctgaccatgctggaactgctggaaaaatatccggcatgcgaaatgaaattcagcgaatttattgcactgctgcc gagtattcgtccgcgttattatagcattagcagcagtccgcgtgttgatgaaaaacaggccagcattaccgttagcgtg gttagcggtgaagcatggtcaggttatggtgaatataaaggtattgccagcaattatctggccgaactgcaagaaggtg ataccattacctgttttattagcacaccgcagagcgaatttaccctgccgaaagatcctgaaacaccgctgattatggt tggtccgggtacaggtgttgcaccgtttcgtggttttgttcaggcacgtaaacaactgaaagaacagggtcagagcctg ggtgaagcacatctgtattttggttgtcgtagtccgcatgaggattatctgtatcaagaagaactggaaaacgcacaga gcgaaggtattattaccctgcataccgcatttagccgtatgccgaatcagccgaaaacatatgttcagcatgttatgga acaggatggcaaaaaactgattgaactgctggatcagggtgcccatttctatatttgtggtgatggtagccagatggca ccggcagttgaagcaaccctgatgaaaagctatgcagatgttcatcaggttagcgaagcagatgcacgtctgtggctgc aacagctggaagaaaaaggtcgttatgcaaaagatgtttgggcaggttaataatactagtagcggccgctgcag