##gff-version 3
##sequence-region BBa_I744121 1 958
BBa_I744121	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I744121	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I744121	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I744121	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I744121	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I744121	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I744121	.	engineered_region	63	958	.	+	0	ID=BBa_P0455;Name=BBa_P0455
BBa_I744121	.	CDS	81	830	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_P0455
BBa_I744121	.	engineered_tag	831	855	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_I744121	.	tag	792	824	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_I744121	.	CDS	84	791	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_I744121	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0455
BBa_I744121	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I744121	.	terminator	864	904	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0455
BBa_I744121	.	engineered_region	864	904	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I744121	.	stop_codon	897	897	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I744121	.	polyA_site	891	904	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I744121	.	stem_loop	871	890	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I744121	.	terminator	913	958	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_P0455
BBa_I744121	.	engineered_region	913	958	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_I744121	.	stem_loop	925	947	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
>BBa_I744121
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaag
aaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttattta
atggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagccc
ttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccct
gttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaa
gcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaa
gccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccaga
cttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagt
acccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttgg
cgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagtcacac
tggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttt
tttattattt