##gff-version 3
##sequence-region BBa_J07027 1 2274
BBa_J07027	.	terminator	2234	2274	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J07027	.	stem_loop	2241	2260	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J07027	.	polyA_site	2261	2274	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J07027	.	stop_codon	2267	2267	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J07027	.	engineered_region	2234	2274	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J07027	.	CDS	84	713	.	+	0	ID=BBa_J07009;Name=BBa_J07009
BBa_J07027	.	sequence_feature	84	629	.	+	0	ID=annotation1668506;Name=Cytoplasmic Region;Parent=BBa_J07009
BBa_J07027	.	start_codon	89	91	.	+	0	ID=annotation1668508;Name=Start Codon;Parent=BBa_J07009
BBa_J07027	.	CDS	84	713	.	+	0	ID=annotation1668507;Name=Coding Sequence for ToxR';Parent=BBa_J07009
BBa_J07027	.	sequence_feature	630	677	.	+	0	ID=annotation1668509;Name=Transmembrane Region;Parent=BBa_J07009
BBa_J07027	.	sequence_feature	678	713	.	+	0	ID=annotation1668510;Name=Periplasmic Region;Parent=BBa_J07009
BBa_J07027	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J07027	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J07027	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J07027	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J07027	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J07027	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J07027	.	ribosome_entry_site	63	77	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J07027	.	ribosome_entry_site	63	77	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J07027	.	ribosome_entry_site	70	74	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J07027	.	engineered_region	63	77	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J07027	.	terminator	2146	2225	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J07027	.	engineered_region	2146	2225	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J07027	.	stem_loop	2157	2200	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J07027	.	CDS	722	2137	.	+	0	ID=BBa_J07075;Name=BBa_J07075
>BBa_J07027
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaata
ctagatgttcggattaggacacaactcaaaagagatatcgatgagtcatattggtactaaattcattcttgctgaaaaa
tttaccttcgatcccctaagcaatactctgattgacaaagaagatagtgaagagatcattcgattaggcagcaacgaaa
gccgaatcctttggctgctggcccaacgtccaaacgaggtaatttctcgcaatgatttgcatgactttgtttggcgaga
gcaaggttttgaagtcgatgattccagcttaacccaagccatttcgactctgcgcaaaatgctcaaagattcgacaaag
tccccacaatacgtcaaaacggttccgaagcgcggttaccaattgatcgcccgagtggaaacggttgaagaagagatgg
ctcgcgaaaacgaagctgctcatgacatctctcagccagaatctgtcaatgaatacgcagaatcaagcagtgtgccttc
atcagccactgtagtgaacacaccgcagccagccaatgtcgtggcgaataaatcggctccaaacttggggaatcgactg
tttattctgatagcggtcttacttcccctcgcagtattactgctcactaacccaagccaatccagctttaaacccctaa
cgtactagaggtgaaacaaagcactattgcactggcactcttaccgttactgtttacccctgtgacaaaagcccggaca
ccagaaatgcctgttctggaaaaccgggctgctcagggcgatattactgcacccggcggtgctcgccgtttaacgggtg
atcagactgccgctctgcgtgattctcttagcgataaacctgcaaaaaatattattttgctgattggcgatgggatggg
ggactcggaaattactgccgcacgtaattatgccgaaggtgcgggcggcttttttaaaggtatagatgccttaccgctt
accgggcaatacactcactatgcgctgaataaaaaaaccggcaaaccggactacgtcaccgactcggctgcatcagcaa
ccgcctggtcaaccggtgtcaaaacctataacggcgcgctgggcgtcgatattcacgaaaaagatcacccaacgattct
ggaaatggcaaaagccgcaggtctggcgaccggtaacgtttctaccgcagagttgcaggatgccacgcccgctgcgctg
gtggcacatgtgacctcgcgcaaatgctacggtccgagcgcgaccagtgaaaaatgtccgggtaacgctctggaaaaag
gcggaaaaggatcgattaccgaacagctgcttaacgctcgtgccgacgttacgcttggcggcggcgcaaaaacctttgc
tgaaacggcaaccgctggtgaatggcagggaaaaacgctgcgtgaacaggcacaggcgcgtggttatcagttggtgagc
gatgctgcctcactgaactcggtgacggaagcgaatcagcaaaaacccctgcttggcctgtttgctgacggcaatatgc
cagtgcgctggctaggaccgaaagcaacgtaccatggcaatatcgataagcccgcagtcacctgtacgccaaatccgca
acgtaatgacagtgtaccaaccctggcgcagatgaccgacaaagccattgaattgttgagtaaaaatgagaaaggcttt
ttcctgcaagttgaaggtgcgtcaatcgataaacaggatcatgctgcgaatccttgtgggcaaattggcgagacggtcg
atctcgatgaagccgtacaacgggcgctggagttcgctaaaaaggagggtaacacgctggtcatagtcaccgctgatca
cgcccacgccagccagattgttgcgccggataccaaagctccgggcctcacccaggcgctaaataccaaagatggcgca
gtgatggtgatgagttacgggaactccgaagaggattcacaagaacataccggcagtcagttgcgtattgcggcgtatg
gcccgcatgccgccaatgttgttggactgaccgaccagaccgatctcttctacaccatgaaagccgctctggggctgaa
ataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata