##gff-version 3
##sequence-region BBa_I746315 1 2351
BBa_I746315	.	engineered_region	1	1482	.	+	0	ID=BBa_I746309;Name=BBa_I746309
BBa_I746315	.	engineered_region	1219	1482	.	+	0	ID=BBa_I746352;Name=BBa_I746352;Parent=BBa_I746309
BBa_I746315	.	start_codon	1240	1240	.	+	0	ID=annotation1943873;Name=phiR73 delta;Parent=BBa_I746352
BBa_I746315	.	sequence_alteration	1320	1320	.	+	0	ID=annotation1943874;Name=silent mutation to remove PstI site;Parent=BBa_I746352
BBa_I746315	.	engineered_region	1219	1230	.	+	0	ID=annotation1943871;Name=B0034;Parent=BBa_I746352
BBa_I746315	.	CDS	1240	1482	.	+	0	ID=annotation1943872;Name=phiR73 delta;Parent=BBa_I746352
BBa_I746315	.	promoter	1	1210	.	+	0	ID=BBa_I0500;Name=BBa_I0500;Parent=BBa_I746309
BBa_I746315	.	sequence_feature	1	1079	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_I746315	.	promoter	1043	1114	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_I746315	.	promoter	1080	1210	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_I746315	.	engineered_region	1491	2351	.	+	0	ID=BBa_I13507;Name=BBa_I13507
BBa_I746315	.	ribosome_entry_site	1491	1502	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_I746315	.	conserved_region	1495	1498	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I746315	.	terminator	2223	2302	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_I746315	.	engineered_region	2223	2302	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I746315	.	stem_loop	2234	2277	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I746315	.	CDS	1509	2189	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_I746315	.	CDS	1509	2183	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_I746315	.	engineered_tag	2190	2214	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_I746315	.	terminator	2311	2351	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_I746315	.	engineered_region	2311	2351	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I746315	.	stop_codon	2344	2344	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I746315	.	polyA_site	2338	2351	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I746315	.	stem_loop	2318	2337	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
>BBa_I746315
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat
tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg
tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa
aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga
tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc
gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg
cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag
ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac
cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac
caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa
ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg
cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg
cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa
gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac
tttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttc
tccatacccgtttttttgggctagctactagagaaagaggagaaatactagatgatgcgctgccctttctgtcgtcatt
cagcgcatacccgcaccagccggtatgtgagtgacaatgtcaaagaaagttatctccagtgccagaatatttactgttc
ggcgacatttaaaacgcatgagtcaatttgtgccgtgattcgttctccggtcacggaggaaaaaccagcaccggcaagc
acagcaccggctgttgtccgaaaagttaaaggctgttacagctcaccattcaaccattaatactagagaaagaggagaa
atactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggt
cacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaag
gtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggc
tgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggt
gttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcc
cgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgc
tctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatg
gctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactaca
ccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatc
gctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg
tgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata