##gff-version 3
##sequence-region BBa_J100105 1 3295
BBa_J100105	.	engineered_region	3275	3295	.	+	0	ID=annotation2214181;Name=Bba_suffix
BBa_J100105	.	sequence_feature	1	6	.	+	0	ID=annotation2214171;Name=-35
BBa_J100105	.	stop_codon	120	122	.	+	0	ID=annotation2214177;Name=Stop
BBa_J100105	.	engineered_region	1	124	.	+	0	ID=annotation2214179;Name=BBa_S05031
BBa_J100105	.	stop_codon	3269	3274	.	+	0	ID=annotation2214182;Name=Double Stop
BBa_J100105	.	engineered_region	125	3295	.	+	0	ID=annotation2214180;Name=J100100
BBa_J100105	.	engineered_region	37	124	.	+	0	ID=annotation2214173;Name=BBa_J119028
BBa_J100105	.	start_codon	69	71	.	+	0	ID=annotation2214175;Name=Start
BBa_J100105	.	sequence_feature	24	36	.	+	0	ID=annotation2214172;Name=-10
BBa_J100105	.	ribosome_entry_site	107	115	.	+	0	ID=annotation2214176;Name=GOI RBS
BBa_J100105	.	start_codon	122	124	.	+	0	ID=annotation2214178;Name=Start
BBa_J100105	.	engineered_region	1	36	.	+	0	ID=annotation2214170;Name=BBa_J119030
BBa_J100105	.	ribosome_entry_site	55	63	.	+	0	ID=annotation2214174;Name=leader RBS
>BBa_J100105
ttgacatcaggaaaatttttctgtataatgtgtggagggcccaagttcacttaaaaaggagatcaacaatgaaagcaat
tttcgtactgaaacatcttaatcatgcgatggacggtttctaatgaccatcaaagaaatgccgcagccgaaaacctttg
gtgaactgaaaaatctgccgctgctgaataccgataaaccggttcatgcactgatgaaaattgcagatgaactgggcga
gatctttaaattcgaagcaccgggtagcgttacccgttatctgagcagccagcgtctgattaaagaagcatgcgacgaa
agccgctttgataaaaatctgattcagtggctgaaattcatccgcgattttctgggtgatggtctgagcaccagctgga
cccatgaaaaaaactggaaaaaagcccataatattctgctgccgagctttagccagcaggcaatgaaaggttatcatgc
aatgatggttgatattgccgttcagctggttcagaaatgggaacgtctgaatgcagatgaacatattgaagttccggaa
gatatgacccgtctgaccctggataccattggtctgtgtggttttaactatcgcttcaacagcttttatcgtgatcagc
cgcatccgtttgttaccagcatggttcgtgcactggatgaagcagtgaataaatggcagcgtgcaaatccggatgatcc
ggcatatgatgaaaataaacgtcagttccaagaggatattaaagtgatgaatgacctggtggacaaaattatcgcagat
cgtaaagcaagcggtgaacagagtgatgatctgctgacccatatgctgaatggtaaagatccggaaaccggtgaaccgc
tggatgatgaaaacattcgttatcagattattaccttcctgattgccggtcatgaaaccaccagtggtctgctgagctt
tgcactgtattttctggttaaaaatccgcacgttctgcaaaaagcagccgaagaagcagcacgcgttctggttgatccg
gttccgagctataaacaggttaaacagctgaaatatgtgggcatggttctgaatgaagcactgcgtctgtggccgacct
ttccgtggtttagcctgtatgcaaaagaagataccgttctgggaggtgaatatccgctggaaaaaggtgatgaactgat
ggttctgattccgcagctgcatcgtgataaaaccatttggggtgatgatgtggaagaatttcgtccggaacgttttgaa
aatccgagcgcaattccgcagcatgcctttaaaccgtttggtaatggtcagcgtgcatgtattggtcagcagtttgcac
tgcatgaagccaccctggttctgggtatgatgctgaaacattttgattttgaggaccacaccaactatgagctggatat
taaaggcaccctgaccctgaaaccggaaggttttgttgttaaagccaaaagcaaaaaaatcccgctgggtggtattccg
agcccgagcaccgaacagagcgcaaaaaaagttcgtaaaaaagccgaaaacgcccataatacaccgctgctggttctgt
atggtagcaatatgggtacagccgaaggcaccgcacgtgatctggcagatattgcaatgagcaaaggttttgcaccgca
ggttgcaaccctggatagccatgcaggtaatctgcctcgtgaaggtgcagttctgattgttaccgcaagctataatggt
catccgcctgataatgcaaaacagtttgttgattggctggatcaggcaagcgcagatgaagttaaaggtgttcgttata
gcgtttttggttgcggtgataaaaactgggcaaccacctatcagaaagttccggcatttattgatgaaaccctggcagc
aaaaggtgcagaaaatattgcagatcgtggtgaaaccgatgccagtgatgattttgaaggcacctatgaagaatggcgt
gaacatatgtggtcagatgttgcagcctattttaacctggatatcgaaaacagcgaggacaataaaagcaccctgagcc
tgcaatttgtggatagcgcagcagatatgccgctggcaaaaatgcatggtgcatttagcaccaatgttgttgcaagcaa
agaactgcaacagcctggtagcgcacgtagcacccgtcatctggaaattgaactgccgaaagaagcaagctatcaagag
ggtgatcatctgggtgttattccgcgtaattatgaaggtattgttaatcgtgttaccgcacgttttggtctggatgcaa
gccagcagattcgtctggaagcagaagaagaaaaactggcacatctgccgctggccaaaaccgttagcgttgaagaact
gctgcaatatgttgaactgcaagatccggttacccgtacccagctgcgtgcaatggcagcaaaaaccgtttgtccgcct
cataaagttgaactggaagcactgctggagaaacaggcatataaagaacaggttctggcaaaacgtctgaccatgctgg
aactgctggaaaaatatccggcatgcgaaatgaaattcagcgaatttattgcactgctgccgagtattcgtccgcgtta
ttatagcattagcagcagtccgcgtgttgatgaaaaacaggccagcattaccgttagcgtggttagcggtgaagcatgg
tcaggttatggtgaatataaaggtattgccagcaattatctggccgaactgcaagaaggtgataccattacctgtttta
ttagcacaccgcagagcgaatttaccctgccgaaagatcctgaaacaccgctgattatggttggtccgggtacaggtgt
tgcaccgtttcgtggttttgttcaggcacgtaaacaactgaaagaacagggtcagagcctgggtgaagcacatctgtat
tttggttgtcgtagtccgcatgaggattatctgtatcaagaagaactggaaaacgcacagagcgaaggtattattaccc
tgcataccgcatttagccgtatgccgaatcagccgaaaacatatgttcagcatgttatggaacaggatggcaaaaaact
gattgaactgctggatcagggtgcccatttctatatttgtggtgatggtagccagatggcaccggcagttgaagcaacc
ctgatgaaaagctatgcagatgttcatcaggttagcgaagcagatgcacgtctgtggctgcaacagctggaagaaaaag
gtcgttatgcaaaagatgtttgggcaggttaataatactagtagcggccgctgcag