##gff-version 3
##sequence-region BBa_I763035 1 2155
BBa_I763035	.	engineered_region	1	1233	.	+	0	ID=BBa_I763025;Name=BBa_I763025
BBa_I763035	.	engineered_region	63	1233	.	+	0	ID=BBa_S0100;Name=BBa_S0100;Parent=BBa_I763025
BBa_I763035	.	CDS	81	1208	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_S0100
BBa_I763035	.	engineered_region	81	1208	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I763035	.	CDS	81	1208	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I763035	.	engineered_tag	1209	1233	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I763035	.	tag	1170	1208	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I763035	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S0100
BBa_I763035	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I763035	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I763025
BBa_I763035	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I763035	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I763035	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I763035	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I763035	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I763035	.	engineered_region	1242	2155	.	+	0	ID=BBa_I763020;Name=BBa_I763020
BBa_I763035	.	ribosome_entry_site	1242	1253	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I763020
BBa_I763035	.	conserved_region	1246	1249	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I763035	.	terminator	2115	2155	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I763020
BBa_I763035	.	polyA_site	2142	2155	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I763035	.	stem_loop	2122	2141	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I763035	.	engineered_region	2115	2155	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I763035	.	stop_codon	2148	2148	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I763035	.	engineered_region	1260	2018	.	+	0	ID=BBa_J04031;Name=BBa_J04031;Parent=BBa_I763020
BBa_I763035	.	tag	1974	2012	.	+	0	ID=annotation1507235;Name=LVA;Parent=BBa_J04031
BBa_I763035	.	start_codon	1260	1262	.	+	0	ID=annotation1507246;Name=Start;Parent=BBa_J04031
BBa_I763035	.	stop_codon	2013	2018	.	+	0	ID=annotation1507242;Name=Double Stop;Parent=BBa_J04031
BBa_I763035	.	engineered_region	1260	1979	.	+	0	ID=annotation1507202;Name=BBa_E0040;Parent=BBa_J04031
BBa_I763035	.	terminator	2027	2106	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I763020
BBa_I763035	.	stem_loop	2038	2081	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I763035	.	engineered_region	2027	2106	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_I763035
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtg
gtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattccca
accgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgcc
gtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagc
ggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctgg
atgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacc
catcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaa
atcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgca
atcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaa
tgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtcc
gggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaa
ccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggt
gaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccc
cgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaa
actacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatgcg
taaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttct
gtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctg
ttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagca
tgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaag
acacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatg
gaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatgg
aatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatact
ccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacg
aaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaag
gcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctca
gtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcg
ggtgggcctttctgcgtttata