##gff-version 3
##sequence-region BBa_J07024 1 1620
BBa_J07024	.	terminator	1492	1620	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J07024	.	terminator	1580	1620	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J07024	.	stem_loop	1587	1606	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J07024	.	engineered_region	1580	1620	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J07024	.	stop_codon	1613	1613	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J07024	.	polyA_site	1607	1620	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J07024	.	terminator	1492	1571	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J07024	.	engineered_region	1492	1571	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J07024	.	stem_loop	1503	1546	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J07024	.	CDS	84	713	.	+	0	ID=BBa_J07009;Name=BBa_J07009
BBa_J07024	.	sequence_feature	84	629	.	+	0	ID=annotation1668506;Name=Cytoplasmic Region;Parent=BBa_J07009
BBa_J07024	.	start_codon	89	91	.	+	0	ID=annotation1668508;Name=Start Codon;Parent=BBa_J07009
BBa_J07024	.	sequence_feature	678	713	.	+	0	ID=annotation1668510;Name=Periplasmic Region;Parent=BBa_J07009
BBa_J07024	.	CDS	84	713	.	+	0	ID=annotation1668507;Name=Coding Sequence for ToxR';Parent=BBa_J07009
BBa_J07024	.	sequence_feature	630	677	.	+	0	ID=annotation1668509;Name=Transmembrane Region;Parent=BBa_J07009
BBa_J07024	.	engineered_region	722	1483	.	+	0	ID=BBa_J07012;Name=BBa_J07012
BBa_J07024	.	sequence_feature	1133	1483	.	+	0	ID=annotation1582131;Name=VH;Parent=BBa_J07012
BBa_J07024	.	sequence_feature	722	1057	.	+	0	ID=annotation1582130;Name=VL;Parent=BBa_J07012
BBa_J07024	.	sequence_feature	1280	1336	.	+	0	ID=annotation1582062;Name=CDR2 VH;Parent=BBa_J07012
BBa_J07024	.	sequence_feature	1432	1453	.	+	0	ID=annotation1582123;Name=CDR3 VH;Parent=BBa_J07012
BBa_J07024	.	sequence_feature	1058	1132	.	+	0	ID=annotation1582003;Name=Linker;Parent=BBa_J07012
BBa_J07024	.	sequence_feature	782	841	.	+	0	ID=annotation1581982;Name=CDR1 VL;Parent=BBa_J07012
BBa_J07024	.	sequence_feature	1001	1027	.	+	0	ID=annotation1581987;Name=CDR3 VL;Parent=BBa_J07012
BBa_J07024	.	sequence_feature	884	904	.	+	0	ID=annotation1581986;Name=CDR2 VL;Parent=BBa_J07012
BBa_J07024	.	sequence_feature	1223	1234	.	+	0	ID=annotation1582016;Name=CDR1 VH;Parent=BBa_J07012
BBa_J07024	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J07024	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J07024	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J07024	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J07024	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J07024	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J07024	.	ribosome_entry_site	63	77	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J07024	.	engineered_region	63	77	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J07024	.	ribosome_entry_site	70	74	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J07024	.	ribosome_entry_site	63	77	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
>BBa_J07024
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaata
ctagatgttcggattaggacacaactcaaaagagatatcgatgagtcatattggtactaaattcattcttgctgaaaaa
tttaccttcgatcccctaagcaatactctgattgacaaagaagatagtgaagagatcattcgattaggcagcaacgaaa
gccgaatcctttggctgctggcccaacgtccaaacgaggtaatttctcgcaatgatttgcatgactttgtttggcgaga
gcaaggttttgaagtcgatgattccagcttaacccaagccatttcgactctgcgcaaaatgctcaaagattcgacaaag
tccccacaatacgtcaaaacggttccgaagcgcggttaccaattgatcgcccgagtggaaacggttgaagaagagatgg
ctcgcgaaaacgaagctgctcatgacatctctcagccagaatctgtcaatgaatacgcagaatcaagcagtgtgccttc
atcagccactgtagtgaacacaccgcagccagccaatgtcgtggcgaataaatcggctccaaacttggggaatcgactg
tttattctgatagcggtcttacttcccctcgcagtattactgctcactaacccaagccaatccagctttaaacccctaa
cgtactagaggacgtcgttatgactcaaacaccactatcacttcctgttagtctaggtgatcaagcctccatctcttgc
agatctagtcagagccttgtacacagtaatggaaacacctatttacgttggtacctgcaaaagccaggccagtctccaa
aggtcctgatctacaaagtttccaaccgattttctggggtcccagacaggttcagtggcagtggatcagggacagattt
cacactcaagatcagcagagtggaggctgaggatctgggagtttatttctgctctcaaagtacacatgttccgtggacg
ttcggtggaggcaccaagcttgaaattaagtcctctgctgatgatgctaagaaggatgctgctaagaaggatgatgcta
agaaagatgatgctaagaaagatggtgacgtcaaactggatgagactggaggaggcttggtgcaacctgggaggcccat
gaaactctcctgtgttgcctctggattcacttttagtgactactggatgaactgggtccgccagtctccagagaaagga
ctggagtgggtagcacaaattagaaacaaaccttataattatgaaacatattattcagattctgtgaaaggcagattca
ccatctcaagagatgattccaaaagtagtgtctacctgcaaatgaacaacttaagagttgaagacatgggtatctatta
ctgtacgggttcttactatggtatggactactggggtcaaggaacctcagtcaccgtctcctactagagccaggcatca
aataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagt
cacactggctcaccttcgggtgggcctttctgcgtttata