##gff-version 3
##sequence-region BBa_I8033 1 1990
BBa_I8033	.	promoter	1013	1065	.	+	0	ID=BBa_R0071;Name=BBa_R0071
BBa_I8033	.	start_codon	1065	1065	.	+	0	ID=annotation297105;Name=start;Parent=BBa_R0071
BBa_I8033	.	promoter	1031	1036	.	+	0	ID=annotation297103;Name=-35;Parent=BBa_R0071
BBa_I8033	.	promoter	1054	1059	.	+	0	ID=annotation297104;Name=-10;Parent=BBa_R0071
BBa_I8033	.	non_covalent_binding_site	1013	1032	.	+	0	ID=annotation297102;Name=RhlR;Parent=BBa_R0071
BBa_I8033	.	terminator	876	955	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I8033	.	stem_loop	887	930	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I8033	.	engineered_region	876	955	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I8033	.	CDS	81	842	.	+	0	ID=BBa_C0071;Name=BBa_C0071
BBa_I8033	.	CDS	81	803	.	+	0	ID=annotation301192;Name=rhlR;Parent=BBa_C0071
BBa_I8033	.	engineered_tag	843	867	.	+	0	ID=annotation2213995;Name=Help:Barcodes;Parent=BBa_C0071
BBa_I8033	.	tag	804	836	.	+	0	ID=annotation306523;Name=LVA;Parent=BBa_C0071
BBa_I8033	.	terminator	1862	1941	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I8033	.	stem_loop	1873	1916	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I8033	.	engineered_region	1862	1941	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I8033	.	terminator	1950	1990	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I8033	.	stop_codon	1983	1983	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I8033	.	stem_loop	1957	1976	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I8033	.	polyA_site	1977	1990	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I8033	.	engineered_region	1950	1990	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I8033	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I8033	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I8033	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I8033	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I8033	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I8033	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I8033	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I8033	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I8033	.	ribosome_entry_site	1074	1085	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I8033	.	conserved_region	1078	1081	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I8033	.	terminator	964	1004	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I8033	.	stop_codon	997	997	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I8033	.	stem_loop	971	990	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I8033	.	polyA_site	991	1004	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I8033	.	engineered_region	964	1004	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I8033	.	engineered_region	1092	1853	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_I8033	.	CDS	1092	1853	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_I8033	.	tag	1809	1847	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_I8033	.	engineered_region	1092	1853	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_I8033	.	stop_codon	1848	1853	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_I8033	.	sequence_alteration	1333	1333	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
>BBa_I8033
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggc
gtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattccct
tcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggc
cgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccgg
atgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctca
gcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcat
gatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgc
gaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagca
cggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggc
gctgggtctcatcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcacta
ctagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaa
cgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcctgtgaaatctgg
cagttaccgttagctttcgaattggctaaaaagtgttctactagagaaagaggagaaatactagatggtgagcaagggc
gaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccg
gcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctg
gcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttc
ttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcg
ccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacat
cctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaag
gtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcg
gcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcg
cgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgct
gcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaa
gactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggc
ctttctgcgtttata