##gff-version 3 ##sequence-region BBa_I8003 1 6698 BBa_I8003 . terminator 4040 4119 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_I8003 . stem_loop 4051 4094 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_I8003 . engineered_region 4040 4119 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_I8003 . CDS 1563 2180 . + 0 ID=BBa_C0061;Name=BBa_C0061 BBa_I8003 . engineered_region 1563 2180 . + 0 ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061 BBa_I8003 . engineered_tag 2181 2205 . + 0 ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061 BBa_I8003 . CDS 1563 2141 . + 0 ID=annotation1761;Name=luxI;Parent=BBa_C0061 BBa_I8003 . tag 2142 2173 . + 0 ID=annotation1760;Name=LVA;Parent=BBa_C0061 BBa_I8003 . CDS 4968 5729 . + 0 ID=BBa_C0071;Name=BBa_C0071 BBa_I8003 . engineered_tag 5730 5754 . + 0 ID=annotation2213995;Name=Help:Barcodes;Parent=BBa_C0071 BBa_I8003 . CDS 4968 5690 . + 0 ID=annotation301192;Name=rhlR;Parent=BBa_C0071 BBa_I8003 . tag 5691 5723 . + 0 ID=annotation306523;Name=LVA;Parent=BBa_C0071 BBa_I8003 . engineered_region 82 843 . + 0 ID=BBa_E0022;Name=BBa_E0022 BBa_I8003 . CDS 82 843 . + 0 ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022 BBa_I8003 . tag 800 837 . + 0 ID=annotation2153;Name=SsrA;Parent=BBa_E0022 BBa_I8003 . stop_codon 838 843 . + 0 ID=annotation2154;Name=2;Parent=BBa_E0022 BBa_I8003 . engineered_region 82 843 . + 0 ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022 BBa_I8003 . point_mutation 150 150 . + 0 ID=annotation2155;Name=A;Parent=BBa_E0022 BBa_I8003 . terminator 2302 2342 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_I8003 . polyA_site 2329 2342 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_I8003 . engineered_region 2302 2342 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_I8003 . stop_codon 2335 2335 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_I8003 . stem_loop 2309 2328 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_I8003 . promoter 2351 2403 . + 0 ID=BBa_R0071;Name=BBa_R0071 BBa_I8003 . promoter 2392 2397 . + 0 ID=annotation297104;Name=-10;Parent=BBa_R0071 BBa_I8003 . non_covalent_binding_site 2351 2370 . + 0 ID=annotation297102;Name=RhlR;Parent=BBa_R0071 BBa_I8003 . promoter 2369 2374 . + 0 ID=annotation297103;Name=-35;Parent=BBa_R0071 BBa_I8003 . start_codon 2403 2403 . + 0 ID=annotation297105;Name=start;Parent=BBa_R0071 BBa_I8003 . terminator 4128 4168 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_I8003 . polyA_site 4155 4168 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_I8003 . engineered_region 4128 4168 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_I8003 . stop_codon 4161 4161 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_I8003 . stem_loop 4135 4154 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_I8003 . terminator 6570 6649 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_I8003 . stem_loop 6581 6624 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_I8003 . engineered_region 6570 6649 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_I8003 . ribosome_entry_site 4239 4250 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_I8003 . conserved_region 4243 4246 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_I8003 . ribosome_entry_site 852 863 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_I8003 . conserved_region 856 859 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_I8003 . terminator 2214 2293 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_I8003 . stem_loop 2225 2268 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_I8003 . engineered_region 2214 2293 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_I8003 . CDS 4257 4916 . + 0 ID=BBa_C0040;Name=BBa_C0040 BBa_I8003 . tag 4877 4910 . + 0 ID=annotation23330;Name=SsrA;Parent=BBa_C0040 BBa_I8003 . CDS 4260 4876 . + 0 ID=annotation23329;Name=tetR;Parent=BBa_C0040 BBa_I8003 . engineered_tag 4917 4941 . + 0 ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040 BBa_I8003 . engineered_region 2430 3191 . + 0 ID=BBa_E0032;Name=BBa_E0032 BBa_I8003 . CDS 2430 3191 . + 0 ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032 BBa_I8003 . tag 3147 3185 . + 0 ID=annotation2160;Name=SsrA;Parent=BBa_E0032 BBa_I8003 . sequence_alteration 2671 2671 . + 0 ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032 BBa_I8003 . engineered_region 2430 3191 . + 0 ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032 BBa_I8003 . stop_codon 3186 3191 . + 0 ID=annotation2159;Name=2;Parent=BBa_E0032 BBa_I8003 . promoter 4177 4230 . + 0 ID=BBa_R0040;Name=BBa_R0040 BBa_I8003 . engineered_region 4177 4230 . + 0 ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040 BBa_I8003 . non_covalent_binding_site 4202 4220 . + 0 ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040 BBa_I8003 . promoter 4196 4201 . + 0 ID=annotation1986785;Name=-35;Parent=BBa_R0040 BBa_I8003 . promoter 4219 4224 . + 0 ID=annotation1986787;Name=-10;Parent=BBa_R0040 BBa_I8003 . non_covalent_binding_site 4177 4195 . + 0 ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040 BBa_I8003 . CDS 3218 4006 . + 0 ID=BBa_C0060;Name=BBa_C0060 BBa_I8003 . tag 3968 4000 . + 0 ID=annotation1756;Name=LVA;Parent=BBa_C0060 BBa_I8003 . stop_codon 4001 4006 . + 0 ID=annotation1755;Name=2;Parent=BBa_C0060 BBa_I8003 . start_codon 3218 3220 . + 0 ID=annotation1754;Name=start;Parent=BBa_C0060 BBa_I8003 . engineered_tag 4007 4031 . + 0 ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060 BBa_I8003 . CDS 3218 3967 . + 0 ID=annotation1757;Name=aiiA;Parent=BBa_C0060 BBa_I8003 . engineered_region 3218 4006 . + 0 ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060 BBa_I8003 . ribosome_entry_site 64 75 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_I8003 . conserved_region 68 71 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_I8003 . CDS 870 1511 . + 0 ID=BBa_C0070;Name=BBa_C0070 BBa_I8003 . CDS 870 1472 . + 0 ID=annotation301203;Name=rhlL;Parent=BBa_C0070 BBa_I8003 . tag 1473 1505 . + 0 ID=annotation306820;Name=LVA;Parent=BBa_C0070 BBa_I8003 . engineered_tag 1512 1536 . + 0 ID=annotation2213996;Name=Help:Barcodes;Parent=BBa_C0070 BBa_I8003 . ribosome_entry_site 4950 4961 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_I8003 . conserved_region 4954 4957 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_I8003 . terminator 6658 6698 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_I8003 . polyA_site 6685 6698 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_I8003 . engineered_region 6658 6698 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_I8003 . stop_codon 6691 6691 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_I8003 . stem_loop 6665 6684 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_I8003 . ribosome_entry_site 2412 2423 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_I8003 . conserved_region 2416 2419 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_I8003 . CDS 5781 6536 . + 0 