##gff-version 3 ##sequence-region BBa_I9124 1 6176 BBa_I9124 . terminator 3081 3121 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_I9124 . polyA_site 3108 3121 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_I9124 . stem_loop 3088 3107 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_I9124 . engineered_region 3081 3121 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_I9124 . stop_codon 3114 3114 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_I9124 . terminator 938 978 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_I9124 . polyA_site 965 978 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_I9124 . stem_loop 945 964 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_I9124 . engineered_region 938 978 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_I9124 . stop_codon 971 971 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_I9124 . promoter 987 2196 . + 0 ID=BBa_I0500;Name=BBa_I0500 BBa_I9124 . promoter 2029 2100 . + 0 ID=annotation1498842;Name=PC;Parent=BBa_I0500 BBa_I9124 . promoter 2066 2196 . + 0 ID=annotation1498841;Name=Pbad;Parent=BBa_I0500 BBa_I9124 . sequence_feature 987 2065 . + 0 ID=annotation1498843;Name=AraC;Parent=BBa_I0500 BBa_I9124 . ribosome_entry_site 2205 2216 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_I9124 . conserved_region 2209 2212 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_I9124 . CDS 4366 5007 . + 0 ID=BBa_C0070;Name=BBa_C0070 BBa_I9124 . CDS 4366 4968 . + 0 ID=annotation301203;Name=rhlL;Parent=BBa_C0070 BBa_I9124 . engineered_tag 5008 5032 . + 0 ID=annotation2213996;Name=Help:Barcodes;Parent=BBa_C0070 BBa_I9124 . tag 4969 5001 . + 0 ID=annotation306820;Name=LVA;Parent=BBa_C0070 BBa_I9124 . terminator 5129 5169 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_I9124 . polyA_site 5156 5169 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_I9124 . stem_loop 5136 5155 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_I9124 . engineered_region 5129 5169 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_I9124 . stop_codon 5162 5162 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_I9124 . engineered_region 2223 2984 . + 0 ID=BBa_E0022;Name=BBa_E0022 BBa_I9124 . tag 2941 2978 . + 0 ID=annotation2153;Name=SsrA;Parent=BBa_E0022 BBa_I9124 . CDS 2223 2984 . + 0 ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022 BBa_I9124 . stop_codon 2979 2984 . + 0 ID=annotation2154;Name=2;Parent=BBa_E0022 BBa_I9124 . engineered_region 2223 2984 . + 0 ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022 BBa_I9124 . point_mutation 2291 2291 . + 0 ID=annotation2155;Name=A;Parent=BBa_E0022 BBa_I9124 . engineered_region 80 841 . + 0 ID=BBa_E0032;Name=BBa_E0032 BBa_I9124 . stop_codon 836 841 . + 0 ID=annotation2159;Name=2;Parent=BBa_E0032 BBa_I9124 . sequence_alteration 321 321 . + 0 ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032 BBa_I9124 . engineered_region 80 841 . + 0 ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032 BBa_I9124 . CDS 80 841 . + 0 ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032 BBa_I9124 . tag 797 835 . + 0 ID=annotation2160;Name=SsrA;Parent=BBa_E0032 BBa_I9124 . ribosome_entry_site 62 73 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_I9124 . conserved_region 66 69 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_I9124 . terminator 2993 3072 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_I9124 . stem_loop 3004 3047 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_I9124 . engineered_region 2993 3072 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_I9124 . promoter 3130 4339 . + 0 ID=BBa_I0500;Name=BBa_I0500 BBa_I9124 . promoter 4172 4243 . + 0 ID=annotation1498842;Name=PC;Parent=BBa_I0500 BBa_I9124 . promoter 4209 4339 . + 0 ID=annotation1498841;Name=Pbad;Parent=BBa_I0500 BBa_I9124 . sequence_feature 3130 4208 . + 0 ID=annotation1498843;Name=AraC;Parent=BBa_I0500 BBa_I9124 . ribosome_entry_site 5235 5246 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_I9124 . conserved_region 5239 5242 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_I9124 . terminator 6048 6127 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_I9124 . stem_loop 6059 6102 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_I9124 . engineered_region 6048 6127 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_I9124 . terminator 5041 5120 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_I9124 . stem_loop 5052 5095 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_I9124 . engineered_region 5041 5120 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_I9124 . promoter 1 53 . + 0 ID=BBa_R0071;Name=BBa_R0071 BBa_I9124 . promoter 19 24 . + 0 ID=annotation297103;Name=-35;Parent=BBa_R0071 BBa_I9124 . promoter 42 47 . + 0 ID=annotation297104;Name=-10;Parent=BBa_R0071 BBa_I9124 . non_covalent_binding_site 1 20 . + 0 ID=annotation297102;Name=RhlR;Parent=BBa_R0071 BBa_I9124 . start_codon 53 53 . + 0 ID=annotation297105;Name=start;Parent=BBa_R0071 BBa_I9124 . terminator 850 929 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_I9124 . stem_loop 861 904 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_I9124 . engineered_region 850 929 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_I9124 . promoter 5178 5226 . + 0 ID=BBa_R1075;Name=BBa_R1075 BBa_I9124 . promoter 5212 5217 . + 0 ID=annotation309020;Name=-10;Parent=BBa_R1075 BBa_I9124 . promoter 5192 5197 . + 0 ID=annotation309024;Name=-35;Parent=BBa_R1075 BBa_I9124 . ribosome_entry_site 4348 4359 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_I9124 . conserved_region 4352 4355 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_I9124 . terminator 6136 6176 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_I9124 . polyA_site 6163 6176 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_I9124 . stem_loop 6143 6162 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_I9124 . engineered_region 6136 6176 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_I9124 . stop_codon 6169 6169 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_I9124 . CDS 5253 6014 . + 0 ID=BBa_C0071;Name=BBa_C0071 BBa_I9124 . CDS 5253 5975 . + 0 ID=annotation301192;Name=rhlR;Parent=BBa_C0071 BBa_I9124 . tag 5976 6008 . + 0 ID=annotation306523;Name=LVA;Parent=BBa_C0071 BBa_I9124 . engineered_tag 6015 6039 . + 0 ID=annotation2213995;Name=Help:Barcodes;Parent=BBa_C0071 >BBa_I9124 tcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttctactagagaaagaggagaaatactag atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccaca agttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaa gctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatg aagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggca actacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaa ggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcag aagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagc agaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaaga ccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctg tacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacga aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct caccttcgggtgggcctttctgcgtttatatactagagttatgacaacttgacggctacatcattcactttttcttcac aaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaacc aacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcg cgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtg cgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccat cggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctcc gaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccg ggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaac ccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatca cccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctt tcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggc attaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaa accaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccc cgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacgg cagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggat cctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagaaagagga gaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtg aacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgca ccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccc cgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaag gacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggca tcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgc cgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgac cactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccc tgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcat ggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatca aataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagt cacactggctcaccttcgggtgggcctttctgcgtttatatactagagttatgacaacttgacggctacatcattcact ttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgat cgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacg ttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaa acatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtaccc gattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgc cagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgc gcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggac gaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacag caaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgaga atataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgcca ccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccatt cagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaa ccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtct ataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataag attagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactag agaaagaggagaaatactagatgatcgaactgctgtccgaatccctggaaggtctgtccgctgctatgatcgctgaact gggtcgttaccgtcaccaggttttcatcgaaaaactgggttgggacgttgtttccacctcccgtgttcgtgaccaggag ttcgaccagttcgaccacccgcagacccgttacatcgttgctatgtcccgtcagggtatctgcggttgcgctcgtctgc tgccgaccaccgacgcttacctgctgaaagacgttttcgcttacctgtgctccgaaaccccgccgtccgacccgtccgt ttgggaactgtcccgttacgctgcttccgctgctgacgacccgcagctggctatgaaaatcttctggtcctccctccag tgcgcttggtacctgggtgcttcctccgttgttgctgttaccaccaccgctatggaacgttacttcgttcgtaacggtg ttatcctccagcgtctgggtccgccgcagaaagttaaaggtgaaaccctggttgctatctccttcccggcttaccagga acgtggtctggaaatgctgctgcgttaccacccggaatggctccagggtgttccgctgtccatggctgttgctgcaaac gacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaa acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact ggctcaccttcgggtgggcctttctgcgtttatatactagagttaaatttcctcttttcaggccggaataactccctat aatgcgccaccatactagagaaagaggagaaatactagatgaggaatgacggaggctttttgctgtggtgggacggttt gcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttc gattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaagg cctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaat ggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggc gcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagct tcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatcc gatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttcc ggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacg cgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatcgctgcaaacgacgaaaactacgctttagt agcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaag actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc tttctgcgtttata