##gff-version 3 ##sequence-region BBa_J07080 1 1617 BBa_J07080 . terminator 1577 1617 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_J07080 . polyA_site 1604 1617 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_J07080 . stem_loop 1584 1603 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_J07080 . engineered_region 1577 1617 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_J07080 . stop_codon 1610 1610 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_J07080 . CDS 81 710 . + 0 ID=BBa_J07009;Name=BBa_J07009 BBa_J07080 . sequence_feature 81 626 . + 0 ID=annotation1668506;Name=Cytoplasmic Region;Parent=BBa_J07009 BBa_J07080 . sequence_feature 675 710 . + 0 ID=annotation1668510;Name=Periplasmic Region;Parent=BBa_J07009 BBa_J07080 . start_codon 86 88 . + 0 ID=annotation1668508;Name=Start Codon;Parent=BBa_J07009 BBa_J07080 . sequence_feature 627 674 . + 0 ID=annotation1668509;Name=Transmembrane Region;Parent=BBa_J07009 BBa_J07080 . CDS 81 710 . + 0 ID=annotation1668507;Name=Coding Sequence for ToxR';Parent=BBa_J07009 BBa_J07080 . ribosome_entry_site 63 74 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_J07080 . conserved_region 67 70 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_J07080 . terminator 1489 1568 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_J07080 . stem_loop 1500 1543 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_J07080 . engineered_region 1489 1568 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_J07080 . engineered_region 719 1480 . + 0 ID=BBa_J07014;Name=BBa_J07014 BBa_J07080 . sequence_feature 1055 1129 . + 0 ID=annotation1582325;Name=linker;Parent=BBa_J07014 BBa_J07080 . sequence_feature 1130 1480 . + 0 ID=annotation1582336;Name=VH;Parent=BBa_J07014 BBa_J07080 . sequence_alteration 1130 1132 . + 0 ID=annotation1583279;Name=D-G;Parent=BBa_J07014 BBa_J07080 . sequence_alteration 1280 1282 . + 0 ID=annotation1583294;Name=I-F;Parent=BBa_J07014 BBa_J07080 . sequence_feature 1429 1450 . + 0 ID=annotation1582331;Name=CDR3 VH;Parent=BBa_J07014 BBa_J07080 . sequence_alteration 1178 1180 . + 0 ID=annotation1583287;Name=P-A;Parent=BBa_J07014 BBa_J07080 . sequence_feature 1220 1231 . + 0 ID=annotation1582328;Name=CDR1 VH;Parent=BBa_J07014 BBa_J07080 . sequence_alteration 1430 1432 . + 0 ID=annotation1582343;Name=S-A;Parent=BBa_J07014 BBa_J07080 . sequence_feature 779 838 . + 0 ID=annotation1582322;Name=CDR1 VL;Parent=BBa_J07014 BBa_J07080 . sequence_alteration 1430 1432 . + 0 ID=annotation1583296;Name=S-A;Parent=BBa_J07014 BBa_J07080 . sequence_alteration 959 961 . + 0 ID=annotation1583278;Name=S-N;Parent=BBa_J07014 BBa_J07080 . sequence_alteration 1451 1453 . + 0 ID=annotation1583299;Name=W-L;Parent=BBa_J07014 BBa_J07080 . sequence_alteration 1406 1408 . + 0 ID=annotation1583295;Name=M-T;Parent=BBa_J07014 BBa_J07080 . sequence_alteration 1199 1201 . + 0 ID=annotation1583288;Name=A-T;Parent=BBa_J07014 BBa_J07080 . sequence_alteration 1217 1219 . + 0 ID=annotation1583292;Name=S-G;Parent=BBa_J07014 BBa_J07080 . sequence_alteration 1175 1177 . + 0 ID=annotation1583280;Name=R-G;Parent=BBa_J07014 BBa_J07080 . sequence_alteration 1445 1447 . + 0 ID=annotation1583298;Name=D-E;Parent=BBa_J07014 BBa_J07080 . sequence_feature 719 1054 . + 0 ID=annotation1582335;Name=VL;Parent=BBa_J07014 BBa_J07080 . sequence_feature 1277 1333 . + 0 ID=annotation1582330;Name=CDR2 VH;Parent=BBa_J07014 BBa_J07080 . sequence_feature 998 1024 . + 0 ID=annotation1582324;Name=CDR3 VL;Parent=BBa_J07014 BBa_J07080 . sequence_alteration 1433 1435 . + 0 ID=annotation1583297;Name=Y-S;Parent=BBa_J07014 BBa_J07080 . sequence_alteration 1220 1222 . + 0 ID=annotation1583293;Name=D-H;Parent=BBa_J07014 BBa_J07080 . sequence_alteration 896 898 . + 0 ID=annotation1583271;Name=F-V;Parent=BBa_J07014 BBa_J07080 . sequence_feature 881 901 . + 0 ID=annotation1582323;Name=CDR2 VL;Parent=BBa_J07014 BBa_J07080 . promoter 1 54 . + 0 ID=BBa_R0040;Name=BBa_R0040 BBa_J07080 . promoter 20 25 . + 0 ID=annotation1986785;Name=-35;Parent=BBa_R0040 BBa_J07080 . promoter 43 48 . + 0 ID=annotation1986787;Name=-10;Parent=BBa_R0040 BBa_J07080 . non_covalent_binding_site 1 19 . + 0 ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040 BBa_J07080 . engineered_region 1 54 . + 0 ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040 BBa_J07080 . non_covalent_binding_site 26 44 . + 0 ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040 >BBa_J07080 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta gatgttcggattaggacacaactcaaaagagatatcgatgagtcatattggtactaaattcattcttgctgaaaaattt accttcgatcccctaagcaatactctgattgacaaagaagatagtgaagagatcattcgattaggcagcaacgaaagcc gaatcctttggctgctggcccaacgtccaaacgaggtaatttctcgcaatgatttgcatgactttgtttggcgagagca aggttttgaagtcgatgattccagcttaacccaagccatttcgactctgcgcaaaatgctcaaagattcgacaaagtcc ccacaatacgtcaaaacggttccgaagcgcggttaccaattgatcgcccgagtggaaacggttgaagaagagatggctc gcgaaaacgaagctgctcatgacatctctcagccagaatctgtcaatgaatacgcagaatcaagcagtgtgccttcatc agccactgtagtgaacacaccgcagccagccaatgtcgtggcgaataaatcggctccaaacttggggaatcgactgttt attctgatagcggtcttacttcccctcgcagtattactgctcactaacccaagccaatccagctttaaacccctaacgt actagaggacgtcgttatgactcaaacaccactatcacttcctgttagtctaggtgatcaagcctccatctcttgcaga tctagtcagagccttgtacacagtaatggaaacacctatttacgttggtacctgcaaaagccaggccagtctccaaagg tcctgatctacaaagtttccaaccgagtttctggggtcccagacaggttcagtggcagtggatcagggacagatttcac actcaagatcaacagagtggaggctgaggatctgggagtttatttctgctctcaaagtacacatgttccgtggacgttc ggtggaggcaccaagcttgaaattaagtcctctgctgatgatgctaagaaggatgctgctaagaaggatgatgctaaga aagatgatgctaagaaagatggtggcgtcaaactggatgagactggaggaggcttggtgcaacctgggggggccatgaa actctcctgtgttacctctggattcacttttggtcactactggatgaactgggtccgccagtctccagagaaaggactg gagtgggtagcacaatttagaaacaaaccttataattatgaaacatattattcagattctgtgaaaggcagattcacca tctcaagagatgattccaaaagtagtgtctacctgcaaatgaacaacttaagagttgaagacaccggtatctattactg tacgggtgcgtcttatggtatggaatacttaggtcaaggaacctcagtcaccgtctcctactagagccaggcatcaaat aaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcac actggctcaccttcgggtgggcctttctgcgtttata