ID=BBa_C0062;Name=BBa_C0062 BBa_I8003 . CDS 5781 6530 . + 0 ID=annotation1766;Name=luxR;Parent=BBa_C0062 BBa_I8003 . engineered_tag 6537 6561 . + 0 ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062 BBa_I8003 . point_mutation 6272 6272 . + 0 ID=annotation1765;Name=A;Parent=BBa_C0062 BBa_I8003 . engineered_region 5781 5782 . + 0 ID=annotation1762;Name=prefix;Parent=BBa_C0062 BBa_I8003 . engineered_region 5781 6536 . + 0 ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062 BBa_I8003 . point_mutation 5954 5954 . + 0 ID=annotation1764;Name=T;Parent=BBa_C0062 BBa_I8003 . ribosome_entry_site 1545 1556 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_I8003 . conserved_region 1549 1552 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_I8003 . ribosome_entry_site 5763 5774 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_I8003 . conserved_region 5767 5770 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_I8003 . promoter 1 55 . + 0 ID=BBa_R0062;Name=BBa_R0062 BBa_I8003 . non_covalent_binding_site 1 20 . + 0 ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062 BBa_I8003 . promoter 42 47 . + 0 ID=annotation2047;Name=-10;Parent=BBa_R0062 BBa_I8003 . promoter 20 25 . + 0 ID=annotation2046;Name=-35;Parent=BBa_R0062 BBa_I8003 . engineered_region 1 55 . + 0 ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062 BBa_I8003 . start_codon 53 53 . + 0 ID=annotation2048;Name=start;Parent=BBa_R0062 BBa_I8003 . ribosome_entry_site 3200 3211 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_I8003 . conserved_region 3204 3207 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 >BBa_I8003 acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatact agatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggcca caagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggc aagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccaca tgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacgg caactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttc aaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagc agaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactacca gcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaa gaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagc tgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagaaagaggagaaatactag atgatcgaactgctgtccgaatccctggaaggtctgtccgctgctatgatcgctgaactgggtcgttaccgtcaccagg ttttcatcgaaaaactgggttgggacgttgtttccacctcccgtgttcgtgaccaggagttcgaccagttcgaccaccc gcagacccgttacatcgttgctatgtcccgtcagggtatctgcggttgcgctcgtctgctgccgaccaccgacgcttac ctgctgaaagacgttttcgcttacctgtgctccgaaaccccgccgtccgacccgtccgtttgggaactgtcccgttacg ctgcttccgctgctgacgacccgcagctggctatgaaaatcttctggtcctccctccagtgcgcttggtacctgggtgc ttcctccgttgttgctgttaccaccaccgctatggaacgttacttcgttcgtaacggtgttatcctccagcgtctgggt ccgccgcagaaagttaaaggtgaaaccctggttgctatctccttcccggcttaccaggaacgtggtctggaaatgctgc tgcgttaccacccggaatggctccagggtgttccgctgtccatggctgttgctgcaaacgacgaaaactacgctttagt agcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatgactataatgataaaa aaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagac ttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttg tgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcct gaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagct caaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtat tacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattgga gacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcag tcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactaga gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcctgtgaaatctggcagtt accgttagctttcgaattggctaaaaagtgttctactagagaaagaggagaaatactagatggtgagcaagggcgagga gctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgag ggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggccca ccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaa gtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgag gtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctgg ggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaa cttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgac ggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatc acatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaa cgacgaaaactacgctttagtagcttaataatactagagaaagaggagaaatactagatgacagtaaagaagctttatt tcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaattattagacttacc ggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaagtgcagttaataat gaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattt taaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttgatcatgcaggaggaaa tggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaa gaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcata caccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattattaacgattgatgc atcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagctttatcttcaattaaa cgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaaaggggatgtaaag tgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatc gctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg tgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagt gatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgtccagat tagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaact cgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagcc attgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacg ctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaaca gtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagc gctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacaccta ctactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttatt cggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactac gctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatgaggaatg acggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgt cctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccg aagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccgg cgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaa cgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttcc gtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgc tgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagat cctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttc caccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctca tcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaaga ggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaat aatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctc attctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaattt aataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgct gtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcata cggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgc gtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaac aacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatat taggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaag tatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtaga tcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata