##gff-version 3
##sequence-region BBa_J05316 1 1419
BBa_J05316	.	ribosome_entry_site	50	61	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J05316	.	conserved_region	54	57	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J05316	.	promoter	1	41	.	+	0	ID=BBa_J05217;Name=BBa_J05217
BBa_J05316	.	engineered_region	542	1419	.	+	0	ID=BBa_E0840;Name=BBa_E0840
BBa_J05316	.	terminator	1379	1419	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_J05316	.	polyA_site	1406	1419	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J05316	.	engineered_region	1379	1419	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J05316	.	stop_codon	1412	1412	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J05316	.	stem_loop	1386	1405	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J05316	.	CDS	563	1282	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_J05316	.	CDS	563	1282	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J05316	.	terminator	1291	1370	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_J05316	.	stem_loop	1302	1345	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J05316	.	engineered_region	1291	1370	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J05316	.	ribosome_entry_site	542	556	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_J05316	.	engineered_region	542	556	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J05316	.	ribosome_entry_site	542	556	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J05316	.	ribosome_entry_site	549	553	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J05316	.	CDS	68	533	.	+	0	ID=BBa_J05110;Name=BBa_J05110
BBa_J05316	.	non_covalent_binding_site	206	229	.	+	0	ID=annotation1730101;Name=ZF-GGC;Parent=BBa_J05110
BBa_J05316	.	CDS	257	340	.	+	0	ID=annotation1730102;Name=Finger 3;Parent=BBa_J05110
BBa_J05316	.	CDS	173	256	.	+	0	ID=annotation1730100;Name=Finger 2;Parent=BBa_J05110
BBa_J05316	.	non_covalent_binding_site	290	313	.	+	0	ID=annotation1730103;Name=ZF-GGA;Parent=BBa_J05110
BBa_J05316	.	non_covalent_binding_site	122	145	.	+	0	ID=annotation1730099;Name=ZF-GGG;Parent=BBa_J05110
BBa_J05316	.	CDS	89	172	.	+	0	ID=annotation1730098;Name=Finger 1;Parent=BBa_J05110
BBa_J05316	.	engineered_tag	509	533	.	+	0	ID=annotation2214043;Name=Help:Barcodes;Parent=BBa_J05110
>BBa_J05316
cctcgcccccgggggcgagggctccggccccggggccggagtactagagaaagaggagaaatactagatgctggaaccg
ggcgaaaaaccgtataaatgcccggaatgcggcaaaagctttagccgcagcgataaactggtgcgccatcagcgcaccc
ataccggcgaaaaaccgtataaatgcccggaatgcggcaaaagctttagcgatccgggccatctggtgcgccatcagcg
cacccataccggcgaaaaaccgtataaatgcccggaatgcggcaaaagctttagccagcgcgcgcatctggaacgccat
cagcgcacccataccggcaaaaaaaccagcacccgcattcaggtgctggaagaaaaagtggaaatgctgcataacctgg
tggatgattggcagcgcaaatataaactgctggaaagcgaatttagcgataccaaagaaaacctgcaaaaaagcattgc
gctgaacaacgaactgcaaaaagcgctgtaataactctgatagtgctagtgtagatctctactagagattaaagaggag
aaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatg
ggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactac
tggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagat
catatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatg
acgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattga
ttttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagac
aaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccatt
atcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttc
gaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggat
gaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttt
atctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata