##gff-version 3
##sequence-region BBa_I9120 1 6148
BBa_I9120	.	promoter	5156	5204	.	+	0	ID=BBa_R1075;Name=BBa_R1075
BBa_I9120	.	promoter	5170	5175	.	+	0	ID=annotation309024;Name=-35;Parent=BBa_R1075
BBa_I9120	.	promoter	5190	5195	.	+	0	ID=annotation309020;Name=-10;Parent=BBa_R1075
BBa_I9120	.	ribosome_entry_site	4350	4361	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I9120	.	conserved_region	4354	4357	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I9120	.	terminator	3083	3123	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I9120	.	stem_loop	3090	3109	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I9120	.	polyA_site	3110	3123	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I9120	.	stop_codon	3116	3116	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I9120	.	engineered_region	3083	3123	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I9120	.	CDS	4368	4985	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_I9120	.	engineered_tag	4986	5010	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_I9120	.	tag	4947	4978	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_I9120	.	engineered_region	4368	4985	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_I9120	.	CDS	4368	4946	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_I9120	.	terminator	6020	6099	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I9120	.	stem_loop	6031	6074	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I9120	.	engineered_region	6020	6099	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I9120	.	promoter	3132	4341	.	+	0	ID=BBa_I0500;Name=BBa_I0500
BBa_I9120	.	promoter	4211	4341	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_I9120	.	sequence_feature	3132	4210	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_I9120	.	promoter	4174	4245	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_I9120	.	terminator	6108	6148	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I9120	.	stem_loop	6115	6134	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I9120	.	polyA_site	6135	6148	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I9120	.	stop_codon	6141	6141	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I9120	.	engineered_region	6108	6148	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I9120	.	promoter	989	2198	.	+	0	ID=BBa_I0500;Name=BBa_I0500
BBa_I9120	.	promoter	2068	2198	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_I9120	.	sequence_feature	989	2067	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_I9120	.	promoter	2031	2102	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_I9120	.	terminator	5107	5147	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I9120	.	stem_loop	5114	5133	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I9120	.	polyA_site	5134	5147	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I9120	.	stop_codon	5140	5140	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I9120	.	engineered_region	5107	5147	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I9120	.	ribosome_entry_site	2207	2218	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I9120	.	conserved_region	2211	2214	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I9120	.	promoter	1	55	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_I9120	.	engineered_region	1	55	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_I9120	.	promoter	20	25	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_I9120	.	start_codon	53	53	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_I9120	.	promoter	42	47	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_I9120	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_I9120	.	ribosome_entry_site	5213	5224	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I9120	.	conserved_region	5217	5220	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I9120	.	terminator	2995	3074	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I9120	.	stem_loop	3006	3049	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I9120	.	engineered_region	2995	3074	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I9120	.	terminator	940	980	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I9120	.	stem_loop	947	966	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I9120	.	polyA_site	967	980	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I9120	.	stop_codon	973	973	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I9120	.	engineered_region	940	980	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I9120	.	CDS	5231	5986	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_I9120	.	engineered_tag	5987	6011	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I9120	.	engineered_region	5231	5232	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I9120	.	point_mutation	5404	5404	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I9120	.	engineered_region	5231	5986	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I9120	.	point_mutation	5722	5722	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I9120	.	CDS	5231	5980	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I9120	.	terminator	5019	5098	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I9120	.	stem_loop	5030	5073	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I9120	.	engineered_region	5019	5098	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I9120	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I9120	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I9120	.	terminator	852	931	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I9120	.	stem_loop	863	906	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I9120	.	engineered_region	852	931	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I9120	.	engineered_region	82	843	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_I9120	.	sequence_alteration	323	323	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_I9120	.	engineered_region	82	843	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_I9120	.	stop_codon	838	843	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_I9120	.	tag	799	837	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_I9120	.	CDS	82	843	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_I9120	.	engineered_region	2225	2986	.	+	0	ID=BBa_E0022;Name=BBa_E0022
BBa_I9120	.	tag	2943	2980	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_I9120	.	stop_codon	2981	2986	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_I9120	.	engineered_region	2225	2986	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_I9120	.	point_mutation	2293	2293	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_I9120	.	CDS	2225	2986	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
>BBa_I9120
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatact
agatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggcca
caagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggc
aagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccaca
tgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacgg
caactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttc
aaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagc
agaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactacca
gcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaa
gaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagc
tgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaac
gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttatatactagagttatgacaacttgacggctacatcattcactttttcttc
acaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaa
ccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcct
cgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctg
tgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatcc
atcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagct
ccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatc
cgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaa
acccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatat
cacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacc
tttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgg
gcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaag
aaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaac
cccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcac
ggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcgg
atcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagaaagag
gagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacg
tgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctg
caccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctac
cccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttca
aggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaaggg
catcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcacc
gccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccg
accactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgc
cctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggc
atggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttatgacaacttgacggctacatcattca
ctttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttg
atcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgata
cgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagc
aaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtac
ccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatc
gccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggt
gcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcgg
acgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaac
agcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattga
gaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgc
caccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgcca
ttcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaacca
aaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgt
ctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccata
agattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctact
agagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaa
ggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcag
atgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttatt
acctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaat
atagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaac
tatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcg
atttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggtt
gtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtag
cttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagac
tgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctt
tctgcgtttatatactagagttaaatttcctcttttcaggccggaataactccctataatgcgccaccatactagagaa
agaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagc
aataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatc
ctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaa
tttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaat
gctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattc
atacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttaca
tgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatca
aacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaa
tattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgcca
aagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgt
agatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt
gtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata