##gff-version 3
##sequence-region BBa_J06961 1 3309
BBa_J06961	.	ribosome_entry_site	1437	1448	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J06961	.	conserved_region	1441	1444	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J06961	.	CDS	2450	3172	.	+	0	ID=BBa_E0030;Name=BBa_E0030
BBa_J06961	.	terminator	1325	1365	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J06961	.	engineered_region	1325	1365	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J06961	.	stop_codon	1358	1358	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J06961	.	polyA_site	1352	1365	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J06961	.	stem_loop	1332	1351	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J06961	.	promoter	2375	2423	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_J06961	.	promoter	2389	2394	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_J06961	.	engineered_region	2375	2423	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_J06961	.	promoter	2412	2417	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_J06961	.	non_covalent_binding_site	2375	2391	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_J06961	.	non_covalent_binding_site	2399	2415	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_J06961	.	ribosome_entry_site	2432	2443	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J06961	.	conserved_region	2436	2439	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J06961	.	promoter	1374	1427	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_J06961	.	operator	1399	1415	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J06961	.	promoter	1416	1421	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J06961	.	operator	1376	1392	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J06961	.	engineered_region	1374	1427	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J06961	.	promoter	1393	1398	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J06961	.	terminator	3269	3309	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J06961	.	engineered_region	3269	3309	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J06961	.	stop_codon	3302	3302	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J06961	.	polyA_site	3296	3309	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J06961	.	stem_loop	3276	3295	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J06961	.	ribosome_entry_site	58	69	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J06961	.	conserved_region	62	65	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J06961	.	terminator	3181	3260	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J06961	.	stem_loop	3192	3235	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J06961	.	engineered_region	3181	3260	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J06961	.	CDS	76	1228	.	+	0	ID=BBa_J06500;Name=BBa_J06500
BBa_J06961	.	CDS	76	1203	.	+	0	ID=annotation1518213;Name=LacI(ts)-LVA;Parent=BBa_J06500
BBa_J06961	.	engineered_tag	1204	1228	.	+	0	ID=annotation1518229;Name=barcode from LacI (BBa_C0012);Parent=BBa_J06500
BBa_J06961	.	sequence_alteration	805	805	.	+	0	ID=annotation1518230;Name=TS mutation (G->A);Parent=BBa_J06500
BBa_J06961	.	tag	1165	1203	.	+	0	ID=annotation1518220;Name=LVA;Parent=BBa_J06500
BBa_J06961	.	terminator	2326	2366	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J06961	.	engineered_region	2326	2366	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J06961	.	stop_codon	2359	2359	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J06961	.	polyA_site	2353	2366	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J06961	.	stem_loop	2333	2352	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J06961	.	terminator	2238	2317	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J06961	.	stem_loop	2249	2292	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J06961	.	engineered_region	2238	2317	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J06961	.	promoter	1	49	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_J06961	.	promoter	15	20	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_J06961	.	engineered_region	1	49	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_J06961	.	promoter	38	43	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_J06961	.	non_covalent_binding_site	1	17	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_J06961	.	non_covalent_binding_site	25	41	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_J06961	.	CDS	1455	2204	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_J06961	.	tag	2166	2198	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_J06961	.	engineered_tag	2205	2229	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_J06961	.	CDS	1458	2165	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_J06961	.	terminator	1237	1316	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J06961	.	stem_loop	1248	1291	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J06961	.	engineered_region	1237	1316	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_J06961
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatgg
tgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaa
ccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgc
gtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgc
aaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgt
cgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgac
caggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatca
acagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgc
gctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaa
attcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagg
gcatcgttcccactacgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggct
gcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccacc
atcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagg
gcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgc
gttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactac
gctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctca
gtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcg
ggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactg
agcacatactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgc
acgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatgggg
atggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaa
ttctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgca
gccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacc
tttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaag
gtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggc
tgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggt
caggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagtta
tcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgata
gtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttta
tctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatact
agagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactag
atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccaca
agttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaa
gctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatg
aagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggca
actacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaa
ggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcag
aagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagc
agaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaaga
ccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctg
tacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgt
tgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata