##gff-version 3
##sequence-region BBa_J100015 1 2757
BBa_J100015	.	CDS	219	856	.	+	0	ID=BBa_J100013;Name=BBa_J100013
BBa_J100015	.	start_codon	219	221	.	+	0	ID=annotation2122238;Name=Start;Parent=BBa_J100013
BBa_J100015	.	stop_codon	851	856	.	+	0	ID=annotation2122242;Name=Stop;Parent=BBa_J100013
BBa_J100015	.	sequence_feature	231	241	.	+	0	ID=annotation2122240;Name=SAT Problem;Parent=BBa_J100013
BBa_J100015	.	sequence_feature	222	229	.	+	0	ID=annotation2122239;Name=AsiSI Site;Parent=BBa_J100013
BBa_J100015	.	sequence_feature	246	253	.	+	0	ID=annotation2122241;Name=AscI Site;Parent=BBa_J100013
BBa_J100015	.	terminator	2629	2757	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J100015	.	terminator	2629	2708	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J100015	.	stem_loop	2640	2683	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J100015	.	engineered_region	2629	2708	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J100015	.	terminator	2717	2757	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J100015	.	stem_loop	2724	2743	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J100015	.	stop_codon	2750	2750	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J100015	.	polyA_site	2744	2757	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J100015	.	engineered_region	2717	2757	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J100015	.	promoter	1614	1813	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_J100015	.	non_covalent_binding_site	1702	1739	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J100015	.	promoter	1774	1779	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J100015	.	non_covalent_binding_site	1779	1813	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J100015	.	promoter	1750	1755	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J100015	.	start_codon	1786	1786	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J100015	.	engineered_region	1614	1813	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J100015	.	CDS	1614	1701	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J100015	.	ribosome_entry_site	201	212	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J100015	.	conserved_region	205	208	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J100015	.	ribosome_entry_site	1822	1833	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J100015	.	conserved_region	1826	1829	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J100015	.	promoter	138	192	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_J100015	.	non_covalent_binding_site	138	157	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J100015	.	promoter	157	162	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J100015	.	promoter	179	184	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J100015	.	engineered_region	138	192	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J100015	.	start_codon	190	190	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J100015	.	ribosome_entry_site	865	879	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J100015	.	ribosome_entry_site	865	879	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J100015	.	engineered_region	865	879	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J100015	.	ribosome_entry_site	872	876	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J100015	.	CDS	886	1605	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_J100015	.	CDS	886	1605	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J100015	.	CDS	1840	2595	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_J100015	.	engineered_region	1840	1841	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_J100015	.	engineered_tag	2596	2620	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_J100015	.	point_mutation	2331	2331	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_J100015	.	engineered_region	1840	2595	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_J100015	.	point_mutation	2013	2013	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_J100015	.	CDS	1840	2589	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_J100015	.	terminator	1	129	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J100015	.	terminator	1	80	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J100015	.	stem_loop	12	55	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J100015	.	engineered_region	1	80	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J100015	.	terminator	89	129	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J100015	.	stem_loop	96	115	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J100015	.	stop_codon	122	122	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J100015	.	polyA_site	116	129	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J100015	.	engineered_region	89	129	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_J100015
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtt
tacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatggcgatcgcaccactgc
tagttctgggcgcgccgcaattgcaagcggtcgtgcaaccattatgattaaaaaaagcgattttctggccattccgagc
gaagaatataaaggtattctgagcctgcgctatcaggtttttaaacagcgtctggaatgggatctggtggttgaaaata
atctggaaagtgatgaatatgataatagcaatgccgaatatatttatgcctgtgatgataccgaaaatgttagcggttg
ttggcgtctgctgccgaccaccggtgattatatgctgaaaagcgtttttccggaactgctgggtcagcagagcgcaccg
aaagatccgaatattgttgaactgagccgttttgccgtgggtaaaaatagcagcaaaattaataatagcgccagcgaaa
ttaccatgaaactgtttgaagccatttataaacatgccgttagccagggtattaccgaatatgttaccgttaccagcac
cgcaattgaacgttttctgaaacgtattaaagtgccgtgtcatcgtattggcgataaagaaattcatgttctgggcgat
accaaaagcgttgttctgagcatgccgattaatgaacagtttaaaaaagccgtgctgaactaataatactagagattaa
agaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgat
gttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttattt
gcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagata
cccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttc
aaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaag
gtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcat
ggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagca
gaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctg
ccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatgg
catggatgaactatacaaataataatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatg
cagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattag
gcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatact
agagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgt
agaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatca
tttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatga
cgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaa
aacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttcc
ctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatt
tttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataat
aaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatattt
caaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccg
ctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgc
tagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata