##gff-version 3
##sequence-region BBa_I8030 1 3541
BBa_I8030	.	terminator	2427	2506	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I8030	.	stem_loop	2438	2481	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I8030	.	engineered_region	2427	2506	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I8030	.	CDS	81	740	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_I8030	.	CDS	84	700	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I8030	.	tag	701	734	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I8030	.	engineered_tag	741	765	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I8030	.	terminator	3501	3541	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I8030	.	polyA_site	3528	3541	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I8030	.	engineered_region	3501	3541	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I8030	.	stop_codon	3534	3534	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I8030	.	stem_loop	3508	3527	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I8030	.	engineered_region	2643	3404	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_I8030	.	stop_codon	3399	3404	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_I8030	.	sequence_alteration	2884	2884	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_I8030	.	engineered_region	2643	3404	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_I8030	.	CDS	2643	3404	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_I8030	.	tag	3360	3398	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_I8030	.	terminator	3413	3492	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I8030	.	stem_loop	3424	3467	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I8030	.	engineered_region	3413	3492	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I8030	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I8030	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I8030	.	CDS	792	1553	.	+	0	ID=BBa_C0071;Name=BBa_C0071
BBa_I8030	.	CDS	792	1514	.	+	0	ID=annotation301192;Name=rhlR;Parent=BBa_C0071
BBa_I8030	.	engineered_tag	1554	1578	.	+	0	ID=annotation2213995;Name=Help:Barcodes;Parent=BBa_C0071
BBa_I8030	.	tag	1515	1547	.	+	0	ID=annotation306523;Name=LVA;Parent=BBa_C0071
BBa_I8030	.	terminator	2515	2555	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I8030	.	polyA_site	2542	2555	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I8030	.	engineered_region	2515	2555	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I8030	.	stop_codon	2548	2548	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I8030	.	stem_loop	2522	2541	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I8030	.	ribosome_entry_site	774	785	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I8030	.	conserved_region	778	781	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I8030	.	ribosome_entry_site	1587	1598	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I8030	.	conserved_region	1591	1594	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I8030	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I8030	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I8030	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I8030	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I8030	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I8030	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I8030	.	CDS	1605	2393	.	+	0	ID=BBa_C0060;Name=BBa_C0060
BBa_I8030	.	tag	2355	2387	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_I8030	.	CDS	1605	2354	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_I8030	.	engineered_tag	2394	2418	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_I8030	.	start_codon	1605	1607	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_I8030	.	stop_codon	2388	2393	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_I8030	.	engineered_region	1605	2393	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_I8030	.	ribosome_entry_site	2625	2636	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I8030	.	conserved_region	2629	2632	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I8030	.	promoter	2564	2616	.	+	0	ID=BBa_R0071;Name=BBa_R0071
BBa_I8030	.	promoter	2582	2587	.	+	0	ID=annotation297103;Name=-35;Parent=BBa_R0071
BBa_I8030	.	promoter	2605	2610	.	+	0	ID=annotation297104;Name=-10;Parent=BBa_R0071
BBa_I8030	.	non_covalent_binding_site	2564	2583	.	+	0	ID=annotation297102;Name=RhlR;Parent=BBa_R0071
BBa_I8030	.	start_codon	2616	2616	.	+	0	ID=annotation297105;Name=start;Parent=BBa_R0071
>BBa_I8030
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaaca
acccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcg
acgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagatttttt
acgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcct
acagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattat
atgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaag
ggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagcca
gccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacg
acgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatacta
gatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggc
gtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattccct
tcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggc
cgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccgg
atgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctca
gcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcat
gatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgc
gaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagca
cggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggc
gctgggtctcatcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcacta
ctagagaaagaggagaaatactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcatt
cgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaagg
acctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaaggg
caggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttc
tttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtaca
gcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatc
attgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctat
taattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagt
gccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccg
attgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaa
actacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcac
cttcgggtgggcctttctgcgtttatatactagagtcctgtgaaatctggcagttaccgttagctttcgaattggctaa
aaagtgttctactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccat
cctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggc
aagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacg
gcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgt
ccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctg
gtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactaca
acagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcga
ggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaac
cactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtga
ccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagc
ttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt
gtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata