##gff-version 3
##sequence-region BBa_I759017 1 1861
BBa_I759017	.	engineered_region	842	1861	.	+	0	ID=BBa_S03724;Name=BBa_S03724
BBa_I759017	.	engineered_region	979	1861	.	+	0	ID=BBa_S03708;Name=BBa_S03708;Parent=BBa_S03724
BBa_I759017	.	promoter	979	1013	.	+	0	ID=BBa_J23116;Name=BBa_J23116;Parent=BBa_S03708
BBa_I759017	.	engineered_region	1022	1861	.	+	0	ID=BBa_P0440;Name=BBa_P0440;Parent=BBa_S03708
BBa_I759017	.	terminator	1733	1812	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_I759017	.	stem_loop	1744	1787	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I759017	.	engineered_region	1733	1812	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I759017	.	ribosome_entry_site	1022	1033	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_I759017	.	conserved_region	1026	1029	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I759017	.	terminator	1821	1861	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_I759017	.	stem_loop	1828	1847	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I759017	.	stop_codon	1854	1854	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I759017	.	polyA_site	1848	1861	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I759017	.	engineered_region	1821	1861	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I759017	.	CDS	1040	1699	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_I759017	.	engineered_tag	1700	1724	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I759017	.	CDS	1043	1659	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I759017	.	tag	1660	1693	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I759017	.	terminator	842	970	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_S03724
BBa_I759017	.	terminator	842	921	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I759017	.	stem_loop	853	896	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I759017	.	engineered_region	842	921	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I759017	.	terminator	930	970	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I759017	.	stem_loop	937	956	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I759017	.	stop_codon	963	963	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I759017	.	polyA_site	957	970	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I759017	.	engineered_region	930	970	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I759017	.	engineered_region	1	833	.	+	0	ID=BBa_I759040;Name=BBa_I759040
BBa_I759017	.	sequence_feature	111	833	.	+	0	ID=annotation1958496;Name=YFP;Parent=BBa_I759040
BBa_I759017	.	sequence_feature	55	110	.	+	0	ID=annotation1958495;Name=cis5 + RBS;Parent=BBa_I759040
BBa_I759017	.	promoter	1	54	.	+	0	ID=annotation1958494;Name=pTet;Parent=BBa_I759040
>BBa_I759017
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacgtgcacaactgtttcacctcttgct
tttgggtaagacagaagaggagaaacaattgatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctg
gtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagc
tgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcct
gcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccag
gagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtga
accgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacag
ccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggac
ggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccact
acctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgc
cgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctca
gtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcg
ggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctagggactatgctagctactagagaaagag
gagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcg
aaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcg
ggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctgg
caagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttag
gtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactaga
gaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgct
aaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaag
gtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtc
cgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttata