##gff-version 3
##sequence-region BBa_I744214 1 2423
BBa_I744214	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I744214	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I744214	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I744214	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I744214	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I744214	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I744214	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I744214	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I744214	.	CDS	81	2423	.	+	0	ID=BBa_I744213;Name=BBa_I744213
BBa_I744214	.	sequence_feature	81	1406	.	+	0	ID=annotation1954581;Name=LuxN sensory domain fragment 2;Parent=BBa_I744213
BBa_I744214	.	start_codon	81	83	.	+	0	ID=annotation1954579;Name=Start translation;Parent=BBa_I744213
BBa_I744214	.	sequence_feature	1413	2423	.	+	0	ID=annotation1954582;Name=Tsr signaling domain fragment 2;Parent=BBa_I744213
BBa_I744214	.	stop_codon	2421	2423	.	+	0	ID=annotation1954580;Name=Stop translation;Parent=BBa_I744213
BBa_I744214	.	sequence_feature	1541	1541	.	+	0	ID=annotation1954583;Name=point mutation to remove natural NdeI;Parent=BBa_I744213
>BBa_I744214
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgtttgattttagcctagaggctatcgtctacgccaaagccattagcctcctcgcgaccgtggcagtcgtcatgatg
tggctcttctattactgctatcgacttaagcaaaaaaatgaagtgatctttggtactcaccacgcagcttatattgcgt
actcggtgtgtatcattgcgtggatcagcagcaatgcttatttccataccgatttactgcccgagctcggagcttcggc
tggcatgttcatggccaagtttgccaacctcgcttccttcttcgcatttgcttttgcctattatttctcatgccagttg
gcggcggagcaacgcaaaggcaaagtacaccgatggcagcaaggtatctttgtttcattaactgtgtactcactgttta
tcaatttgcgtcccggtttgaccgtagagcacgtcgatattgtcggcccaagtcaattcatcatcgagtttggccctca
tacctcttatttctttatcggcctcgttagctttgttgttctgactctcgtcaatcttgtcgccatgcgaacaaacagc
agtaagctgactctagccaagaccaactacatgatcgccggtatcttagtgtttatgctttcgacggcagtcattcatc
ttggtatgacttactttatgggagatttctcattgacttggttaccaccagccttgtcgataagtgaaatgctctttgt
cggctatgcactgctgacctcccgcttctacagcgtaaagtacattgcttacctcgctctcagcgtgctgctggtctgt
gctattttcgttctgcctttaggcgcaatattcatcccacttacggaaagtaatcagtggcttatcgccatacctatct
gcgcccttattggtattacttggcagcttttgtataaaaagaccagccgatatgcatcgttcttgatatatggcgacaa
gaaaacaccagtacagcaaatcttgtcacttgaagaggattttaagctttctattgatgatgcgatgcgccgtctgggt
aagctgctgcaaattccaaatgacaaactacgcctcgtaaccagtaactacaacgaaaccttttacgaagagtaccttt
catcaaaccgctcggtgttggtgtttgatgaactctctgaagagctcgaatacaaggtatcggcaaagcgttcaatgaa
ggcgctgtatgacaaaatgagctcaaacaacaccgctttggttatgccgctgtttgggcaaggtaaatcggttacgcac
ttattgatttctccccacaagagcaacaaccaaatgttctcgaacgaggaaatctcagcagttcatatggcctcgctgg
tagcgccaatgaatcgcctgattgacagcattcgtcatattgcaggcggcgatctggtgaaaccgattgaggtggatgg
ctctaatgagatggggcaactggcagagagtttgcgccacatgcagggagagctgatgcgtaccgtcggtgatgtgcgc
aacggggccaatgccatctatagcggtgccagcgaaatcgccaccggcaataacgatctctcttcgcgcaccgagcaac
aggccgcttcgctggaagagacggcagccagcatggagcaactgaccgcaacggtgaagcagaacgccgaaaatgcgcg
ccaggccagccatctggcgttaagtgcttctgaaacggcgcaacgcggcggtaaagtggtagataacgtggtgcagact
atgcgcgatatctccaccagttcgcagaaaatcgccgatattatcagcgtaattgacggcattgccttccagaccaata
ttctggctttgaacgcggcggttgaggctgcgcgtgcgggtgagcaagggcgcggttttgcggtggtcgcgggagaagt
gcgtaatctggcccagcgcagcgcccaggcggctcgtgaaattaaaagcctgattgaagactcggtggggaaagtggat
gttggctctacgctggtcgaaagcgccggggaaacaatggcggagattgtcagcgccgtgacccgcgtgacggacatta
tgggcgaaattgcttctgcttctgatgagcagagccgtggtatcgatcaggttggcttagcggttgctgagatggaccg
ggtaactcaacagaacgccgcgctggtggaagagtctgccgctgccgccgccgcgctggaagagcaggccagtcgcctg
accgaagcagtggcagtgttccggattcagcaacagcagcgtgaaacatcggctgtggtaaaaaccgtgacgccagctg
cgccgcgtaaaatggccgtggcagatagcgaggagaactgggaaacattttaa