##gff-version 3
##sequence-region BBa_I742162 1 1486
BBa_I742162	.	CDS	627	1349	.	+	0	ID=BBa_E0030;Name=BBa_E0030
BBa_I742162	.	engineered_region	1	600	.	+	0	ID=BBa_J33207;Name=BBa_J33207
BBa_I742162	.	CDS	370	600	.	+	0	ID=annotation1907858;Name=lacZ';Parent=BBa_J33207
BBa_I742162	.	promoter	320	325	.	+	0	ID=annotation1907859;Name=-10;Parent=BBa_J33207
BBa_I742162	.	operator	332	352	.	+	0	ID=annotation1907856;Name=LacI binding site;Parent=BBa_J33207
BBa_I742162	.	operator	248	285	.	+	0	ID=annotation1907855;Name=CAP binding site;Parent=BBa_J33207
BBa_I742162	.	promoter	297	302	.	+	0	ID=annotation1907860;Name=-35;Parent=BBa_J33207
BBa_I742162	.	ribosome_entry_site	359	362	.	+	0	ID=annotation1907857;Name=rbs;Parent=BBa_J33207
BBa_I742162	.	sequence_feature	1	3	.	+	0	ID=annotation1907854;Name=SacI;Parent=BBa_J33207
BBa_I742162	.	terminator	1446	1486	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I742162	.	stop_codon	1479	1479	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I742162	.	stem_loop	1453	1472	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I742162	.	polyA_site	1473	1486	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I742162	.	engineered_region	1446	1486	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I742162	.	ribosome_entry_site	609	620	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I742162	.	conserved_region	613	616	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I742162	.	terminator	1358	1437	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I742162	.	stem_loop	1369	1412	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I742162	.	engineered_region	1358	1437	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_I742162
ctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctg
caactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgc
ccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaag
cgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggc
tcgtatgttgtgtgaaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacggattcactggc
cgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgcc
agctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttg
cctggtttccggcaccagaagcggtgccggaaagctggctggagtgatactagagaaagaggagaaatactagatggtg
agcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttca
gcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcc
cgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctg
cacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactaca
agacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggagga
cggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaac
ggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaaca
cccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaa
cgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaag
taataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttg
tcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata