##gff-version 3
##sequence-region BBa_J04481 1 1105
BBa_J04481	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_J04481	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J04481	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J04481	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J04481	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J04481	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J04481	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J04481	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J04481	.	engineered_region	207	968	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_J04481	.	CDS	207	968	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_J04481	.	tag	924	962	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_J04481	.	engineered_region	207	968	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_J04481	.	stop_codon	963	968	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_J04481	.	sequence_alteration	448	448	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_J04481	.	terminator	977	1056	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J04481	.	stem_loop	988	1031	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J04481	.	engineered_region	977	1056	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J04481	.	terminator	1065	1105	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J04481	.	stop_codon	1098	1098	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J04481	.	stem_loop	1072	1091	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J04481	.	polyA_site	1092	1105	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J04481	.	engineered_region	1065	1105	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_J04481
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagatggtgagcaagggcgaggagctgttcaccg
gggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcga
tgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgacc
accttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgc
ccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcga
gggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctg
gagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatcc
gccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgct
gctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctg
ctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaact
acgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt
ttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata