##gff-version 3
##sequence-region BBa_J07026 1 1620
BBa_J07026	.	engineered_region	722	1483	.	+	0	ID=BBa_J07014;Name=BBa_J07014
BBa_J07026	.	sequence_alteration	1202	1204	.	+	0	ID=annotation1583288;Name=A-T;Parent=BBa_J07014
BBa_J07026	.	sequence_alteration	1454	1456	.	+	0	ID=annotation1583299;Name=W-L;Parent=BBa_J07014
BBa_J07026	.	sequence_alteration	1133	1135	.	+	0	ID=annotation1583279;Name=D-G;Parent=BBa_J07014
BBa_J07026	.	sequence_alteration	1181	1183	.	+	0	ID=annotation1583287;Name=P-A;Parent=BBa_J07014
BBa_J07026	.	sequence_alteration	1433	1435	.	+	0	ID=annotation1583296;Name=S-A;Parent=BBa_J07014
BBa_J07026	.	sequence_feature	1058	1132	.	+	0	ID=annotation1582325;Name=linker;Parent=BBa_J07014
BBa_J07026	.	sequence_feature	1133	1483	.	+	0	ID=annotation1582336;Name=VH;Parent=BBa_J07014
BBa_J07026	.	sequence_alteration	899	901	.	+	0	ID=annotation1583271;Name=F-V;Parent=BBa_J07014
BBa_J07026	.	sequence_feature	1432	1453	.	+	0	ID=annotation1582331;Name=CDR3 VH;Parent=BBa_J07014
BBa_J07026	.	sequence_alteration	962	964	.	+	0	ID=annotation1583278;Name=S-N;Parent=BBa_J07014
BBa_J07026	.	sequence_feature	782	841	.	+	0	ID=annotation1582322;Name=CDR1 VL;Parent=BBa_J07014
BBa_J07026	.	sequence_alteration	1409	1411	.	+	0	ID=annotation1583295;Name=M-T;Parent=BBa_J07014
BBa_J07026	.	sequence_feature	1280	1336	.	+	0	ID=annotation1582330;Name=CDR2 VH;Parent=BBa_J07014
BBa_J07026	.	sequence_alteration	1220	1222	.	+	0	ID=annotation1583292;Name=S-G;Parent=BBa_J07014
BBa_J07026	.	sequence_alteration	1433	1435	.	+	0	ID=annotation1582343;Name=S-A;Parent=BBa_J07014
BBa_J07026	.	sequence_alteration	1283	1285	.	+	0	ID=annotation1583294;Name=I-F;Parent=BBa_J07014
BBa_J07026	.	sequence_feature	722	1057	.	+	0	ID=annotation1582335;Name=VL;Parent=BBa_J07014
BBa_J07026	.	sequence_feature	884	904	.	+	0	ID=annotation1582323;Name=CDR2 VL;Parent=BBa_J07014
BBa_J07026	.	sequence_alteration	1436	1438	.	+	0	ID=annotation1583297;Name=Y-S;Parent=BBa_J07014
BBa_J07026	.	sequence_alteration	1178	1180	.	+	0	ID=annotation1583280;Name=R-G;Parent=BBa_J07014
BBa_J07026	.	sequence_alteration	1448	1450	.	+	0	ID=annotation1583298;Name=D-E;Parent=BBa_J07014
BBa_J07026	.	sequence_feature	1001	1027	.	+	0	ID=annotation1582324;Name=CDR3 VL;Parent=BBa_J07014
BBa_J07026	.	sequence_feature	1223	1234	.	+	0	ID=annotation1582328;Name=CDR1 VH;Parent=BBa_J07014
BBa_J07026	.	sequence_alteration	1223	1225	.	+	0	ID=annotation1583293;Name=D-H;Parent=BBa_J07014
BBa_J07026	.	ribosome_entry_site	63	77	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J07026	.	ribosome_entry_site	70	74	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J07026	.	engineered_region	63	77	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J07026	.	ribosome_entry_site	63	77	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J07026	.	terminator	1492	1620	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J07026	.	terminator	1492	1571	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J07026	.	stem_loop	1503	1546	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J07026	.	engineered_region	1492	1571	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J07026	.	terminator	1580	1620	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J07026	.	stop_codon	1613	1613	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J07026	.	stem_loop	1587	1606	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J07026	.	polyA_site	1607	1620	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J07026	.	engineered_region	1580	1620	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J07026	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J07026	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J07026	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J07026	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J07026	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J07026	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J07026	.	CDS	84	713	.	+	0	ID=BBa_J07009;Name=BBa_J07009
BBa_J07026	.	CDS	84	713	.	+	0	ID=annotation1668507;Name=Coding Sequence for ToxR';Parent=BBa_J07009
BBa_J07026	.	sequence_feature	630	677	.	+	0	ID=annotation1668509;Name=Transmembrane Region;Parent=BBa_J07009
BBa_J07026	.	sequence_feature	678	713	.	+	0	ID=annotation1668510;Name=Periplasmic Region;Parent=BBa_J07009
BBa_J07026	.	sequence_feature	84	629	.	+	0	ID=annotation1668506;Name=Cytoplasmic Region;Parent=BBa_J07009
BBa_J07026	.	start_codon	89	91	.	+	0	ID=annotation1668508;Name=Start Codon;Parent=BBa_J07009
>BBa_J07026
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaata
ctagatgttcggattaggacacaactcaaaagagatatcgatgagtcatattggtactaaattcattcttgctgaaaaa
tttaccttcgatcccctaagcaatactctgattgacaaagaagatagtgaagagatcattcgattaggcagcaacgaaa
gccgaatcctttggctgctggcccaacgtccaaacgaggtaatttctcgcaatgatttgcatgactttgtttggcgaga
gcaaggttttgaagtcgatgattccagcttaacccaagccatttcgactctgcgcaaaatgctcaaagattcgacaaag
tccccacaatacgtcaaaacggttccgaagcgcggttaccaattgatcgcccgagtggaaacggttgaagaagagatgg
ctcgcgaaaacgaagctgctcatgacatctctcagccagaatctgtcaatgaatacgcagaatcaagcagtgtgccttc
atcagccactgtagtgaacacaccgcagccagccaatgtcgtggcgaataaatcggctccaaacttggggaatcgactg
tttattctgatagcggtcttacttcccctcgcagtattactgctcactaacccaagccaatccagctttaaacccctaa
cgtactagaggacgtcgttatgactcaaacaccactatcacttcctgttagtctaggtgatcaagcctccatctcttgc
agatctagtcagagccttgtacacagtaatggaaacacctatttacgttggtacctgcaaaagccaggccagtctccaa
aggtcctgatctacaaagtttccaaccgagtttctggggtcccagacaggttcagtggcagtggatcagggacagattt
cacactcaagatcaacagagtggaggctgaggatctgggagtttatttctgctctcaaagtacacatgttccgtggacg
ttcggtggaggcaccaagcttgaaattaagtcctctgctgatgatgctaagaaggatgctgctaagaaggatgatgcta
agaaagatgatgctaagaaagatggtggcgtcaaactggatgagactggaggaggcttggtgcaacctgggggggccat
gaaactctcctgtgttacctctggattcacttttggtcactactggatgaactgggtccgccagtctccagagaaagga
ctggagtgggtagcacaatttagaaacaaaccttataattatgaaacatattattcagattctgtgaaaggcagattca
ccatctcaagagatgattccaaaagtagtgtctacctgcaaatgaacaacttaagagttgaagacaccggtatctatta
ctgtacgggtgcgtcttatggtatggaatacttaggtcaaggaacctcagtcaccgtctcctactagagccaggcatca
aataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagt
cacactggctcaccttcgggtgggcctttctgcgtttata