##gff-version 3
##sequence-region BBa_I751111 1 2964
BBa_I751111	.	sequence_feature	2028	2051	.	+	0	ID=BBa_I751410;Name=BBa_I751410
BBa_I751111	.	ribosome_entry_site	2087	2101	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_I751111	.	engineered_region	2087	2101	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_I751111	.	ribosome_entry_site	2094	2098	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_I751111	.	ribosome_entry_site	2087	2101	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_I751111	.	terminator	2836	2915	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I751111	.	engineered_region	2836	2915	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I751111	.	stem_loop	2847	2890	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I751111	.	sequence_feature	1193	1218	.	+	0	ID=BBa_I751400;Name=BBa_I751400
BBa_I751111	.	promoter	1	151	.	+	0	ID=BBa_R0063;Name=BBa_R0063
BBa_I751111	.	promoter	89	94	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_I751111	.	non_covalent_binding_site	130	149	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_I751111	.	engineered_region	1	151	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_I751111	.	start_codon	128	128	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_I751111	.	promoter	115	122	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_I751111	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_I751111	.	CDS	2108	2827	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_I751111	.	CDS	2108	2827	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_I751111	.	tag	1084	1102	.	+	0	ID=BBa_J54033;Name=BBa_J54033
BBa_I751111	.	tag	1098	1102	.	+	0	ID=annotation1903324;Name=BamHI;Parent=BBa_J54033
BBa_I751111	.	tag	1084	1088	.	+	0	ID=annotation1903323;Name=SalI;Parent=BBa_J54033
BBa_I751111	.	tag	2060	2078	.	+	0	ID=BBa_J54025;Name=BBa_J54025
BBa_I751111	.	tag	2060	2064	.	+	0	ID=annotation1903321;Name=BglII;Parent=BBa_J54025
BBa_I751111	.	tag	2073	2078	.	+	0	ID=annotation1903322;Name=MulI;Parent=BBa_J54025
BBa_I751111	.	terminator	947	1026	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I751111	.	engineered_region	947	1026	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I751111	.	stem_loop	958	1001	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I751111	.	terminator	1035	1075	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I751111	.	stem_loop	1042	1061	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I751111	.	engineered_region	1035	1075	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I751111	.	stop_codon	1068	1068	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I751111	.	polyA_site	1062	1075	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I751111	.	promoter	1111	1184	.	+	0	ID=BBa_I751502;Name=BBa_I751502
BBa_I751111	.	non_covalent_binding_site	1111	1129	.	+	0	ID=annotation1955564;Name=luxR binding site;Parent=BBa_I751502
BBa_I751111	.	sequence_feature	1152	1158	.	+	0	ID=annotation1955561;Name=-10;Parent=BBa_I751502
BBa_I751111	.	sequence_feature	1130	1136	.	+	0	ID=annotation1955560;Name=-35;Parent=BBa_I751502
BBa_I751111	.	non_covalent_binding_site	1159	1184	.	+	0	ID=annotation1955563;Name=O1;Parent=BBa_I751502
BBa_I751111	.	promoter	1130	1184	.	+	0	ID=annotation1955559;Name=plac;Parent=BBa_I751502
BBa_I751111	.	non_covalent_binding_site	1137	1151	.	+	0	ID=annotation1955562;Name=O1;Parent=BBa_I751502
BBa_I751111	.	CDS	158	913	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_I751111	.	engineered_tag	914	938	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I751111	.	engineered_region	158	159	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I751111	.	point_mutation	331	331	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I751111	.	point_mutation	649	649	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I751111	.	engineered_region	158	913	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I751111	.	CDS	158	907	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I751111	.	terminator	2924	2964	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I751111	.	stem_loop	2931	2950	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I751111	.	engineered_region	2924	2964	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I751111	.	stop_codon	2957	2957	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I751111	.	polyA_site	2951	2964	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I751111	.	engineered_region	1227	2019	.	+	0	ID=BBa_S0103;Name=BBa_S0103
BBa_I751111	.	ribosome_entry_site	1227	1238	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S0103
BBa_I751111	.	conserved_region	1231	1234	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I751111	.	CDS	1245	1994	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_S0103
BBa_I751111	.	engineered_tag	1995	2019	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_I751111	.	CDS	1248	1955	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_I751111	.	tag	1956	1988	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
>BBa_I751111
acctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttat
tagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactaga
tgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatca
atgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaa
tctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatc
ctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatc
tccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggc
ttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacatac
cattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaa
aagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgag
cgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaa
ttttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagcc
aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct
actagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggtcgactgacgactggatctact
agagacctgtaggatcgtacaggtttacttgtgagcggataacaatatagtgtgtggaattgtgagcggataacaattt
actagagctagtgtcgactgacgactggatccatactagagaaagaggagaaatactagatgagcacaaaaaagaaacc
attaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcc
caggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatg
cttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatcta
cgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggca
gggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtg
attctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaat
gttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttacc
ttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatg
agagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaacta
cgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagctagaagatctatcgtaacgcgtttac
tagaggatctgtcggataacgcgttactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcac
tggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggt
gatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtca
ctactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccat
gcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagttt
gaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaat
tggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaat
tagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtc
cttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtcc
ttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatc
aaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagag
tcacactggctcaccttcgggtgggcctttctgcgtttata