##gff-version 3
##sequence-region BBa_J07086 1 4874
BBa_J07086	.	promoter	1	74	.	+	0	ID=BBa_J13002;Name=BBa_J13002
BBa_J07086	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J13002
BBa_J07086	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J07086	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J07086	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J07086	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J07086	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J07086	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J13002
BBa_J07086	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J07086	.	sequence_feature	3206	3754	.	+	0	ID=BBa_J07068;Name=BBa_J07068
BBa_J07086	.	CDS	3227	3754	.	+	0	ID=BBa_J07022;Name=BBa_J07022;Parent=BBa_J07068
BBa_J07086	.	CDS	3227	3754	.	+	0	ID=annotation1577017;Name=FecI;Parent=BBa_J07022
BBa_J07086	.	ribosome_entry_site	3206	3220	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_J07068
BBa_J07086	.	engineered_region	3206	3220	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J07086	.	ribosome_entry_site	3213	3217	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J07086	.	ribosome_entry_site	3206	3220	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J07086	.	sequence_feature	3763	4874	.	+	0	ID=BBa_J07072;Name=BBa_J07072
BBa_J07086	.	terminator	4834	4874	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J07072
BBa_J07086	.	stem_loop	4841	4860	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J07086	.	engineered_region	4834	4874	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J07086	.	stop_codon	4867	4867	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J07086	.	polyA_site	4861	4874	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J07086	.	terminator	4746	4825	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J07072
BBa_J07086	.	engineered_region	4746	4825	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J07086	.	stem_loop	4757	4800	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J07086	.	CDS	3784	4737	.	+	0	ID=BBa_J07023;Name=BBa_J07023;Parent=BBa_J07072
BBa_J07086	.	CDS	3784	4737	.	+	0	ID=annotation1577975;Name=FecR;Parent=BBa_J07023
BBa_J07086	.	ribosome_entry_site	3763	3777	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_J07072
BBa_J07086	.	engineered_region	3763	3777	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J07086	.	ribosome_entry_site	3770	3774	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J07086	.	ribosome_entry_site	3763	3777	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J07086	.	CDS	81	3197	.	+	0	ID=BBa_J07063;Name=BBa_J07063
>BBa_J07086
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgacgccgttacgcgtttttcgtaaaacaacacctttggttaacaccattcgcctgagcctgctgccgctggccggt
ctctcgttttccgcttttgctgcacaggttaatatcgcaccgggatcgctcgataaagcgctcaatcagtatgccgcac
acagcggatttaccctctcggttgacgccagcctgacgcgcggcaagcagagcaacggcctgcacggcgattacgacgt
cgagagcggcctgcaacaactgctggacggcagcggactgcaagtaaaaccgctgggaaataacagctggacgctggag
cccgcgcccgcaccaaaagaagatgccctgaccgtggtcggcgactggctgggtgatgcgcgtgaaaacgacgtatttg
aacatgctggcgcgcgtgacgtgatccgccgtgaggatttcgccaaaaccggcgcaaccaccatgcgtgaggtacttaa
ccgcatccctggcgtcagcgcgccggaaaacaacggcaccggcagccacgacctggcgatgaactttggcatccggggc
ctgaacccgcgcctcgccagccgctcgaccgtcctgatggacggcatccccgtccccttcgccccttacggtcagccgc
agctttcactggctcccgtttcgctcggcaacatggatgccattgacgtggtacgcggtggtggtgcggtgcgttacgg
accgcagagcgtgggcggcgtggtgaactttgttacccgtgccattccgcaggactttggtatcgaggcgggcgtggaa
ggtcagctcagcccaacctcttcacaaaacaacccgaaagagacgcacaacctgatggtgggcggcacagcggacaacg
gttttggcaccgcgctgctctactccggcacgcgcggcagtgactggcgcgagcacagcgccacccgcatcgacgacct
gatgctgaaaagcaaatatgcgccggatgaggtgcacaccttcaacagcctgctgcaatattacgacggtgaagccgac
atgcccggtggcctgtctcgcgcggattacgacgccgatcgctggcaatccacccgcccgtatgaccgcttctggggtc
