##gff-version 3
##sequence-region BBa_I763028 1 4615
BBa_I763028	.	engineered_region	1	1370	.	+	0	ID=BBa_I763026;Name=BBa_I763026
BBa_I763028	.	terminator	1242	1370	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_I763026
BBa_I763028	.	terminator	1330	1370	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I763028	.	polyA_site	1357	1370	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I763028	.	engineered_region	1330	1370	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I763028	.	stem_loop	1337	1356	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I763028	.	stop_codon	1363	1363	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I763028	.	terminator	1242	1321	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I763028	.	stem_loop	1253	1296	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I763028	.	engineered_region	1242	1321	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I763028	.	engineered_region	1	1233	.	+	0	ID=BBa_I763025;Name=BBa_I763025;Parent=BBa_I763026
BBa_I763028	.	engineered_region	63	1233	.	+	0	ID=BBa_S0100;Name=BBa_S0100;Parent=BBa_I763025
BBa_I763028	.	CDS	81	1208	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_S0100
BBa_I763028	.	engineered_region	81	1208	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I763028	.	CDS	81	1208	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I763028	.	tag	1170	1208	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I763028	.	engineered_tag	1209	1233	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I763028	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S0100
BBa_I763028	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I763028	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I763025
BBa_I763028	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I763028	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I763028	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I763028	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I763028	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I763028	.	engineered_region	1379	4615	.	+	0	ID=BBa_I763019;Name=BBa_I763019
BBa_I763028	.	engineered_region	1379	3693	.	+	0	ID=BBa_I763016;Name=BBa_I763016;Parent=BBa_I763019
BBa_I763028	.	engineered_region	2388	3693	.	+	0	ID=BBa_I763015;Name=BBa_I763015;Parent=BBa_I763016
BBa_I763028	.	CDS	2406	3693	.	+	0	ID=BBa_J22101;Name=BBa_J22101;Parent=BBa_I763015
BBa_I763028	.	ribosome_entry_site	2388	2399	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I763015
BBa_I763028	.	conserved_region	2392	2395	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I763028	.	engineered_region	1379	2379	.	+	0	ID=BBa_I763005;Name=BBa_I763005;Parent=BBa_I763016
BBa_I763028	.	CDS	1605	2379	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_I763005
BBa_I763028	.	tag	2316	2348	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_I763028	.	CDS	1608	2315	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_I763028	.	engineered_tag	2355	2379	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_I763028	.	engineered_region	1379	1598	.	+	0	ID=BBa_J04500;Name=BBa_J04500;Parent=BBa_I763005
BBa_I763028	.	promoter	1379	1578	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04500
BBa_I763028	.	CDS	1379	1466	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_I763028	.	promoter	1515	1520	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_I763028	.	promoter	1539	1544	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_I763028	.	start_codon	1551	1551	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_I763028	.	non_covalent_binding_site	1467	1504	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_I763028	.	engineered_region	1379	1578	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_I763028	.	non_covalent_binding_site	1544	1578	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_I763028	.	ribosome_entry_site	1587	1598	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04500
BBa_I763028	.	conserved_region	1591	1594	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I763028	.	engineered_region	3702	4615	.	+	0	ID=BBa_I763020;Name=BBa_I763020;Parent=BBa_I763019
BBa_I763028	.	ribosome_entry_site	3702	3713	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I763020
BBa_I763028	.	conserved_region	3706	3709	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I763028	.	engineered_region	3720	4478	.	+	0	ID=BBa_J04031;Name=BBa_J04031;Parent=BBa_I763020
BBa_I763028	.	tag	4434	4472	.	+	0	ID=annotation1507235;Name=LVA;Parent=BBa_J04031
BBa_I763028	.	start_codon	3720	3722	.	+	0	ID=annotation1507246;Name=Start;Parent=BBa_J04031
BBa_I763028	.	engineered_region	3720	4439	.	+	0	ID=annotation1507202;Name=BBa_E0040;Parent=BBa_J04031
BBa_I763028	.	stop_codon	4473	4478	.	+	0	ID=annotation1507242;Name=Double Stop;Parent=BBa_J04031
BBa_I763028	.	terminator	4575	4615	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I763020
BBa_I763028	.	polyA_site	4602	4615	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I763028	.	engineered_region	4575	4615	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I763028	.	stem_loop	4582	4601	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I763028	.	stop_codon	4608	4608	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I763028	.	terminator	4487	4566	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I763020
BBa_I763028	.	stem_loop	4498	4541	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I763028	.	engineered_region	4487	4566	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_I763028
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtg
gtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattccca
accgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgcc
gtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagc
ggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctgg
atgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacc
catcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaa
atcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgca
atcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaa
tgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtcc
gggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaa
ccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggt
gaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccc
cgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaa
actacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcac
cttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaa
tgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcatt
aggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacata
ctagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgcc
ttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggca
gtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaa
gttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcac
ttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaa
aggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattcc
atgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagc
caggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtt
tttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagt
cagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagt
gtagatcgctactagagaaagaggagaaatactagatgtctgcccgtatttcgcgtaaggaaatccattatgtactatt
taaaaaacacaaacttttggatgttcggtttattctttttcttttacttttttatcatgggagcctacttcccgttttt
cccgatttggctacatgacatcaaccatatcagcaaaagtgatacgggtattatttttgccgctatttctctgttctcg
ctattattccaaccgctgtttggtctgctttctgacaaactcgggctgcgcaaatacctgctgtggattattaccggca
tgttagtgatgtttgcgccgttctttatttttatcttcgggccactgttacaatacaacattttagtaggatcgattgt
tggtggtatttatctaggcttttgttttaacgccggtgcgccagcagtagaggcatttattgagaaagtcagccgtcgc
agtaatttcgaatttggtcgcgcgcggatgtttggctgtgttggctgggcgctgtgtgcctcgattgtcggcatcatgt
tcaccatcaataatcagtttgttttctggctgggctctggctgtgcactcatcctcgccgttttactctttttcgccaa
aacggatgcgccctcttctgccacggttgccaatgcggtaggtgccaaccattcggcatttagccttaagctggcactg
gaactgttcagacagccaaaactgtggtttttgtcactgtatgttattggcgtttcctgcacctacgatgtttttgacc
aacagtttgctaatttctttacttcgttctttgctaccggtgaacagggtacgcgggtatttggctacgtaacgacaat
gggcgaattacttaacgcctcgattatgttctttgcgccactgatcattaatcgcatcggtgggaaaaacgccctgctg
ctggctggcactattatgtctgtacgtattattggctcatcgttcgccacctcagcgctggaagtggttattctgaaaa
cgctgcatatgtttgaagtaccgttcctgctggtgggctgctttaaatatattaccagccagtttgaagtgcgtttttc
agcgacgatttatctggtctgtttctgcttctttaagcaactggcgatgatttttatgtctgtactggcgggcaatatg
tatgaaagcatcggtttccagggcgcttatctggtgctgggtctggtggcgctgggcttcaccttaatttccgtgttca
cgcttagcggccccggcccgctttccctgctgcgtcgtcaggtgaatgaagtcgcttaatactagagaaagaggagaaa
tactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggc
acaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactgg
aaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcat
atgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacg
ggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattt
taaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaa
caaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatc
aacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaa
agatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaa
ctatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttata