##gff-version 3
##sequence-region BBa_I751112 1 3334
BBa_I751112	.	tag	2430	2448	.	+	0	ID=BBa_J54025;Name=BBa_J54025
BBa_I751112	.	tag	2443	2448	.	+	0	ID=annotation1903322;Name=MulI;Parent=BBa_J54025
BBa_I751112	.	tag	2430	2434	.	+	0	ID=annotation1903321;Name=BglII;Parent=BBa_J54025
BBa_I751112	.	CDS	158	913	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_I751112	.	engineered_region	158	159	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I751112	.	engineered_region	158	913	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I751112	.	engineered_tag	914	938	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I751112	.	point_mutation	649	649	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I751112	.	point_mutation	331	331	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I751112	.	CDS	158	907	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I751112	.	ribosome_entry_site	2457	2471	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_I751112	.	ribosome_entry_site	2464	2468	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_I751112	.	engineered_region	2457	2471	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_I751112	.	ribosome_entry_site	2457	2471	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_I751112	.	tag	1084	1102	.	+	0	ID=BBa_J54033;Name=BBa_J54033
BBa_I751112	.	tag	1098	1102	.	+	0	ID=annotation1903324;Name=BamHI;Parent=BBa_J54033
BBa_I751112	.	tag	1084	1088	.	+	0	ID=annotation1903323;Name=SalI;Parent=BBa_J54033
BBa_I751112	.	promoter	1	151	.	+	0	ID=BBa_R0063;Name=BBa_R0063
BBa_I751112	.	engineered_region	1	151	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_I751112	.	start_codon	128	128	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_I751112	.	promoter	115	122	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_I751112	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_I751112	.	non_covalent_binding_site	130	149	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_I751112	.	promoter	89	94	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_I751112	.	terminator	1035	1075	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I751112	.	engineered_region	1035	1075	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I751112	.	stem_loop	1042	1061	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I751112	.	polyA_site	1062	1075	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I751112	.	stop_codon	1068	1068	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I751112	.	promoter	1111	1176	.	+	0	ID=BBa_I751501;Name=BBa_I751501
BBa_I751112	.	promoter	1129	1176	.	+	0	ID=annotation1955478;Name=plux-cI hybrid promoter;Parent=BBa_I751501
BBa_I751112	.	sequence_feature	1129	1135	.	+	0	ID=annotation1955479;Name=-35;Parent=BBa_I751501
BBa_I751112	.	non_covalent_binding_site	1111	1128	.	+	0	ID=annotation1955482;Name=luxR binding;Parent=BBa_I751501
BBa_I751112	.	non_covalent_binding_site	1158	1176	.	+	0	ID=annotation1955477;Name=OR1;Parent=BBa_I751501
BBa_I751112	.	non_covalent_binding_site	1136	1151	.	+	0	ID=annotation1955481;Name=OR2;Parent=BBa_I751501
BBa_I751112	.	sequence_feature	1152	1157	.	+	0	ID=annotation1955480;Name=-10;Parent=BBa_I751501
BBa_I751112	.	CDS	2478	3197	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_I751112	.	CDS	2478	3197	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_I751112	.	terminator	3206	3285	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I751112	.	engineered_region	3206	3285	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I751112	.	stem_loop	3217	3260	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I751112	.	sequence_feature	2398	2421	.	+	0	ID=BBa_I751410;Name=BBa_I751410
BBa_I751112	.	engineered_region	1219	2389	.	+	0	ID=BBa_S0100;Name=BBa_S0100
BBa_I751112	.	CDS	1237	2364	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_S0100
BBa_I751112	.	engineered_region	1237	2364	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I751112	.	engineered_tag	2365	2389	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I751112	.	tag	2326	2364	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I751112	.	CDS	1237	2364	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I751112	.	ribosome_entry_site	1219	1230	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S0100
BBa_I751112	.	conserved_region	1223	1226	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I751112	.	sequence_feature	1185	1210	.	+	0	ID=BBa_I751400;Name=BBa_I751400
BBa_I751112	.	terminator	3294	3334	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I751112	.	engineered_region	3294	3334	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I751112	.	stem_loop	3301	3320	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I751112	.	polyA_site	3321	3334	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I751112	.	stop_codon	3327	3327	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I751112	.	terminator	947	1026	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I751112	.	engineered_region	947	1026	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I751112	.	stem_loop	958	1001	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_I751112
acctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttat
tagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactaga
tgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatca
atgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaa
tctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatc
ctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatc
tccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggc
ttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacatac
cattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaa
aagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgag
cgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaa
ttttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagcc
aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct
actagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggtcgactgacgactggatctact
agagacctgtaggatcgtacaggtttacgtaacaccgtgcgtgttgatgcttttatcaccgccagtggtatactagagc
tagtgtcgactgacgactggatccatactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttat
acgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaa
aacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaa
cagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctc
gcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgca
caatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagct
gcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaag
acggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttc
tgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaa
ggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctgg
ttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggt
agtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctg
gggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcac
tggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagct
ggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctga
tagtgctagtgtagatctctactagagctagaagatctatcgtaacgcgtttactagaggatctgtcggataacgcgtt
actagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaa
ttagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttaccc
ttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatg
ctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaaga
actatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaa
tcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaa
tgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagc
gttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgt
ccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgg
gattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaa
agactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtggg
cctttctgcgtttata