gtcgcaagctggcgagcctgggctaccagttccagccagacagccagcataaattcaacattcaggggttctacaccca
aaccctgcgcagcggctacctggagcaaggcaaacgcatcaccctctcgccgcgtaactactgggtgcgcggtattgag
ccacgctacagccagatctttatgatcggcccttccgcgcacgaagtgggcgtgggctatcgctatttgaatgaatcaa
cgcatgaaatgcgttactacaccgccaccagcagcgggcagttgccgtccggctcaagcccttacgaccgcgatacgcg
ttccggcaccgaggcgcacgcctggtatctggatgacaaaatcgacatcggcaactggaccatcacgccgggtatgcgt
ttcgaacatatcgagtcataccagaacaacgccatcacaggcacgcacgaagaagtgagctataacgcaccgcttccgg
cgttgaacgtgctctatcacctgactgacagctggaatctttatgcaaacactgaaggctcgttcggcaccgtacagta
cagccagattggcaaggctgtgcaaagcggcaatgttgaagacgtcgttatgactcaaacaccactatcacttcctgtt
agtctaggtgatcaagcctccatctcttgcagatctagtcagagccttgtacacagtaatggaaacacctatttacgtt
ggtacctgcaaaagccaggccagtctccaaaggtcctgatctacaaagtttccaaccgagtttctggggtcccagacag
gttcagtggcagtggatcagggacagatttcacactcaagatcaacagagtggaggctgaggatctgggagtttatttc
tgctctcaaagtacacatgttccgtggacgttcggtggaggcaccaagcttgaaattaagtcctctgctgatgatgcta
agaaggatgctgctaagaaggatgatgctaagaaagatgatgctaagaaagatggtggcgtcaaactggatgagactgg
aggaggcttggtgcaacctgggggggccatgaaactctcctgtgttacctctggattcacttttggtcactactggatg
aactgggtccgccagtctccagagaaaggactggagtgggtagcacaatttagaaacaaaccttataattatgaaacat
attattcagattctgtgaaaggcagattcaccatctcaagagatgattccaaaagtagtgtctacctgcaaatgaacaa
cttaagagttgaagacaccggtatctattactgtacgggtgcgtcttatggtatggaatacttaggtcaaggaacctca
gtcaccgtctccccgaacagcgcgagccatagcggcagcgcgccgaacaccagcagcgcgccgggcagcacccgctacg
acgacggcgcgctgacggcggaaatggggctgttcctgattaactttaacaatcagtacgactccaaccagaccaacga
caccgtcactgcacgtggcaaaacgcgccataccgggctggaaacgcaggcacgttacgatctgggtacgctaacgcca
acgcttgataacgtttccatctacgccagctatgcgtatgtgaacgcggaaatccgcgagaaaggcgacacctacggca
atctggtaccattctccccgaaacataaaggcacgctgggcgtggactacaagccaggaaactggacgttcaatctgaa
cagcgatttccagtccagccagtttgcggataacgccaatacggtgaaagagagcgccgacggcagtaccggccgcatt
cccggcttcatgctctggggcgcacgcgtggcgtatgactttggcccgcagatggcagatctgaacctggcattcggtg
tgaaaaacatcttcgaccaggactacttcatccgctcttatgacgacaacaacaaaggcatctatgcaggccagccgcg
cacgctgtatatgcaggggtcgttgaagttctaataatactagagattaaagaggagaaatactagatgtctgaccgcg
ccactaccacagcttccttaacgttcgagtcgctttatggcacacatcacggctggttgaaaagctggctgacgcgcaa
actccagtctgcttttgatgcagatgacattgcccaggacacttttttgcgggtaatggtcagcgaaacgctctcgacg
atccgcgatcctcgctccttcctctgcactatcgccaaacgcgtgatggtggacctgtttcgccgaaacgcgctggaaa
aagcgtatctggagatgctggcgcttatgccggaggggggagcgccttcacctgaggaacgcgaaagccaactcgagac
cctacaactcctcgacagcatgctggacgggctaaacggcaaaacacgtgaagcgtttctgctttcgcaactggatggt
ctgacatacagcgagattgcgcacaaactcggtgtttccatcagctccgtgaaaaaatacgtggcgaaagccgtcgagc
actgcctgctgttccgtctggagtatgggttatgataataatactagagattaaagaggagaaatactagatgaatcct
ttgttaaccgattcccgccgtcaggcgctgcgttcagcttcccactggtatgccgtgctaagcggcgagcgcgtcagcc
cacaacaggaagcgcgctggcaacagtggtatgaacaggatcaggataaccagtgggcctggcagcaggttgaaaacct
gcgcaaccagcttggcggtgtgcctggcgacgttgccagccgggcgttgcacgatacccgcctcacccgccgtcacgtg
atgaaaggattactgctgttgctcggcgctggcggaggctggcagctctggcagtcggaaaccggcgaaggtctgcggg
cagattaccgcaccgccaaaggcacagtcagccgtcagcaactggaagatggctccctgctcacgctgaatacccaaag
cgcggcggatgtgcgttttgatgcgcatcagcgcaccgtccggctctggtacggtgaaatcgccattactaccgcgaaa
gatgccctgcaacgccccttccgtgtcctgacccgtcagggccagctcactgctttagggacagaatttaccgtccgcc
agcaggataatttcacgcagcttgacgtgcagcagcacgctgtggaagtgcttctcgccagtgcccccgcgcaaaaacg
catcgtgaacgctggtgaaagcctacagttcagcgcctctgagtttggcgcagtgaaaccgctggatgacgagagtaca
agctggacgaaggacatcctgagcttcagcgataaaccgctgggtgaggtgatagccacgctaacccgttaccgcaacg
gcgtgctgcgctgcgatcccgccgttgccgggctgcgcctgagcgggacgttcccgctgaaaaataccgatgcgatcct
gaacgttatcgcgcaaacgcttcccgttaaaattcagtctattacgcggtactggataaacatttcaccactgtaatac
tagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaac
gctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata