##gff-version 3
##sequence-region BBa_J01031 1 1726
BBa_J01031	.	CDS	867	1589	.	+	0	ID=BBa_E0030;Name=BBa_E0030
BBa_J01031	.	engineered_region	109	1726	.	+	0	ID=BBa_J01029;Name=BBa_J01029
BBa_J01031	.	engineered_region	109	626	.	+	0	ID=BBa_J01028;Name=BBa_J01028;Parent=BBa_J01029
BBa_J01031	.	terminator	498	577	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J01028
BBa_J01031	.	engineered_region	498	577	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J01031	.	stem_loop	509	552	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J01031	.	CDS	109	489	.	+	0	ID=BBa_J01004;Name=BBa_J01004;Parent=BBa_J01028
BBa_J01031	.	terminator	586	626	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J01028
BBa_J01031	.	stem_loop	593	612	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J01031	.	engineered_region	586	626	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J01031	.	stop_codon	619	619	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J01031	.	polyA_site	613	626	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J01031	.	engineered_region	635	1726	.	+	0	ID=BBa_J01027;Name=BBa_J01027;Parent=BBa_J01029
BBa_J01031	.	terminator	1598	1677	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J01027
BBa_J01031	.	engineered_region	1598	1677	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J01031	.	stem_loop	1609	1652	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J01031	.	promoter	635	840	.	+	0	ID=BBa_J01005;Name=BBa_J01005;Parent=BBa_J01027
BBa_J01031	.	ribosome_entry_site	849	860	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J01027
BBa_J01031	.	conserved_region	853	856	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J01031	.	terminator	1686	1726	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J01027
BBa_J01031	.	stem_loop	1693	1712	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J01031	.	engineered_region	1686	1726	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J01031	.	stop_codon	1719	1719	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J01031	.	polyA_site	1713	1726	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J01031	.	CDS	867	1589	.	+	0	ID=BBa_E0030;Name=BBa_E0030;Parent=BBa_J01027
BBa_J01031	.	engineered_region	1	102	.	+	0	ID=BBa_J01030;Name=BBa_J01030
BBa_J01031	.	mature_transcript_region	63	102	.	+	0	ID=BBa_J01011;Name=BBa_J01011;Parent=BBa_J01030
BBa_J01031	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J01030
BBa_J01031	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J01031	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J01031	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J01031	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J01031	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
>BBa_J01031
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaactagaatcacctcta
gtgattgggttcaatagaggagatactagatgagcagccagcctgaaccaaagaagaaaaatctcgacgcgagcatcac
aagcattatccatgaaatcggcgtcccagcccatattaaaggctatctctatctgcgcgaagcaatctcaatggtatac
aatgacatcgaattgctcggcagcattacaaaagtcctctatccggacatcgccaaaaaatttaacacaaccgcaagcc
gtgtagaaagagcgatccgccatgcaattgaagtggcatggagcagaggaaacattgattccatttcctcgttgtttgg
ttatactgtcagcatgacaaaagctaaacctaccaacagtgaatttattgcaatggttgcggataagctgaggttagag
cataaggcttcttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatc
tgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactag
agcgggtcatcctaacaaatcggtttctcttgcagaagccgatttttttcatttttagacaacattccggaaattcttt
tcataaacgaatatcaaggcagaaaccgtcgaagatttctttggtattgttaccttcttttgacaaaatcctatctgtg
ctttcgctataatgacaggcaacgaatataacaggtgggagatgagaggatactagagaaagaggagaaatactagatg
gtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagt
tcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagct
gcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaag
ctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaact
acaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaagga
ggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaag
aacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcaga
acacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccc
caacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtac
aagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata