##gff-version 3 ##sequence-region BBa_J04421 1 1125 BBa_J04421 . terminator 1085 1125 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_J04421 . polyA_site 1112 1125 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_J04421 . stem_loop 1092 1111 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_J04421 . engineered_region 1085 1125 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_J04421 . stop_codon 1118 1118 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_J04421 . promoter 1 200 . + 0 ID=BBa_R0010;Name=BBa_R0010 BBa_J04421 . start_codon 173 173 . + 0 ID=annotation1961227;Name=start;Parent=BBa_R0010 BBa_J04421 . non_covalent_binding_site 89 126 . + 0 ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010 BBa_J04421 . engineered_region 1 200 . + 0 ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010 BBa_J04421 . promoter 161 166 . + 0 ID=annotation1961225;Name=-10;Parent=BBa_R0010 BBa_J04421 . non_covalent_binding_site 166 200 . + 0 ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010 BBa_J04421 . promoter 137 142 . + 0 ID=annotation1961224;Name=-35;Parent=BBa_R0010 BBa_J04421 . CDS 1 88 . + 0 ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010 BBa_J04421 . terminator 997 1076 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_J04421 . stem_loop 1008 1051 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_J04421 . engineered_region 997 1076 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_J04421 . ribosome_entry_site 209 220 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_J04421 . conserved_region 213 216 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_J04421 . engineered_region 227 988 . + 0 ID=BBa_E0022;Name=BBa_E0022 BBa_J04421 . tag 945 982 . + 0 ID=annotation2153;Name=SsrA;Parent=BBa_E0022 BBa_J04421 . CDS 227 988 . + 0 ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022 BBa_J04421 . stop_codon 983 988 . + 0 ID=annotation2154;Name=2;Parent=BBa_E0022 BBa_J04421 . engineered_region 227 988 . + 0 ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022 BBa_J04421 . point_mutation 295 295 . + 0 ID=annotation2155;Name=A;Parent=BBa_E0022 >BBa_J04421 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggtgagcaa gggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtg tccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgc cctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacga cttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacc cgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggca acatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcat caaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacaccccc atcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgaga agcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcc tgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtc gaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggt gggcctttctgcgtttata