##gff-version 3 ##sequence-region BBa_J06025 1 1023 BBa_J06025 . terminator 895 974 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_J06025 . stem_loop 906 949 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_J06025 . engineered_region 895 974 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_J06025 . engineered_region 125 886 . + 0 ID=BBa_E0032;Name=BBa_E0032 BBa_J06025 . stop_codon 881 886 . + 0 ID=annotation2159;Name=2;Parent=BBa_E0032 BBa_J06025 . sequence_alteration 366 366 . + 0 ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032 BBa_J06025 . engineered_region 125 886 . + 0 ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032 BBa_J06025 . CDS 125 886 . + 0 ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032 BBa_J06025 . tag 842 880 . + 0 ID=annotation2160;Name=SsrA;Parent=BBa_E0032 BBa_J06025 . terminator 983 1023 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_J06025 . polyA_site 1010 1023 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_J06025 . engineered_region 983 1023 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_J06025 . stop_codon 1016 1016 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_J06025 . stem_loop 990 1009 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_J06025 . promoter 1 97 . + 0 ID=BBa_R0065;Name=BBa_R0065 BBa_J06025 . non_covalent_binding_site 57 73 . + 0 ID=annotation1986777;Name=OR2 cI;Parent=BBa_R0065 BBa_J06025 . promoter 71 76 . + 0 ID=annotation1986779;Name=-35;Parent=BBa_R0065 BBa_J06025 . promoter 58 58 . + 0 ID=annotation1986778;Name=lux p(R) start;Parent=BBa_R0065 BBa_J06025 . non_covalent_binding_site 6 25 . + 0 ID=annotation1986775;Name=Lux Box;Parent=BBa_R0065 BBa_J06025 . promoter 94 97 . + 0 ID=annotation1986781;Name=-10;Parent=BBa_R0065 BBa_J06025 . non_covalent_binding_site 81 97 . + 0 ID=annotation1986780;Name=OR1 cI;Parent=BBa_R0065 BBa_J06025 . engineered_region 1 97 . + 0 ID=annotation1986774;Name=BBa_R0065;Parent=BBa_R0065 BBa_J06025 . promoter 47 52 . + 0 ID=annotation1986776;Name=-10;Parent=BBa_R0065 BBa_J06025 . ribosome_entry_site 106 118 . + 0 ID=BBa_B0032;Name=BBa_B0032 BBa_J06025 . engineered_region 106 118 . + 0 ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032 BBa_J06025 . ribosome_entry_site 106 118 . + 0 ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032 BBa_J06025 . ribosome_entry_site 112 115 . + 0 ID=annotation1710;Name=RBS;Parent=BBa_B0032 >BBa_J06025 taagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactatt ttacctctggcggtgatatactagagtcacacaggaaagtactagatggtgagcaagggcgaggagctgttcaccgggg tggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgc cacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccacc ttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccg aaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgaggg cgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggag tacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgcc acaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgct gcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctg gagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacg ctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttta tctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata