##gff-version 3
##sequence-region BBa_J01105 1 1712
BBa_J01105	.	engineered_region	835	1712	.	+	0	ID=BBa_E0840;Name=BBa_E0840
BBa_J01105	.	terminator	1672	1712	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_J01105	.	polyA_site	1699	1712	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J01105	.	engineered_region	1672	1712	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J01105	.	stem_loop	1679	1698	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J01105	.	stop_codon	1705	1705	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J01105	.	ribosome_entry_site	835	849	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_J01105	.	ribosome_entry_site	835	849	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J01105	.	ribosome_entry_site	842	846	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J01105	.	engineered_region	835	849	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J01105	.	CDS	856	1575	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_J01105	.	CDS	856	1575	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J01105	.	terminator	1584	1663	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_J01105	.	stem_loop	1595	1638	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J01105	.	engineered_region	1584	1663	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J01105	.	engineered_region	1	826	.	+	0	ID=BBa_J01104;Name=BBa_J01104
BBa_J01105	.	oriT	1	365	.	+	0	ID=BBa_J01002;Name=BBa_J01002;Parent=BBa_J01104
BBa_J01105	.	engineered_region	374	826	.	+	0	ID=BBa_J01103;Name=BBa_J01103;Parent=BBa_J01104
BBa_J01105	.	promoter	374	422	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_J01103
BBa_J01105	.	non_covalent_binding_site	398	414	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_J01105	.	engineered_region	374	422	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_J01105	.	non_covalent_binding_site	374	390	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_J01105	.	promoter	388	393	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_J01105	.	promoter	411	416	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_J01105	.	engineered_region	431	826	.	+	0	ID=BBa_J01021;Name=BBa_J01021;Parent=BBa_J01103
BBa_J01105	.	ribosome_entry_site	431	442	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J01021
BBa_J01105	.	conserved_region	435	438	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J01105	.	CDS	449	826	.	+	0	ID=BBa_J01001;Name=BBa_J01001;Parent=BBa_J01021
BBa_J01105	.	CDS	449	826	.	+	0	ID=annotation1891611;Name=TraJr;Parent=BBa_J01001
>BBa_J01105
aaggctcaacaggttggtggttctcaccaccaaaagcaccacaccccacgcaaaaacaagtttttgctgatttttcttt
ataaatagagtgttatgaaaaattagtttctcttactctctttatgatatttaaaaaagcggtgtcggcgcggctacaa
caacgcgccgacaccgttttgtaggggtggtactgactatttttataaaaaacattattttatattaggggtgctgcta
gcggcgcggtgtgtttttttataggataccgctaggggcgctgctagcggtgcgtccctgtttgcattatgaattttag
tgtttcgaaattaactttattttatgttcaaaaaaggtaatctctaatgtactagagtaacaccgtgcgtgttgactat
tttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatggctgatgaaaccaagccaaccag
gaagggcagcccacctatcaaggtgtactgccttccagacgaacgaagagcgattgaggaaaaggcggcggcggccggc
atgagcctgtcggcctacctgctggccgtcggccagggctacaaaatcacgggcgtcgtggactatgagcacgtccgcg
agctggcccgcatcaatggcgacctgggccgcctgggcggcctgctgaaactctggctcaccgacgacccgcgcacggc
gcggttcggtgatgccacgatcctcgccctgctggcgaagatcgaagagaagcaggacgagcttggcaaggtcatgatg
ggcgtggtccgcccgagggcagagccatgataataatactagagattaaagaggagaaatactagatgcgtaaaggaga
agaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtgga
gagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggc
caacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgacttttt
caagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgct
gaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattc
ttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagt
taacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggc
gatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagag
accacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatacta
gagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgc
tctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata