##gff-version 3
##sequence-region BBa_I759009 1 1862
BBa_I759009	.	engineered_region	843	1862	.	+	0	ID=BBa_S03724;Name=BBa_S03724
BBa_I759009	.	engineered_region	980	1862	.	+	0	ID=BBa_S03708;Name=BBa_S03708;Parent=BBa_S03724
BBa_I759009	.	engineered_region	1023	1862	.	+	0	ID=BBa_P0440;Name=BBa_P0440;Parent=BBa_S03708
BBa_I759009	.	CDS	1041	1700	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_I759009	.	tag	1661	1694	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I759009	.	engineered_tag	1701	1725	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I759009	.	CDS	1044	1660	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I759009	.	terminator	1734	1813	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_I759009	.	stem_loop	1745	1788	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I759009	.	engineered_region	1734	1813	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I759009	.	ribosome_entry_site	1023	1034	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_I759009	.	conserved_region	1027	1030	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I759009	.	terminator	1822	1862	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_I759009	.	polyA_site	1849	1862	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I759009	.	engineered_region	1822	1862	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I759009	.	stem_loop	1829	1848	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I759009	.	stop_codon	1855	1855	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I759009	.	promoter	980	1014	.	+	0	ID=BBa_J23116;Name=BBa_J23116;Parent=BBa_S03708
BBa_I759009	.	terminator	843	971	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_S03724
BBa_I759009	.	terminator	931	971	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I759009	.	polyA_site	958	971	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I759009	.	engineered_region	931	971	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I759009	.	stem_loop	938	957	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I759009	.	stop_codon	964	964	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I759009	.	terminator	843	922	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I759009	.	stem_loop	854	897	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I759009	.	engineered_region	843	922	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I759009	.	engineered_region	1	834	.	+	0	ID=BBa_I759008;Name=BBa_I759008
BBa_I759009	.	sequence_feature	112	834	.	+	0	ID=annotation1955701;Name=YFP;Parent=BBa_I759008
BBa_I759009	.	sequence_feature	55	111	.	+	0	ID=annotation1958481;Name=spacer;Parent=BBa_I759008
BBa_I759009	.	promoter	1	54	.	+	0	ID=annotation1951920;Name=pTet;Parent=BBa_I759008
>BBa_I759009
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacgtgcacaattcactgagtcatatga
tcgatttgggtattaaagaggagaaacaattgatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcct
ggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaag
ctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcc
tgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtcca
ggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtg
aaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaaca
gccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgagga
cggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccac
tacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccg
ccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctc
agtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttc
gggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctagggactatgctagctactagagaaaga
ggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatc
gaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagc
gggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctg
gcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacattta
ggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactag
agaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgc
taaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaa
ggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggt
ccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccagg
catcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctact
agagtcacactggctcaccttcgggtgggcctttctgcgtttata