##gff-version 3
##sequence-region BBa_I9101 1 4829
BBa_I9101	.	ribosome_entry_site	1219	1230	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I9101	.	conserved_region	1223	1226	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I9101	.	engineered_region	3931	4692	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_I9101	.	stop_codon	4687	4692	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_I9101	.	sequence_alteration	4172	4172	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_I9101	.	CDS	3931	4692	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_I9101	.	engineered_region	3931	4692	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_I9101	.	tag	4648	4686	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_I9101	.	terminator	3701	3780	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I9101	.	stem_loop	3712	3755	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I9101	.	engineered_region	3701	3780	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I9101	.	terminator	2007	2086	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I9101	.	stem_loop	2018	2061	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I9101	.	engineered_region	2007	2086	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I9101	.	terminator	3789	3829	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I9101	.	stop_codon	3822	3822	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I9101	.	polyA_site	3816	3829	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I9101	.	stem_loop	3796	3815	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I9101	.	engineered_region	3789	3829	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I9101	.	terminator	4701	4780	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I9101	.	stem_loop	4712	4755	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I9101	.	engineered_region	4701	4780	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I9101	.	promoter	3838	3904	.	+	0	ID=BBa_R0073;Name=BBa_R0073
BBa_I9101	.	operator	3838	3904	.	+	0	ID=annotation302669;Name=mnt;Parent=BBa_R0073
BBa_I9101	.	promoter	3884	3889	.	+	0	ID=annotation318643;Name=-10;Parent=BBa_R0073
BBa_I9101	.	non_covalent_binding_site	3869	3875	.	+	0	ID=annotation318638;Name=dimer right half;Parent=BBa_R0073
BBa_I9101	.	non_covalent_binding_site	3860	3866	.	+	0	ID=annotation318636;Name=dimer left half;Parent=BBa_R0073
BBa_I9101	.	terminator	2095	2135	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I9101	.	stop_codon	2128	2128	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I9101	.	polyA_site	2122	2135	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I9101	.	stem_loop	2102	2121	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I9101	.	engineered_region	2095	2135	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I9101	.	CDS	3380	3667	.	+	0	ID=BBa_C0073;Name=BBa_C0073
BBa_I9101	.	CDS	3380	3628	.	+	0	ID=annotation302631;Name=mnt;Parent=BBa_C0073
BBa_I9101	.	tag	3629	3661	.	+	0	ID=annotation306535;Name=LVA;Parent=BBa_C0073
BBa_I9101	.	engineered_tag	3668	3692	.	+	0	ID=annotation2214006;Name=Help:Barcodes;Parent=BBa_C0073
BBa_I9101	.	sequence_alteration	3410	3412	.	+	0	ID=annotation308213;Name=Arg->Lys (ctg);Parent=BBa_C0073
BBa_I9101	.	point_mutation	3474	3476	.	+	0	ID=annotation308457;Name=T;Parent=BBa_C0073
BBa_I9101	.	ribosome_entry_site	3362	3373	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I9101	.	conserved_region	3366	3369	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I9101	.	engineered_region	1237	1998	.	+	0	ID=BBa_E0022;Name=BBa_E0022
BBa_I9101	.	tag	1955	1992	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_I9101	.	CDS	1237	1998	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_I9101	.	engineered_region	1237	1998	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_I9101	.	stop_codon	1993	1998	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_I9101	.	point_mutation	1305	1305	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_I9101	.	terminator	4789	4829	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I9101	.	stop_codon	4822	4822	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I9101	.	polyA_site	4816	4829	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I9101	.	stem_loop	4796	4815	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I9101	.	engineered_region	4789	4829	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I9101	.	ribosome_entry_site	3913	3924	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I9101	.	conserved_region	3917	3920	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I9101	.	promoter	1	1210	.	+	0	ID=BBa_I0500;Name=BBa_I0500
BBa_I9101	.	promoter	1080	1210	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_I9101	.	sequence_feature	1	1079	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_I9101	.	promoter	1043	1114	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_I9101	.	promoter	2144	3353	.	+	0	ID=BBa_I0500;Name=BBa_I0500
BBa_I9101	.	promoter	3223	3353	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_I9101	.	sequence_feature	2144	3222	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_I9101	.	promoter	3186	3257	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
>BBa_I9101
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat
tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg
tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa
aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga
tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc
gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg
cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag
ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac
cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac
caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa
ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg
cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg
cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa
gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac
tttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttc
tccatacccgtttttttgggctagctactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttca
ccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgaggg
cgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtg
accaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgcca
tgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagtt
cgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaag
ctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaaga
tccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgt
gctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtc
ctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaa
actacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttc
gttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttta
tatactagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggc
cccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggc
atccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaact
gctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtc
tgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgct
tccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgt
taatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatat
tgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctcc
ggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccct
gatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaa
atcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcagggga
tcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacatt
gccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaag
cgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcac
ggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactct
ctactgtttctccatacccgtttttttgggctagctactagagaaagaggagaaatactagatggcccgggatgatcct
cacttcaattttaaaatgccaatggaagtaagagagaaattgaaatttagagcagaggcaaacggacggagcatgaact
ctgagcttttgcaaatcgtacaagatgccctaagcaaaccgtcaccagtcactgggtaccgcaatgatgcggaacgact
cgccgatgagcagagcgagttagtgaagaagatggtcttcgatacactgaaggatctttataaaaaaaccaccgctgca
aacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaat
aaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcac
actggctcaccttcgggtgggcctttctgcgtttatatactagagctcgaggtgagtgcacagtactaggtccacggtg
acctagatctcctatagtgagtcgtattaattttactagagaaagaggagaaatactagatggtgagcaagggcgagga
gctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgag
ggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggccca
ccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaa
gtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgag
gtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctgg
ggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaa
cttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgac
ggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatc
acatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaa
cgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactg
ggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttc
tgcgtttata