##gff-version 3
##sequence-region BBa_J06912 1 2314
BBa_J06912	.	terminator	2186	2265	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J06912	.	stem_loop	2197	2240	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J06912	.	engineered_region	2186	2265	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J06912	.	ribosome_entry_site	1437	1448	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J06912	.	conserved_region	1441	1444	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J06912	.	promoter	1	49	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_J06912	.	engineered_region	1	49	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_J06912	.	promoter	15	20	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_J06912	.	promoter	38	43	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_J06912	.	non_covalent_binding_site	1	17	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_J06912	.	non_covalent_binding_site	25	41	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_J06912	.	terminator	1325	1365	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J06912	.	stem_loop	1332	1351	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J06912	.	stop_codon	1358	1358	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J06912	.	polyA_site	1352	1365	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J06912	.	engineered_region	1325	1365	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J06912	.	promoter	1374	1427	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_J06912	.	promoter	1393	1398	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J06912	.	promoter	1416	1421	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J06912	.	operator	1399	1415	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J06912	.	engineered_region	1374	1427	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J06912	.	operator	1376	1392	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J06912	.	ribosome_entry_site	58	69	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J06912	.	conserved_region	62	65	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J06912	.	terminator	2274	2314	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J06912	.	stem_loop	2281	2300	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J06912	.	stop_codon	2307	2307	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J06912	.	polyA_site	2301	2314	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J06912	.	engineered_region	2274	2314	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J06912	.	CDS	1455	2177	.	+	0	ID=BBa_E0030;Name=BBa_E0030
BBa_J06912	.	terminator	1237	1316	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J06912	.	stem_loop	1248	1291	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J06912	.	engineered_region	1237	1316	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J06912	.	CDS	76	1228	.	+	0	ID=BBa_J06501;Name=BBa_J06501
BBa_J06912	.	tag	1165	1203	.	+	0	ID=annotation1530907;Name=LVA;Parent=BBa_J06501
BBa_J06912	.	CDS	76	1203	.	+	0	ID=annotation1530899;Name=LacI(ts)-LVA;Parent=BBa_J06501
BBa_J06912	.	sequence_alteration	878	878	.	+	0	ID=annotation1530900;Name=TS mutation (G->A);Parent=BBa_J06501
BBa_J06912	.	engineered_tag	1204	1228	.	+	0	ID=annotation1530914;Name=barcode from LacI (BBa_C0012);Parent=BBa_J06501
>BBa_J06912
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatgg
tgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaa
ccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgc
gtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgc
aaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgt
cgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgac
caggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatca
acagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgc
gctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaa
attcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagg
gcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggct
gcgcgttgatgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccacc
atcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagg
gcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgc
gttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactac
gctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctca
gtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcg
ggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactg
agcacatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcct
ggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaag
ctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcc
tgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtcca
ggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtg
aaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaaca
gccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgagga
cggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccac
tacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccg
ccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctc
agtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttc
gggtgggcctttctgcgtttata